Matches in SemOpenAlex for { <https://semopenalex.org/work/W3129411673> ?p ?o ?g. }
- W3129411673 abstract "Cargo proteins of the type IX secretion system (T9SS) in human pathogens from the Bacteroidetes phylum invariably possess a conserved C-terminal domain (CTD) that functions as a signal for outer membrane (OM) translocation. In Porphyromonas gingivalis, the CTD of cargos is cleaved off after translocation, and anionic lipopolysaccharide (A-LPS) is attached. This transpeptidase reaction anchors secreted proteins to the OM. PorZ, a cell surface-associated protein, is an essential component of the T9SS whose function was previously unknown. We recently solved the crystal structure of PorZ and found that it consists of two β-propeller moieties, followed by a CTD. In this study, we performed structure-based modeling, suggesting that PorZ is a carbohydrate-binding protein. Indeed, we found that recombinant PorZ specifically binds A-LPS in vitro Binding was blocked by monoclonal antibodies that specifically react with a phosphorylated branched mannan in the anionic polysaccharide (A-PS) component of A-LPS, but not with the core oligosaccharide or the lipid A endotoxin. Examination of A-LPS derived from a cohort of mutants producing various truncations of A-PS confirmed that the phosphorylated branched mannan is indeed the PorZ ligand. Moreover, purified recombinant PorZ interacted with the PorU sortase in an A-LPS-dependent manner. This interaction on the cell surface is crucial for the function of the attachment complex composed of PorU, PorZ, and the integral OM β-barrel proteins PorV and PorQ, which is involved in posttranslational modification and retention of T9SS cargos on the bacterial surface.IMPORTANCE Bacteria have evolved multiple systems to transport effector proteins to their surface or into the surrounding milieu. These proteins have a wide range of functions, including attachment, motility, nutrient acquisition, and toxicity in the host. Porphyromonas gingivalis, the human pathogen responsible for severe gum diseases (periodontitis), uses a recently characterized type IX secretion system (T9SS) to translocate and anchor secreted virulence effectors to the cell surface. Anchorage is facilitated by sortase, an enzyme that covalently attaches T9SS cargo proteins to a unique anionic lipopolysaccharide (A-LPS) moiety of P. gingivalis Here, we show that the T9SS component PorZ interacts with sortase and specifically binds A-LPS. Binding is mediated by a phosphorylated branched mannan repeat in A-LPS polysaccharide. A-LPS-bound PorZ interacts with sortase with significantly higher affinity, facilitating modification of cargo proteins by the cell surface attachment complex of the T9SS." @default.
- W3129411673 created "2021-03-01" @default.
- W3129411673 creator A5001212903 @default.
- W3129411673 creator A5004101500 @default.
- W3129411673 creator A5004238852 @default.
- W3129411673 creator A5011071986 @default.
- W3129411673 creator A5023079125 @default.
- W3129411673 creator A5034381369 @default.
- W3129411673 creator A5046156279 @default.
- W3129411673 creator A5075210706 @default.
- W3129411673 creator A5076361578 @default.
- W3129411673 creator A5078249243 @default.
- W3129411673 creator A5082032451 @default.
- W3129411673 creator A5084491239 @default.
- W3129411673 creator A5087231208 @default.
- W3129411673 creator A5089350107 @default.
- W3129411673 date "2021-02-23" @default.
- W3129411673 modified "2023-10-14" @default.
- W3129411673 title "PorZ, an Essential Component of the Type IX Secretion System of <i>Porphyromonas gingivalis</i> , Delivers Anionic Lipopolysaccharide to the PorU Sortase for Transpeptidase Processing of T9SS Cargo Proteins" @default.
- W3129411673 cites W1836157892 @default.
- W3129411673 cites W1854259035 @default.
- W3129411673 cites W1895718854 @default.
- W3129411673 cites W1963828821 @default.
- W3129411673 cites W1964516944 @default.
- W3129411673 cites W1979101811 @default.
- W3129411673 cites W1990974422 @default.
