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- W3128689503 abstract "Dirofilariarepens is a parasitic nematode causing a vector-borne zoonotic infection (dirofilariosis), considered an emerging problem in human and veterinary medicine. Currently, diagnosis is based on the detection of the adult parasite and microfilariae in the host tissues. However, the efficacy of tests relying on microfilariae detection is limited by microfilariae periodic occurrence. Therefore, a new reliable and affordable serological diagnostic method is needed. Better characteristic of the parasite biology and its interaction with host immune system should help to achieve this goal. This study analyzes adult and microfilariae proteomes, and the use of one-dimensional electrophoresis (1-DE) and two-dimensional electrophoresis (2-DE) proteomics, immunoproteomics, and LC-MS/MS mass spectrometry allowed us to identify 316 potentially immunogenic proteins (75 belong to adult stage, 183 to microfilariae, and 58 are common for both). Classified by their ontology, the proteins showed important similarities and differences between both parasite stages. The most frequently identified proteins are structural, metabolic, and heat shock proteins. Additionally, real-time PCR analysis of some immunogenic targets revealed significant differences between microfilariae and adult life stages. We indicated molecules involved in parasite-host interactions and discussed their importance in parasite biology, which may help to reveal potential diagnostic antigens or select drug and vaccine targets." @default.
- W3128689503 created "2021-02-15" @default.
- W3128689503 creator A5007844491 @default.
- W3128689503 creator A5045487353 @default.
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- W3128689503 creator A5075296200 @default.
- W3128689503 creator A5076046189 @default.
- W3128689503 creator A5083533339 @default.
- W3128689503 date "2021-02-05" @default.
- W3128689503 modified "2023-10-14" @default.
- W3128689503 title "Immunoproteomic Analysis of Dirofilaria repens Microfilariae and Adult Parasite Stages" @default.
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