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- W3128366974 abstract "• Specific gene knockdown in cerebellar Purkinje cells is a major challenge because Purkinje cells only make up a small fraction of the cerebellum and off-target effects of shRNA occur. • Little is known about the application of CRISPR-Cas13 and the resulting protein expression levels in Purkinje cells, a type of developing postmitotic neuron. • We explored the possibility of a Cas13-mediated conditional knockdown system for cerebellar Purkinje cells. This system can achieve a suppression of the target proteins restricted to Purkinje cells in mixed cerebellar cultures." @default.
- W3128366974 created "2021-02-15" @default.
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- W3128366974 date "2021-06-01" @default.
- W3128366974 modified "2023-10-06" @default.
- W3128366974 title "Conditional gene silencing via a CRISPR system in cerebellar Purkinje cells" @default.
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- W3128366974 doi "https://doi.org/10.1016/j.bbagen.2021.129869" @default.
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