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• W3128988617 abstract "Genomic selection (GS) currently represents the hot research area in aquaculture, but implementing GS in some aquatic animals is still hampered by the lacking of efficient nonlethal DNA sampling approach for genome-wide genotyping. This is particularly challenging for bivalve molluscs as they generally have small body size and are vulnerable to mass-tissue sampling methods. Here we describe an integrated approach for rapid, cost-effective nonlethal gill DNA sampling and genome-scale genotyping in scallop. The nonlethal DNA sampling procedure can be finished within 20 min and bypass many tedious and time-consuming steps in the traditional phenol-chloroform method and without using any commercial kit. Experimental optimization of nonlethal gill DNA sampling for genome-wide genotyping was achieved by taking advantage of orthogonal array testing for conquering the complexity of factor combinations. Based on the orthogonal array testing and preferred reduction of gill usage, the best configuration of tissue lysis for direct DNA use without any purification steps was determined, and its superior genotyping performance and high reproducibility were validated by using the high-throughput, cost-efficient 2b-RAD sequencing. We also demonstrated the great applicability of our method on other bivalves. Taken together, our study provides a highly efficient integrated approach of nonlethal DNA sampling and genome-wide genotyping for implementation of GS in shellfish aquaculture." @default.
• W3128988617 created "2021-02-15" @default.
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• W3128988617 date "2021-04-01" @default.
• W3128988617 modified "2023-10-16" @default.
• W3128988617 title "An efficient integrated approach for nonlethal DNA sampling and genome-wide genotyping in bivalve molluscs" @default.
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• W3128988617 doi "https://doi.org/10.1016/j.aquaculture.2021.736489" @default.
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