FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3129744038> ?p ?o ?g. }

• W3129744038 endingPage "108812" @default.
• W3129744038 startingPage "108812" @default.
• W3129744038 abstract "Vitamin D status is routinely assessed by measuring circulating concentrations of 25-hydroxyvitamin D (25OHD2 or 25OHD3). However as deconjugation is not routinely incorporated into sample treatment prior to analysis, conjugated forms of 25OHD (particularly the more abundant 25OHD3) are often not considered in determining serum concentrations of total 25OHD. Two major circulating conjugated forms of 25OHD3 are 25-hydroxyvitamin D3-3-sulfate (25OHD3-S) and 25-hydroxyvitamin D3-3-glucuronide (25OHD3-G). Incorporating these two conjugated metabolites into the measurement of vitamin D status could improve our understanding of vitamin D status in health, particularly if there are changes in sulfation and glucuronidation activities. The aim of this study was to develop a liquid chromatography tandem-mass spectrometry (LC-MS/MS) targeted method for measurement of 25OHD3-S and 25OHD3-G in serum to enable comparisons with circulating levels of the free 25OHD3 form. We developed and validated a new LC-MS/MS method that measured both 25OHD3-S and 25OHD3-G following a solid phase extraction sample preparation method. Partial separation of analytes by LC, and the separation of analytes by the optimized multiple reaction monitoring transitions enabled the quantitation of both 25OHD3-S and 25OHD3-G in the single method. Serum concentrations of 25OHD3-S (24.7 ± 11.8 ng/mL) and 25OHD3-G (2.4 ± 1.2 ng/mL) were shown to be a significant proportion of circulating vitamin D metabolites in healthy donor serums. These levels of 25OHD3-S and 25OHD3-G closely associated with 25OHD3 concentrations, r = 0.728, p = 0.001 and r = 0.632, p = 0.006 respectively. However in serum from pregnant women and non-pregnant women with polycystic ovary syndrome (PCOS) significant differences in the ratios between conjugated and free 25OHD3 were observed between pregnancy groups (25OHD3/25OHD3-S and 25OHD3/25OHD3-G p < 0.001), and between healthy and PCOS subjects (25OHD3/25OHD3-G p < 0.050). Development of this novel high-throughput LC-MS/MS method indicates that 25OHD3-S and 25OHD3-G are substantial components of circulating vitamin D metabolites. The concentrations of these metabolites relative to conventional 25OHD3 may vary in different physiological and pathophysiological settings, and may therefore play an unrecognized but important role in the actions of vitamin D." @default.
• W3129744038 created "2021-03-01" @default.
• W3129744038 creator A5010869831 @default.
• W3129744038 creator A5027548347 @default.
• W3129744038 creator A5030645080 @default.
• W3129744038 creator A5031152388 @default.
• W3129744038 creator A5046060573 @default.
• W3129744038 creator A5055574817 @default.
• W3129744038 creator A5073724307 @default.
• W3129744038 date "2021-05-01" @default.
• W3129744038 modified "2023-10-02" @default.
• W3129744038 title "Development of a LC-MS/MS method to measure serum 3-sulfate and 3-glucuronide 25-hydroxyvitamin D3 metabolites; comparisons to unconjugated 25OHD in pregnancy and polycystic ovary syndrome" @default.
• W3129744038 cites W1657182757 @default.
• W3129744038 cites W1964155369 @default.
• W3129744038 cites W1965505065 @default.
• W3129744038 cites W1967662402 @default.
• W3129744038 cites W1971998725 @default.
• W3129744038 cites W1976776496 @default.
• W3129744038 cites W2011725731 @default.
• W3129744038 cites W2036640577 @default.
• W3129744038 cites W2038469606 @default.
• W3129744038 cites W2041787000 @default.
• W3129744038 cites W2050616362 @default.
• W3129744038 cites W2053491264 @default.
• W3129744038 cites W2065321690 @default.
• W3129744038 cites W2070338045 @default.
• W3129744038 cites W2071022522 @default.
• W3129744038 cites W2080260619 @default.
• W3129744038 cites W2080844332 @default.
• W3129744038 cites W2088098532 @default.
• W3129744038 cites W2107905356 @default.
• W3129744038 cites W2186218348 @default.
• W3129744038 cites W2253762472 @default.
• W3129744038 cites W2274269529 @default.
• W3129744038 cites W2511046668 @default.
• W3129744038 cites W2557976244 @default.
• W3129744038 cites W2566085762 @default.
• W3129744038 cites W2623598848 @default.
• W3129744038 cites W2684997942 @default.
• W3129744038 cites W2738784218 @default.
• W3129744038 cites W2787724776 @default.
• W3129744038 cites W2790887296 @default.
• W3129744038 cites W2886348975 @default.
• W3129744038 cites W2922086857 @default.
• W3129744038 cites W2923674502 @default.
• W3129744038 cites W2963539608 @default.
• W3129744038 cites W3012276600 @default.
• W3129744038 cites W3040975274 @default.
• W3129744038 doi "https://doi.org/10.1016/j.steroids.2021.108812" @default.
• W3129744038 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33636208" @default.
• W3129744038 hasPublicationYear "2021" @default.
• W3129744038 type Work @default.
• W3129744038 sameAs 3129744038 @default.
• W3129744038 citedByCount "4" @default.
• W3129744038 countsByYear W31297440382021 @default.
• W3129744038 crossrefType "journal-article" @default.
• W3129744038 hasAuthorship W3129744038A5010869831 @default.
• W3129744038 hasAuthorship W3129744038A5027548347 @default.
• W3129744038 hasAuthorship W3129744038A5030645080 @default.
• W3129744038 hasAuthorship W3129744038A5031152388 @default.
• W3129744038 hasAuthorship W3129744038A5046060573 @default.
• W3129744038 hasAuthorship W3129744038A5055574817 @default.
• W3129744038 hasAuthorship W3129744038A5073724307 @default.
• W3129744038 hasBestOaLocation W31297440382 @default.
• W3129744038 hasConcept C185592680 @default.
• W3129744038 hasConcept C43617362 @default.
• W3129744038 hasConceptScore W3129744038C185592680 @default.
• W3129744038 hasConceptScore W3129744038C43617362 @default.
• W3129744038 hasLocation W31297440381 @default.
• W3129744038 hasLocation W31297440382 @default.
• W3129744038 hasLocation W31297440383 @default.
• W3129744038 hasLocation W31297440384 @default.
• W3129744038 hasOpenAccess W3129744038 @default.
• W3129744038 hasPrimaryLocation W31297440381 @default.
• W3129744038 hasRelatedWork W1531601525 @default.
• W3129744038 hasRelatedWork W2319480705 @default.
• W3129744038 hasRelatedWork W2384464875 @default.
• W3129744038 hasRelatedWork W2398689458 @default.
• W3129744038 hasRelatedWork W2606230654 @default.
• W3129744038 hasRelatedWork W2607424097 @default.
• W3129744038 hasRelatedWork W2748952813 @default.
• W3129744038 hasRelatedWork W2899084033 @default.
• W3129744038 hasRelatedWork W2948807893 @default.
• W3129744038 hasRelatedWork W2778153218 @default.
• W3129744038 hasVolume "169" @default.
• W3129744038 isParatext "false" @default.
• W3129744038 isRetracted "false" @default.
• W3129744038 magId "3129744038" @default.
• W3129744038 workType "article" @default.