Matches in SemOpenAlex for { <https://semopenalex.org/work/W3129385810> ?p ?o ?g. }
- W3129385810 abstract "Abstract Red alga dulse contains xylan with β(1→3)/β(1→4) linkages. We previously prepared xylooligosaccharides (XOSs) from dulse xylan; however, the product contained many d -xylose residues and fewer XOSs with β(1→3) linkages. To improve the efficiency of XOS production, we prepared two recombinant endoxylanases from Streptomyces thermogriseus (StXyl10 and StXyl11). Comparing the k cat / K m values for dulse xylan, this value from StXyl10 was approximately two times higher than that from StXyl11. We then determined the suitable conditions for XOS production. As a result, dulse XOS was prepared by the successive hydrolysis of 10 mg/mL dulse xylan by 0.5 μg/mL StXyl10 for 4 h at 50 °C and then 2.0 μg/mL StXyl11 for 36 h at 60 °C. Xylan was converted into 95.8% XOS, including 59.7% XOS with a β(1→3) linkage and 0.97% d -xylose. Our study provides useful information for the production of XOSs with β(1→3) linkages." @default.
- W3129385810 created "2021-03-01" @default.
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- W3129385810 date "2021-05-12" @default.
- W3129385810 modified "2023-09-30" @default.
- W3129385810 title "Preparation of β(1→3)/β(1→4) xylooligosaccharides from red alga dulse by two xylanases from Streptomyces thermogriseus" @default.
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- W3129385810 doi "https://doi.org/10.1186/s40643-021-00390-6" @default.
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