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- W3129558359 abstract "Abstract 3D single molecule localization microscopy (SMLM) is an emerging superresolution method for structural cell biology, as it allows probing precise positions of proteins in cellular structures. In supercritical angle localization microscopy (SALM), z-positions of single fluorophores are extracted from the intensity of supercritical angle fluorescence, which strongly depends on their distance to the coverslip. Here, we realize the full potential of SALM and improve its z-resolution by more than four-fold compared to the state-of-the-art by directly splitting supercritical and undercritical emission, using an ultra-high NA objective, and applying fitting routines to extract precise intensities of single emitters. We demonstrate nanometer isotropic localization precision on DNA origami structures, and on clathrin coated vesicles and microtubules in cells, illustrating the potential of SALM for cell biology." @default.
- W3129558359 created "2021-03-01" @default.
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- W3129558359 date "2021-02-19" @default.
- W3129558359 modified "2023-09-27" @default.
- W3129558359 title "Direct supercritical angle localization microscopy for nanometer 3D superresolution" @default.
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- W3129558359 doi "https://doi.org/10.1038/s41467-021-21333-x" @default.
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