Matches in SemOpenAlex for { <https://semopenalex.org/work/W3129121462> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W3129121462 abstract "The increasing resistance of staphylococci to ß -lactam antibiotics has become a major clinical problem. Development of rapid and sensitive techniques for detection of MRSA is an important aim for public health. A duplex PCR were established for specific identification of methicillin-resistance Staphylococcus aureus (MRSA) in clinical samples. In this work a duplex SYBR Green real time PCR was developed for rapid identification of MRSA in local methicillin-resistance S. aureus isolates. Twenty methicillin-resistance S. aureus isolates, as determined by disc diffusion method, were subjected to DNA extraction and PCR amplification. Two genes were amplified successfully, mecA (533bp) and femA (314bp), as targets for methicillin-resistance and specific identification of S. aureus, respectively using conventional PCR. Sensitivity of the duplex PCR showed that the minimum concentration of DNA that gave positive results for the two genes was 30ng/µl. In order to develop rapid and sensitive test for identification of MRSA, serial dilutions of purified DNA were amplified gradually according to their concentrations using SYBR Green real time PCR. These results indicated that the SYBR Green real time PCR can be used for identification of methicillin-resistance S. aureus (MRSA) in clinical" @default.
- W3129121462 created "2021-02-15" @default.
- W3129121462 creator A5020058724 @default.
- W3129121462 creator A5022990456 @default.
- W3129121462 creator A5052395982 @default.
- W3129121462 creator A5061934525 @default.
- W3129121462 date "2013-01-01" @default.
- W3129121462 modified "2023-09-27" @default.
- W3129121462 title "Development of SYBR Green Real time PCR for identification methicillin-resistance Staphylococcus aureus (MRSA)" @default.
- W3129121462 cites W2051438193 @default.
- W3129121462 cites W2069668456 @default.
- W3129121462 cites W2091404988 @default.
- W3129121462 cites W2102419473 @default.
- W3129121462 cites W2103647500 @default.
- W3129121462 doi "https://doi.org/10.24126/jobrc.2013.7.1.248" @default.
- W3129121462 hasPublicationYear "2013" @default.
- W3129121462 type Work @default.
- W3129121462 sameAs 3129121462 @default.
- W3129121462 citedByCount "2" @default.
- W3129121462 countsByYear W31291214622015 @default.
- W3129121462 countsByYear W31291214622016 @default.
- W3129121462 crossrefType "journal-article" @default.
- W3129121462 hasAuthorship W3129121462A5020058724 @default.
- W3129121462 hasAuthorship W3129121462A5022990456 @default.
- W3129121462 hasAuthorship W3129121462A5052395982 @default.
- W3129121462 hasAuthorship W3129121462A5061934525 @default.
- W3129121462 hasBestOaLocation W31291214621 @default.
- W3129121462 hasConcept C116834253 @default.
- W3129121462 hasConcept C2777052132 @default.
- W3129121462 hasConcept C2779489039 @default.
- W3129121462 hasConcept C523546767 @default.
- W3129121462 hasConcept C54355233 @default.
- W3129121462 hasConcept C59822182 @default.
- W3129121462 hasConcept C86803240 @default.
- W3129121462 hasConcept C89423630 @default.
- W3129121462 hasConceptScore W3129121462C116834253 @default.
- W3129121462 hasConceptScore W3129121462C2777052132 @default.
- W3129121462 hasConceptScore W3129121462C2779489039 @default.
- W3129121462 hasConceptScore W3129121462C523546767 @default.
- W3129121462 hasConceptScore W3129121462C54355233 @default.
- W3129121462 hasConceptScore W3129121462C59822182 @default.
- W3129121462 hasConceptScore W3129121462C86803240 @default.
- W3129121462 hasConceptScore W3129121462C89423630 @default.
- W3129121462 hasLocation W31291214621 @default.
- W3129121462 hasOpenAccess W3129121462 @default.
- W3129121462 hasPrimaryLocation W31291214621 @default.
- W3129121462 hasRelatedWork W1488610417 @default.
- W3129121462 hasRelatedWork W1981230192 @default.
- W3129121462 hasRelatedWork W1993325664 @default.
- W3129121462 hasRelatedWork W2022042030 @default.
- W3129121462 hasRelatedWork W2061030755 @default.
- W3129121462 hasRelatedWork W2117063197 @default.
- W3129121462 hasRelatedWork W2118847820 @default.
- W3129121462 hasRelatedWork W2130785355 @default.
- W3129121462 hasRelatedWork W2146673868 @default.
- W3129121462 hasRelatedWork W2310134273 @default.
- W3129121462 hasRelatedWork W2360093599 @default.
- W3129121462 hasRelatedWork W2371062592 @default.
- W3129121462 hasRelatedWork W2376043291 @default.
- W3129121462 hasRelatedWork W2376224906 @default.
- W3129121462 hasRelatedWork W2388184628 @default.
- W3129121462 hasRelatedWork W2388271309 @default.
- W3129121462 hasRelatedWork W2624729620 @default.
- W3129121462 hasRelatedWork W2786600727 @default.
- W3129121462 hasRelatedWork W2953180633 @default.
- W3129121462 hasRelatedWork W2837807433 @default.
- W3129121462 isParatext "false" @default.
- W3129121462 isRetracted "false" @default.
- W3129121462 magId "3129121462" @default.
- W3129121462 workType "article" @default.