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- W3130361567 abstract "Okanagan Specialty Fruits（OSF）公司所研發的基因改造蘋果，產品名為北極（Arctic）蘋果，其基改特性為抗褐變，利用RNA干擾（RNA interference）的方式抑制蘋果多酚氧化酶（polyphenol oxidase, PPO）的表現，降低PPO催化蘋果氧化形成褐色產物。目前北極金冠蘋果GD743和北極翠玉蘋果GS784已獲得美國及加拿大核准上市，主要以新鮮袋裝切片和蘋果乾的商品形式在美國販售，由於OSF公司的北極蘋果尚未向我國申請基因改造食品查驗登記，北極蘋果在台灣仍屬於未核准的基因改造食品，為了避免國際非同步許可所造成的食安議題，本研究開發基因改造蘋果PCR及real-time PCR檢驗方法以因應檢驗所需。針對pSR7基因與NOS終結子區域設計的構造特異性（construct-specific）PCR及real-time PCR檢驗方法可檢測北極蘋果，TFDA現有CaMV 35S與NOS定性方法也適用於北極蘋果的篩檢。進一步分析金冠北極蘋果GD743的轉殖基因及其跨接區域，除了確認北極金冠蘋果GD743的轉殖基因序列全長，亦找到2個不同的5’端轉殖基因片段跨接蘋果基因體區域源自GD743中2個不同的殖入片段拷貝，本研究已確認2個不同片段中的1個拷貝轉殖基因片段位於蘋果chromosome 14具特異性可做為基改蘋果檢測，並進一步確認3’端跨接蘋果chromosome 14的基因序列，運用解析出5’端及3’端跨接蘋果chromosome 14的核酸序列，建立北極金冠蘋果GD743轉殖品項特異性（event-specific） real-time PCR檢驗方法。" @default.
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- W3130361567 date "2019-12-01" @default.
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- W3130361567 title "Development of Real-Time PCR Detection Methods on Genetically Modified Apple" @default.
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