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- W3128992351 abstract "Abstract Complement is an important effector mechanism for antibody-mediated clearance of infections and tumor cells. Upon binding to target cells, the antibody’s constant (Fc) domain recruits complement component C1 to initiate a proteolytic cascade that generates lytic pores and stimulates phagocytosis. The C1 complex (C1qr 2 s 2 ) consists of the large recognition protein C1q and a heterotetramer of proteases C1r and C1s (C1r 2 s 2 ). While interactions between C1 and IgG-Fc’s are believed to be mediated by the globular heads of C1q, we here find that C1r 2 s 2 proteases affect the capacity of C1q to form an avid complex with surface-bound IgG molecules (on various DNP-coated surfaces and pathogenic Staphylococcus aureus ). The extent to which C1r 2 s 2 contribute to C1q-IgG stability strongly differs between human IgG subclasses. Using antibody engineering of monoclonal IgG we reveal that hexamer-enhancing mutations improve C1q-IgG stability, both in absence and presence of C1r 2 s 2 . In addition, hexamer-enhanced IgGs targeting S. aureus mediate improved complement-dependent phagocytosis by human neutrophils. Altogether, these molecular insights into complement binding to surface-bound IgGs could be important for optimal design of antibody therapies." @default.
- W3128992351 created "2021-02-15" @default.
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- W3128992351 date "2021-02-10" @default.
- W3128992351 modified "2023-09-26" @default.
- W3128992351 title "C1q binding to surface-bound IgG is stabilized by C1r2s2 proteases" @default.
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