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• W3129988892 abstract "Candidiasis is a fungal infection leading to significant morbidity and mortality inimmunocompromised patients. Aureobasidin (AbA), a cyclic depsipeptide antifungaldrug, has been reported to be effective against Candida albicans but is yet to be used fortherapy. Although AbA is presumed to target the fungal sphingolipid biosynthesis, theexact mechanism of action has yet to be defined. As a prerequisite to study themechanism of action of this antifungal drug, this study investigated changes in theproteome of planktonic and biofilm cultures of C. albicans upon exposure to AbA. Inthis study, the whole cell extracts of biofilm and planktonic cultures of C.albicans strain SC5314 pre- and post-exposure to AbA were subjected to liquidchromatography mass spectrometry (LC-MS/MS) analysis. Using sequence homologysearch tool (SPIDER) of PEAKS software (ver. 7.5), proteins were identified based onthe setting of at least one unique peptide matched and a significance (-10lgP) value of>20. The proteome prior to AbA exposure demonstrated a total of 203 and 533 proteinsfrom the planktonic and biofilm cultures, respectively, with a false discovery rate of≤1%. The biological process, molecular function, and subcellular localization of biofilmand planktonic proteins were annotated using Go Slim Mapper of the online CandidaGenome Database. Classification of biological processes demonstrated greaterdifferences in the number of biofilm proteins associated with the regulation ofbiological processes (n=65), organelle organization (n=57), transport (n=55), stressresponse (n=47), filamentous growth (n=30), chemical response (n=30), lipidmetabolism (n=23) and carbohydrate metabolism (n=20) compared to planktonicculture. As for molecular function, no major difference was observed in both cultures, ivwith approximately 10-20% of the proteins are associated with hydrolase, transferaseand protein binding activities. On subcellular localization, 68.7 and 66.4% proteins ofplanktonic and biofilm cultures were predicted to be localized in cytoplasm. A total of293 and 374 proteins were annotated in the AbA-treated planktonic and biofilm cultureswith sub-inhinbitory AbA concentration (1 µg/ml) for 2.5 hours. A lower percentage ofproteins annotated for organelle organization in the AbA-treated planktonic culture(18.1%) as compared to the dimethyl sulfoxide (DMSO)-treated planktonic culture(23.2%). There was a 5% increase in the proteins annotated for translation in AbAtreated planktonic culture. AbA-treated biofilm culture has shown reduction in thepercentages of proteins annotated for vesicle-mediated transport (from 8.4 to 5.3%) andfilamentous growth (15.8 to 12.8%), and an increase in the percentage of proteinsannotated for carbohydrate metabolic process (8.2 to 12.0%). The regulation of cellularamide metabolic process and actin cytoskeleton organization are two biologicalprocesses shared by AbA-treated planktonic and biofilm cultures. This study alsoreviewed some C. albicans proteins which are annotated for pathogenesis, biofilmformation, lipid metabolic process and filamentous growth in response to AbA. Furtherexploration of the drug-affected proteins may aid in the search for potential drug targetin the treatment of candidiasis." @default.
• W3129988892 created "2021-03-01" @default.
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• W3129988892 date "2019-01-01" @default.
• W3129988892 modified "2023-09-23" @default.
• W3129988892 title "Protein profiling of Candida albicans planktonic and biofilm cultures upon exposure to Aureobasidin / Komathy Munusamy" @default.
• W3129988892 hasPublicationYear "2019" @default.
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