Matches in SemOpenAlex for { <https://semopenalex.org/work/W3128587313> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W3128587313 abstract "Abstract BackgroundTNK2/ACK1, a non-receptor tyrosine kinase, plays critical roles in signalling transduces and trafficking. Our previous genome-wide CRISPR/CAS9 knockout screen revealed that mutant of TNK2 produced more restrict to influenza virus infection. In this study, we aim to illustrate the role of TNK2 for influenza A virus (IAV) replication in human cells.ResultsCRISPR/Cas9-mediated mutant of TNK2 resulted in a significant reduction in viral proteins expression and viral titres for multiple influenza strains, and furthermore, a decrease of nuclear import of IAV in the infected TNK2 mutant cells was observed in 3h post-infection. Interestingly, TNK2 mutation enhanced the colocalization of LC3 with autophagic receptor p62 and led to the attenuation of influenza virus-caused accumulation of autophagosomes in TNK2 mutant cells. Further, confocal microscopy visualization result showed that influenza viral matrix 2 (M2) was colocalized with Lamp1 in the infected TNK2 mutant cells in early infection, while almost no colocalization between M2 and Lamp1 was observed in IAV-infected wild-type cells. Moreover, TNK2 depletion also affected the trafficking of early endosome and the movement of influenza viral NP and M2.ConclusionsOur results identified TNK2 as a critical host factor for influenza viral M2 protein trafficking, suggesting that TNK2 will be an attractive target for the development of antivirals therapeutics." @default.
- W3128587313 created "2021-02-15" @default.
- W3128587313 creator A5025633711 @default.
- W3128587313 creator A5026611663 @default.
- W3128587313 creator A5051652070 @default.
- W3128587313 date "2021-01-29" @default.
- W3128587313 modified "2023-10-16" @default.
- W3128587313 title "TNK2/ACK1 Strengthen Influenza A Virus Infection by Blocking Viral Matrix 2 Protein(M2) into Lysosome to Degradation" @default.
- W3128587313 doi "https://doi.org/10.21203/rs.3.rs-153964/v1" @default.
- W3128587313 hasPublicationYear "2021" @default.
- W3128587313 type Work @default.
- W3128587313 sameAs 3128587313 @default.
- W3128587313 citedByCount "0" @default.
- W3128587313 crossrefType "posted-content" @default.
- W3128587313 hasAuthorship W3128587313A5025633711 @default.
- W3128587313 hasAuthorship W3128587313A5026611663 @default.
- W3128587313 hasAuthorship W3128587313A5051652070 @default.
- W3128587313 hasBestOaLocation W31285873131 @default.
- W3128587313 hasConcept C102747710 @default.
- W3128587313 hasConcept C104317684 @default.
- W3128587313 hasConcept C140704245 @default.
- W3128587313 hasConcept C143065580 @default.
- W3128587313 hasConcept C159047783 @default.
- W3128587313 hasConcept C2522874641 @default.
- W3128587313 hasConcept C2777546802 @default.
- W3128587313 hasConcept C40209533 @default.
- W3128587313 hasConcept C54355233 @default.
- W3128587313 hasConcept C58475186 @default.
- W3128587313 hasConcept C68434959 @default.
- W3128587313 hasConcept C79879829 @default.
- W3128587313 hasConcept C86803240 @default.
- W3128587313 hasConcept C95444343 @default.
- W3128587313 hasConceptScore W3128587313C102747710 @default.
- W3128587313 hasConceptScore W3128587313C104317684 @default.
- W3128587313 hasConceptScore W3128587313C140704245 @default.
- W3128587313 hasConceptScore W3128587313C143065580 @default.
- W3128587313 hasConceptScore W3128587313C159047783 @default.
- W3128587313 hasConceptScore W3128587313C2522874641 @default.
- W3128587313 hasConceptScore W3128587313C2777546802 @default.
- W3128587313 hasConceptScore W3128587313C40209533 @default.
- W3128587313 hasConceptScore W3128587313C54355233 @default.
- W3128587313 hasConceptScore W3128587313C58475186 @default.
- W3128587313 hasConceptScore W3128587313C68434959 @default.
- W3128587313 hasConceptScore W3128587313C79879829 @default.
- W3128587313 hasConceptScore W3128587313C86803240 @default.
- W3128587313 hasConceptScore W3128587313C95444343 @default.
- W3128587313 hasLocation W31285873131 @default.
- W3128587313 hasOpenAccess W3128587313 @default.
- W3128587313 hasPrimaryLocation W31285873131 @default.
- W3128587313 hasRelatedWork W1572227522 @default.
- W3128587313 hasRelatedWork W1974962646 @default.
- W3128587313 hasRelatedWork W2033005386 @default.
- W3128587313 hasRelatedWork W2103796054 @default.
- W3128587313 hasRelatedWork W2107663516 @default.
- W3128587313 hasRelatedWork W2110097466 @default.
- W3128587313 hasRelatedWork W2112798049 @default.
- W3128587313 hasRelatedWork W2497367789 @default.
- W3128587313 hasRelatedWork W3128587313 @default.
- W3128587313 hasRelatedWork W644858181 @default.
- W3128587313 isParatext "false" @default.
- W3128587313 isRetracted "false" @default.
- W3128587313 magId "3128587313" @default.
- W3128587313 workType "article" @default.