- W3129411673 cites W1994774658 @default.
- W3129411673 cites W1999292622 @default.
- W3129411673 cites W2005019603 @default.
- W3129411673 cites W2028911325 @default.
- W3129411673 cites W2047960410 @default.
- W3129411673 cites W2064903683 @default.
- W3129411673 cites W2113541275 @default.
- W3129411673 cites W2113552067 @default.
- W3129411673 cites W2115137085 @default.
- W3129411673 cites W2115851064 @default.
- W3129411673 cites W2122151275 @default.
- W3129411673 cites W2124310880 @default.
- W3129411673 cites W2132663081 @default.
- W3129411673 cites W2157107389 @default.
- W3129411673 cites W2158270251 @default.
- W3129411673 cites W2159326694 @default.
- W3129411673 cites W2162148650 @default.
- W3129411673 cites W2168974130 @default.
- W3129411673 cites W2169722961 @default.
- W3129411673 cites W2170489814 @default.
- W3129411673 cites W2306642126 @default.
- W3129411673 cites W2310063671 @default.
- W3129411673 cites W2314285321 @default.
- W3129411673 cites W2317445083 @default.
- W3129411673 cites W2497844277 @default.
- W3129411673 cites W2529479209 @default.
- W3129411673 cites W2551556758 @default.
- W3129411673 cites W2575778933 @default.
- W3129411673 cites W2603411688 @default.
- W3129411673 cites W2619363404 @default.
- W3129411673 cites W2736180350 @default.
- W3129411673 cites W2745725076 @default.
- W3129411673 cites W2747791272 @default.
- W3129411673 cites W2786447801 @default.
- W3129411673 cites W2912555658 @default.
- W3129411673 cites W2938574745 @default.
- W3129411673 cites W3023440517 @default.
- W3129411673 cites W3023628373 @default.
- W3129411673 cites W3025156016 @default.
- W3129411673 cites W4211223324 @default.
- W3129411673 cites W4231492118 @default.
- W3129411673 cites W4234317413 @default.
- W3129411673 cites W4242679254 @default.
- W3129411673 doi "https://doi.org/10.1128/mbio.02262-20" @default.
- W3129411673 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8545088" @default.
- W3129411673 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33622730" @default.
- W3129411673 hasPublicationYear "2021" @default.
- W3129411673 type Work @default.
- W3129411673 sameAs 3129411673 @default.
- W3129411673 citedByCount "14" @default.
- W3129411673 countsByYear W31294116732021 @default.
- W3129411673 countsByYear W31294116732022 @default.
- W3129411673 countsByYear W31294116732023 @default.
- W3129411673 crossrefType "journal-article" @default.
- W3129411673 hasAuthorship W3129411673A5001212903 @default.
- W3129411673 hasAuthorship W3129411673A5004101500 @default.
- W3129411673 hasAuthorship W3129411673A5004238852 @default.
- W3129411673 hasAuthorship W3129411673A5011071986 @default.
- W3129411673 hasAuthorship W3129411673A5023079125 @default.
- W3129411673 hasAuthorship W3129411673A5034381369 @default.
- W3129411673 hasAuthorship W3129411673A5046156279 @default.
- W3129411673 hasAuthorship W3129411673A5075210706 @default.
- W3129411673 hasAuthorship W3129411673A5076361578 @default.
- W3129411673 hasAuthorship W3129411673A5078249243 @default.
- W3129411673 hasAuthorship W3129411673A5082032451 @default.
- W3129411673 hasAuthorship W3129411673A5084491239 @default.
- W3129411673 hasAuthorship W3129411673A5087231208 @default.
- W3129411673 hasAuthorship W3129411673A5089350107 @default.
- W3129411673 hasBestOaLocation W31294116733 @default.
- W3129411673 hasConcept C100817775 @default.
- W3129411673 hasConcept C104317684 @default.
- W3129411673 hasConcept C146587185 @default.
- W3129411673 hasConcept C165175003 @default.
- W3129411673 hasConcept C185592680 @default.