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- W3130202222 abstract "The small intestinal epithelium is the primary route of infection for many protozoan parasites. Understanding the mechanisms of infection, however, has been hindered due to the lack of appropriate models that recapitulate the complexity of the intestinal epithelium. Here, we describe an in vitro platform using stem cell-derived intestinal organoids established for four species that are important hosts of Apicomplexa and other protozoa in a zoonotic context: human, mouse, pig and chicken. The focus was set to create organoid-derived monolayers (ODMs) using the transwell system amenable for infection studies, and we provide straightforward guidelines for their generation and differentiation from organ-derived intestinal crypts. To this end, we reduced medium variations to an absolute minimum, allowing generation and differentiation of three-dimensional organoids for all four species and the subsequent generation of ODMs. Quantitative RT-PCR, immunolabeling with antibodies against marker proteins as well as transepithelial-electrical resistance (TEER) measurements were used to characterize ODM’s integrity and functional state. These experiments show an overall uniform generation of monolayers suitable for Toxoplasma gondii infection, although robustness in terms of generation of stable TEER levels and cell differentiation status varies from species to species. Murine duodenal ODMs were then infected with T. gondii and/or Giardia duodenalis , two parasites that temporarily co-inhabit the intestinal niche but have not been studied previously in cellular co-infection models. T. gondii alone did not alter TEER values, integrity and transcriptional abundance of tight junction components. In contrast, in G. duodenalis -infected ODMs all these parameters were altered and T. gondii had no apparent influence on the G. duodenalis -triggered phenotype. In conclusion, we provide robust protocols for the generation, differentiation and characterization of intestinal organoids and ODMs from four species. We show their applications for comparative studies on parasite-host interactions during the early phase of a T. gondii infection but also its use for co-infections with other relevant intestinal protozoans." @default.
- W3130202222 created "2021-03-01" @default.
- W3130202222 creator A5011008136 @default.
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- W3130202222 creator A5042349359 @default.
- W3130202222 creator A5046256280 @default.
- W3130202222 creator A5073917322 @default.
- W3130202222 date "2021-02-22" @default.
- W3130202222 modified "2023-10-10" @default.
- W3130202222 title "Harmonization of Protocols for Multi-Species Organoid Platforms to Study the Intestinal Biology of Toxoplasma gondii and Other Protozoan Infections" @default.
- W3130202222 cites W129082737 @default.
- W3130202222 cites W1964458196 @default.
- W3130202222 cites W1965157206 @default.
- W3130202222 cites W1983785927 @default.
- W3130202222 cites W1991236015 @default.
- W3130202222 cites W1996936063 @default.
- W3130202222 cites W2002513358 @default.
- W3130202222 cites W2002764344 @default.
- W3130202222 cites W2022302468 @default.
- W3130202222 cites W2025574003 @default.
- W3130202222 cites W2030787774 @default.
- W3130202222 cites W2032652559 @default.
- W3130202222 cites W2042045396 @default.
- W3130202222 cites W2052891178 @default.
- W3130202222 cites W2058237201 @default.
- W3130202222 cites W2063858974 @default.
- W3130202222 cites W2099540110 @default.
- W3130202222 cites W2101594125 @default.
- W3130202222 cites W2102152513 @default.
- W3130202222 cites W2104186584 @default.
- W3130202222 cites W2106125311 @default.
- W3130202222 cites W2113339994 @default.
- W3130202222 cites W2117992130 @default.
- W3130202222 cites W2121900881 @default.
- W3130202222 cites W2136443727 @default.
- W3130202222 cites W2138224451 @default.
- W3130202222 cites W2139419405 @default.
- W3130202222 cites W2144509281 @default.
- W3130202222 cites W2146276688 @default.
- W3130202222 cites W2147832388 @default.
- W3130202222 cites W2152369313 @default.
- W3130202222 cites W2154607802 @default.
- W3130202222 cites W2157010562 @default.
- W3130202222 cites W2184405267 @default.
- W3130202222 cites W2188611891 @default.
- W3130202222 cites W2195721971 @default.
- W3130202222 cites W2234343426 @default.
- W3130202222 cites W2441337908 @default.
- W3130202222 cites W2465742979 @default.
- W3130202222 cites W2562023464 @default.
- W3130202222 cites W2598201562 @default.
- W3130202222 cites W2603154743 @default.
- W3130202222 cites W2606736166 @default.
- W3130202222 cites W2607153248 @default.
- W3130202222 cites W2620385663 @default.
- W3130202222 cites W2708922922 @default.
- W3130202222 cites W2744377069 @default.
- W3130202222 cites W2756509223 @default.
- W3130202222 cites W2769549586 @default.
- W3130202222 cites W2775793257 @default.
- W3130202222 cites W2789342073 @default.
- W3130202222 cites W2791816601 @default.
- W3130202222 cites W2799401309 @default.
- W3130202222 cites W2802981078 @default.
- W3130202222 cites W2803107917 @default.
- W3130202222 cites W2804673796 @default.
- W3130202222 cites W2809107133 @default.
- W3130202222 cites W2884529146 @default.
- W3130202222 cites W2894066055 @default.
- W3130202222 cites W2917423992 @default.
- W3130202222 cites W2919485321 @default.
- W3130202222 cites W2923393751 @default.
- W3130202222 cites W2932164846 @default.
- W3130202222 cites W2941080670 @default.
- W3130202222 cites W2946321446 @default.
- W3130202222 cites W2951791227 @default.
- W3130202222 cites W2964955072 @default.
- W3130202222 cites W2969463432 @default.
- W3130202222 cites W2970307024 @default.
- W3130202222 cites W2995456137 @default.
- W3130202222 cites W2996860650 @default.
- W3130202222 cites W3002852877 @default.
- W3130202222 cites W3003721121 @default.
- W3130202222 cites W3008356216 @default.
- W3130202222 cites W3016594809 @default.
- W3130202222 cites W3036643370 @default.
- W3130202222 cites W3038518028 @default.
- W3130202222 cites W3040770894 @default.
- W3130202222 cites W3047626467 @default.
- W3130202222 cites W3093971243 @default.
- W3130202222 cites W4211140781 @default.
- W3130202222 cites W4211229759 @default.
- W3130202222 doi "https://doi.org/10.3389/fcimb.2020.610368" @default.
- W3130202222 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7937733" @default.
- W3130202222 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33692963" @default.
- W3130202222 hasPublicationYear "2021" @default.
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