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• W3129129219 abstract "Base-pairing interactions mediate many intermolecular target recognition events. Even a single base-pair mismatch can cause a substantial difference in activity but how such changes influence the target search kinetics in vivo is unknown. Here, we use high-throughput sequencing and quantitative super-resolution imaging to probe the mutants of bacterial small RNA, SgrS, and their regulation of ptsG mRNA target. Mutations that disrupt binding of a chaperone protein, Hfq, and are distal to the mRNA annealing region still decrease the rate of target association, kon, and increase the dissociation rate, koff, showing that Hfq directly facilitates sRNA-mRNA annealing in vivo. Single base-pair mismatches in the annealing region reduce kon by 24-31% and increase koff by 14-25%, extending the time it takes to find and destroy the target by about a third. The effects of disrupting contiguous base-pairing are much more modest than that expected from thermodynamics, suggesting that Hfq buffers base-pair disruptions." @default.
• W3129129219 created "2021-02-15" @default.
• W3129129219 creator A5004867963 @default.
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• W3129129219 creator A5087832374 @default.
• W3129129219 creator A5090048523 @default.
• W3129129219 date "2021-02-08" @default.
• W3129129219 modified "2023-10-16" @default.
• W3129129219 title "Effects of individual base-pairs on in vivo target search and destruction kinetics of bacterial small RNA" @default.
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• W3129129219 doi "https://doi.org/10.1038/s41467-021-21144-0" @default.
• W3129129219 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7870926" @default.
• W3129129219 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33558533" @default.
• W3129129219 hasPublicationYear "2021" @default.
• W3129129219 type Work @default.
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