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- W3128931079 abstract "Spread of drug-resistant bacteria is a serious problem worldwide. We thus designed a new sequence-based protocol that can quickly identify bacterial compositions of clinical samples and their drug-resistance profiles simultaneously. Here we utilized propidium monoazide (PMA) that prohibits DNA amplifications from dead bacteria, and subjected the original and antibiotics-treated samples to 16S rRNA metagenome sequencing. We tested our protocol on bacterial mixtures, and observed that sequencing reads derived from drug-resistant bacteria were significantly increased compared with those from drug-sensitive bacteria when samples were treated by antibiotics. Our protocol is scalable and will be useful for quickly profiling drug-resistant bacteria." @default.
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- W3128931079 date "2021-02-09" @default.
- W3128931079 modified "2023-09-25" @default.
- W3128931079 title "Rapid profiling of drug-resistant bacteria using DNA-binding dyes and a nanopore-based DNA sequencer" @default.
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- W3128931079 doi "https://doi.org/10.1038/s41598-021-82903-z" @default.
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