Matches in SemOpenAlex for { <https://semopenalex.org/work/W3128707549> ?p ?o ?g. }
- W3128707549 abstract "Abstract N-glycosylation is a ubiquitous posttranslational modification that may influence folding, subcellular localization, secretion, solubility and oligomerization of proteins. In this study, we examined the effects of N-glycans on the activity of human Gb3/CD77 synthase, which catalyzes the synthesis of glycosphingolipids with terminal Galα1→4Gal (Gb3 and the P1 antigen) and Galα1→4GalNAc disaccharides (the NOR antigen). The human Gb3/CD77 synthase contains two occupied N-glycosylation sites at positions N 121 and N 203 . Intriguingly, we found that while the N-glycan at N 203 is essential for activity and correct subcellular localization, the N-glycan at N 121 is dispensable and its absence did not reduce, but, surprisingly, even increased the activity of the enzyme. The fully N-glycosylated human Gb3/CD77 synthase and its glycoform missing the N 121 glycan correctly localized in the Golgi, whereas a glycoform without the N 203 site partially mislocalized in the endoplasmic reticulum. A double mutein missing both N-glycans was inactive and accumulated in the endoplasmic reticulum. Our results suggest that the decreased specific activity of human Gb3/CD77 synthase glycovariants results from their improper subcellular localization and, to a smaller degree, a decrease in enzyme solubility. Taken together, our findings show that the two N-glycans of human Gb3/CD77 synthase have opposing effects on its properties, revealing a dual nature of N-glycosylation and potentially a novel regulatory mechanism controlling the biological activity of proteins." @default.
- W3128707549 created "2021-02-15" @default.
- W3128707549 creator A5003449416 @default.
- W3128707549 creator A5011897079 @default.
- W3128707549 creator A5014092671 @default.
- W3128707549 creator A5031475050 @default.
- W3128707549 creator A5032338509 @default.
- W3128707549 creator A5047359007 @default.
- W3128707549 creator A5050753956 @default.
- W3128707549 creator A5051297197 @default.
- W3128707549 creator A5062583016 @default.
- W3128707549 creator A5062690358 @default.
- W3128707549 creator A5073067478 @default.
- W3128707549 creator A5075885587 @default.
- W3128707549 creator A5086553812 @default.
- W3128707549 creator A5088847951 @default.
- W3128707549 date "2021-02-04" @default.
- W3128707549 modified "2023-10-02" @default.
- W3128707549 title "Missing the sweet spot: one of the two N-glycans on human Gb3/CD77 synthase is expendable" @default.
- W3128707549 cites W1146978950 @default.
- W3128707549 cites W120298262 @default.
- W3128707549 cites W135183689 @default.
- W3128707549 cites W145329232 @default.
- W3128707549 cites W1491752347 @default.
- W3128707549 cites W1520145304 @default.
- W3128707549 cites W154246096 @default.
- W3128707549 cites W1865473340 @default.
- W3128707549 cites W1976452565 @default.
- W3128707549 cites W1978768249 @default.
- W3128707549 cites W1986169495 @default.
- W3128707549 cites W1995907677 @default.
- W3128707549 cites W2000987281 @default.
- W3128707549 cites W2012659268 @default.
- W3128707549 cites W2012864276 @default.
- W3128707549 cites W2013075345 @default.
- W3128707549 cites W2021308715 @default.
- W3128707549 cites W2026084043 @default.
- W3128707549 cites W2029489645 @default.
- W3128707549 cites W2032659602 @default.
- W3128707549 cites W2033331583 @default.
- W3128707549 cites W2039506690 @default.
- W3128707549 cites W2048052435 @default.
- W3128707549 cites W2061255585 @default.
- W3128707549 cites W2075620734 @default.
- W3128707549 cites W2081448012 @default.
- W3128707549 cites W2091847404 @default.
- W3128707549 cites W2100837269 @default.
- W3128707549 cites W2101108802 @default.
- W3128707549 cites W2105081087 @default.
- W3128707549 cites W2110234748 @default.
- W3128707549 cites W2114742973 @default.
- W3128707549 cites W2122559203 @default.
- W3128707549 cites W2127227088 @default.
- W3128707549 cites W2137469126 @default.
- W3128707549 cites W2139958335 @default.
- W3128707549 cites W2140022324 @default.
- W3128707549 cites W2147769824 @default.
- W3128707549 cites W2222175439 @default.
- W3128707549 cites W2296880033 @default.
- W3128707549 cites W2325999336 @default.
- W3128707549 cites W2413008383 @default.
- W3128707549 cites W2510483670 @default.
- W3128707549 cites W2515063788 @default.
- W3128707549 cites W2554188434 @default.
- W3128707549 cites W2564893946 @default.
- W3128707549 cites W2609844681 @default.
- W3128707549 cites W2731464816 @default.
- W3128707549 cites W2767187208 @default.
- W3128707549 cites W2794280235 @default.
- W3128707549 cites W2802279695 @default.
- W3128707549 cites W2897845851 @default.
- W3128707549 cites W2905813089 @default.
- W3128707549 cites W2907858220 @default.
- W3128707549 cites W2908431314 @default.
- W3128707549 cites W2936815238 @default.
- W3128707549 cites W2952073529 @default.
- W3128707549 cites W2972821158 @default.
- W3128707549 cites W3014163652 @default.
- W3128707549 cites W3023270555 @default.
- W3128707549 cites W3024927906 @default.
- W3128707549 cites W3035124406 @default.
- W3128707549 cites W3081165619 @default.
- W3128707549 cites W3108405639 @default.
- W3128707549 cites W3116593683 @default.
- W3128707549 cites W3119565936 @default.
- W3128707549 cites W4234833860 @default.
- W3128707549 cites W2138903313 @default.
- W3128707549 doi "https://doi.org/10.1101/2021.02.04.429793" @default.
- W3128707549 hasPublicationYear "2021" @default.
- W3128707549 type Work @default.
- W3128707549 sameAs 3128707549 @default.
- W3128707549 citedByCount "0" @default.
- W3128707549 crossrefType "posted-content" @default.
- W3128707549 hasAuthorship W3128707549A5003449416 @default.
- W3128707549 hasAuthorship W3128707549A5011897079 @default.
- W3128707549 hasAuthorship W3128707549A5014092671 @default.
- W3128707549 hasAuthorship W3128707549A5031475050 @default.
- W3128707549 hasAuthorship W3128707549A5032338509 @default.
- W3128707549 hasAuthorship W3128707549A5047359007 @default.
- W3128707549 hasAuthorship W3128707549A5050753956 @default.