Matches in SemOpenAlex for { <https://semopenalex.org/work/W3128931385> ?p ?o ?g. }
- W3128931385 endingPage "1837" @default.
- W3128931385 startingPage "1825" @default.
- W3128931385 abstract "Cholesterol is a ubiquitous component of mammalian cell membranes and affects membrane protein function. Although cholesterol-mediated formation of ordered membrane domains has been extensively studied, molecular-level structural information about cholesterol self-association has been absent. Here, we combine solid-state nuclear magnetic resonance (NMR) spectroscopy with all-atom molecular dynamics simulations to determine the oligomeric structure of cholesterol in phospholipid bilayers. Two-dimensional 13C-13C correlation spectra of differentially labeled cholesterol indicate that cholesterol self-associates in a face-to-face fashion at membrane concentrations from 17 to 44 mol %. 2D 13C and 19F spin-counting experiments allowed us to measure the average oligomeric number of these cholesterol clusters. At low cholesterol concentrations of ∼20%, the average cluster size is centered on dimers. At a high cholesterol concentration of 44%, which is representative of virus lipid envelopes and liquid-ordered domains of cell membranes, both dimers and tetramers are observed. The cholesterol dimers are found in both phase-separated membranes that contain sphingomyelin and in disordered and miscible membranes that are free of sphingomyelin. Molecular dynamics simulations support these experimental observations and moreover provide the lifetimes, stabilities, distributions, and structures of these nanoscopic cholesterol clusters. Taken together, these NMR and MD data strongly suggest that dimers are the basic structural unit of cholesterol in phospholipid bilayers. The direct observation of cholesterol dimers and tetramers provides a revised framework for studying cholesterol interactions with membrane proteins to regulate protein functions and for understanding the pathogenic role of cholesterol in diseases." @default.
- W3128931385 created "2021-02-15" @default.
- W3128931385 creator A5013024407 @default.
- W3128931385 creator A5013534890 @default.
- W3128931385 creator A5029522460 @default.
- W3128931385 creator A5058069370 @default.
- W3128931385 creator A5077070129 @default.
- W3128931385 date "2021-02-09" @default.
- W3128931385 modified "2023-10-16" @default.
- W3128931385 title "Direct Observation of Cholesterol Dimers and Tetramers in Lipid Bilayers" @default.
- W3128931385 cites W1031578623 @default.
- W3128931385 cites W1765065472 @default.
- W3128931385 cites W1918796765 @default.
- W3128931385 cites W1965984853 @default.
- W3128931385 cites W1970541648 @default.
- W3128931385 cites W1976499671 @default.
- W3128931385 cites W1979166300 @default.
- W3128931385 cites W1982909744 @default.
- W3128931385 cites W1986892064 @default.
- W3128931385 cites W1990771438 @default.
- W3128931385 cites W1991794210 @default.
- W3128931385 cites W1996989293 @default.
- W3128931385 cites W1997772366 @default.
- W3128931385 cites W1999906821 @default.
- W3128931385 cites W2000523053 @default.
- W3128931385 cites W2001052983 @default.
- W3128931385 cites W2001524271 @default.
- W3128931385 cites W2005108951 @default.
- W3128931385 cites W2013899608 @default.
- W3128931385 cites W2015293263 @default.
- W3128931385 cites W2017196167 @default.
- W3128931385 cites W2021520922 @default.
- W3128931385 cites W2021882008 @default.
- W3128931385 cites W2024372419 @default.
- W3128931385 cites W2025027447 @default.
- W3128931385 cites W2028803516 @default.
- W3128931385 cites W2029667189 @default.
- W3128931385 cites W2035687084 @default.
- W3128931385 cites W2037115572 @default.
- W3128931385 cites W2038769526 @default.
- W3128931385 cites W2039305966 @default.
- W3128931385 cites W2049185462 @default.
- W3128931385 cites W2050199670 @default.
- W3128931385 cites W2052744592 @default.
- W3128931385 cites W2059013803 @default.
- W3128931385 cites W2059382533 @default.
- W3128931385 cites W2060433756 @default.
- W3128931385 cites W2065158535 @default.
- W3128931385 cites W2066414494 @default.
- W3128931385 cites W2067246066 @default.
- W3128931385 cites W2067520392 @default.
- W3128931385 cites W2070103021 @default.
- W3128931385 cites W2070753604 @default.
- W3128931385 cites W2072917759 @default.
- W3128931385 cites W2074218685 @default.
- W3128931385 cites W2075629818 @default.
- W3128931385 cites W2081693079 @default.
- W3128931385 cites W2083725367 @default.
- W3128931385 cites W2088603936 @default.
- W3128931385 cites W2092508662 @default.
- W3128931385 cites W2098792909 @default.
- W3128931385 cites W2115183962 @default.
- W3128931385 cites W2125872855 @default.
- W3128931385 cites W2134866108 @default.
- W3128931385 cites W2135177750 @default.
- W3128931385 cites W2136558879 @default.
- W3128931385 cites W2144676492 @default.
- W3128931385 cites W2153856953 @default.
- W3128931385 cites W2157359366 @default.
- W3128931385 cites W2157957881 @default.
- W3128931385 cites W2164763140 @default.
- W3128931385 cites W2170711116 @default.
- W3128931385 cites W2171832928 @default.
- W3128931385 cites W2171909837 @default.
- W3128931385 cites W2173374829 @default.
- W3128931385 cites W2284234508 @default.
- W3128931385 cites W2313545945 @default.
- W3128931385 cites W2313973094 @default.
- W3128931385 cites W2330260612 @default.
- W3128931385 cites W2402894416 @default.
- W3128931385 cites W2444060841 @default.
- W3128931385 cites W2514856898 @default.
- W3128931385 cites W2535769768 @default.
- W3128931385 cites W2546702170 @default.
- W3128931385 cites W2564298127 @default.
- W3128931385 cites W2601080948 @default.
- W3128931385 cites W2751124369 @default.
- W3128931385 cites W2770725028 @default.
- W3128931385 cites W2794233537 @default.
- W3128931385 cites W2802301891 @default.
- W3128931385 cites W2805301140 @default.
- W3128931385 cites W2896686597 @default.
- W3128931385 cites W2910356247 @default.
- W3128931385 cites W2911785287 @default.
- W3128931385 cites W2945447986 @default.
- W3128931385 cites W2945699073 @default.
- W3128931385 cites W2951489519 @default.
- W3128931385 cites W3082930782 @default.