FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3128865957> ?p ?o ?g. }

• W3128865957 abstract "Abstract The feline calicivirus (FCV), feline herspesvirus 1 (FHV-1) and feline panleukemia virus (FPV) are heavily threaten the health of cats. In this study, a triplex TaqMan real-time polymerase chain reaction (RT-PCR) assay (triplex assay) was developed to detect these viruses. The optimized concentration of primers was 0.5 µM of each, probes concentration was 0.1 µM for FCV and FHV-1, 0.05 µM for FPV. The annealing temperature was set at 54 ℃. The triplex RT-PCR assay was carefully validated. The detection limit for FPV, FCV, and FHV-1 was 5×10 1 copies/µL, which showed a 10-100-fold increase in the sensitivity compared with the conventional PCR. The coefficients of variation (CV) of the intra-assay variability of the test were < 1.86%, and that of inter-assay was < 3.19%, indicating excellent repeatability and reproducibility of the triplex assay. Additionally, the assay has perfect specificity. In a pilot study, samples from 48 cats were analyzed using the triplex RT-PCR method and the commercial kits, and further confirmed by sequencing. The positive rates for the samples analyzed with these two methods were 70.83% and 62.5%, respectively, which demonstrated that the developed method was more accurate than the commercial kits in clinical diagnosis." @default.
• W3128865957 created "2021-02-15" @default.
• W3128865957 creator A5012515727 @default.
• W3128865957 creator A5021850946 @default.
• W3128865957 creator A5026737536 @default.
• W3128865957 creator A5030274499 @default.
• W3128865957 creator A5082947695 @default.
• W3128865957 date "2021-02-10" @default.
• W3128865957 modified "2023-09-27" @default.
• W3128865957 title "Development and application of a triplex TaqMan RT-PCR assay for simultaneous detection of Feline calicivirus, Feline Herpesvirus 1 and Feline parvovirus" @default.
• W3128865957 cites W1120172167 @default.
• W3128865957 cites W134747768 @default.
• W3128865957 cites W148895522 @default.
• W3128865957 cites W1963626983 @default.
• W3128865957 cites W1970660259 @default.
• W3128865957 cites W1975733629 @default.
• W3128865957 cites W2007186418 @default.
• W3128865957 cites W2014221824 @default.
• W3128865957 cites W2025453603 @default.
• W3128865957 cites W2064738367 @default.
• W3128865957 cites W2068874460 @default.
• W3128865957 cites W2072535932 @default.
• W3128865957 cites W2076258503 @default.
• W3128865957 cites W2094596871 @default.
• W3128865957 cites W2100187217 @default.
• W3128865957 cites W2124730317 @default.
• W3128865957 cites W2144677167 @default.
• W3128865957 cites W2158894328 @default.
• W3128865957 cites W2165136402 @default.
• W3128865957 cites W2168420558 @default.
• W3128865957 cites W2467721711 @default.
• W3128865957 cites W2942835677 @default.
• W3128865957 doi "https://doi.org/10.21203/rs.3.rs-205583/v1" @default.
• W3128865957 hasPublicationYear "2021" @default.
• W3128865957 type Work @default.
• W3128865957 sameAs 3128865957 @default.
• W3128865957 citedByCount "0" @default.
• W3128865957 crossrefType "posted-content" @default.
• W3128865957 hasAuthorship W3128865957A5012515727 @default.
• W3128865957 hasAuthorship W3128865957A5021850946 @default.
• W3128865957 hasAuthorship W3128865957A5026737536 @default.
• W3128865957 hasAuthorship W3128865957A5030274499 @default.
• W3128865957 hasAuthorship W3128865957A5082947695 @default.
• W3128865957 hasBestOaLocation W31288659571 @default.
• W3128865957 hasConcept C104317684 @default.
• W3128865957 hasConcept C119128265 @default.
• W3128865957 hasConcept C126322002 @default.
• W3128865957 hasConcept C143660080 @default.
• W3128865957 hasConcept C153911025 @default.
• W3128865957 hasConcept C154020017 @default.
• W3128865957 hasConcept C159047783 @default.
• W3128865957 hasConcept C185592680 @default.
• W3128865957 hasConcept C2522874641 @default.
• W3128865957 hasConcept C2777747160 @default.
• W3128865957 hasConcept C2779630601 @default.
• W3128865957 hasConcept C2779904517 @default.
• W3128865957 hasConcept C2780339557 @default.
• W3128865957 hasConcept C2780727368 @default.
• W3128865957 hasConcept C2780980013 @default.
• W3128865957 hasConcept C2909870366 @default.
• W3128865957 hasConcept C43617362 @default.
• W3128865957 hasConcept C48023723 @default.
• W3128865957 hasConcept C49105822 @default.
• W3128865957 hasConcept C54355233 @default.
• W3128865957 hasConcept C71924100 @default.
• W3128865957 hasConcept C86803240 @default.
• W3128865957 hasConceptScore W3128865957C104317684 @default.
• W3128865957 hasConceptScore W3128865957C119128265 @default.
• W3128865957 hasConceptScore W3128865957C126322002 @default.
• W3128865957 hasConceptScore W3128865957C143660080 @default.
• W3128865957 hasConceptScore W3128865957C153911025 @default.
• W3128865957 hasConceptScore W3128865957C154020017 @default.
• W3128865957 hasConceptScore W3128865957C159047783 @default.
• W3128865957 hasConceptScore W3128865957C185592680 @default.
• W3128865957 hasConceptScore W3128865957C2522874641 @default.
• W3128865957 hasConceptScore W3128865957C2777747160 @default.
• W3128865957 hasConceptScore W3128865957C2779630601 @default.
• W3128865957 hasConceptScore W3128865957C2779904517 @default.
• W3128865957 hasConceptScore W3128865957C2780339557 @default.
• W3128865957 hasConceptScore W3128865957C2780727368 @default.
• W3128865957 hasConceptScore W3128865957C2780980013 @default.
• W3128865957 hasConceptScore W3128865957C2909870366 @default.
• W3128865957 hasConceptScore W3128865957C43617362 @default.
• W3128865957 hasConceptScore W3128865957C48023723 @default.
• W3128865957 hasConceptScore W3128865957C49105822 @default.
• W3128865957 hasConceptScore W3128865957C54355233 @default.
• W3128865957 hasConceptScore W3128865957C71924100 @default.
• W3128865957 hasConceptScore W3128865957C86803240 @default.
• W3128865957 hasLocation W31288659571 @default.
• W3128865957 hasOpenAccess W3128865957 @default.
• W3128865957 hasPrimaryLocation W31288659571 @default.
• W3128865957 hasRelatedWork W1994000467 @default.
• W3128865957 hasRelatedWork W1998055757 @default.
• W3128865957 hasRelatedWork W2044815204 @default.
• W3128865957 hasRelatedWork W2049943154 @default.
• W3128865957 hasRelatedWork W2943197757 @default.
• W3128865957 hasRelatedWork W3083531937 @default.
• W3128865957 hasRelatedWork W3128865957 @default.
• W3128865957 hasRelatedWork W3193606861 @default.
• W3128865957 hasRelatedWork W4210859526 @default.

• next