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- W3130371291 abstract "Abstract Nowadays, scientists develop multiple methods to control pests, like chemical control as the insecticides and biological control as the Sterile Insect Technique (SIT) to release infertile males produced by irradiation. This technique is complemented with research in the medfly’s (Ceratitis capitata) microbiota, this approach is based on the identification of molecular mechanisms from three Klebsiella strains using the reconstruction of metabolic pathways technique. We focus on the reconstruction of metabolic pathways involved in nitrogen metabolization because the bacteria are in charge of metabolizing the nitrogen compounds and make available for the medfly. We propose a pipeline to process the genome sequences the bacteria, reconstruct metabolic pathways and identify possible symbiont molecular mechanisms. The result of processing the biological data were ten pathways, six pathways produce L-Glutamate as final product, three pathways with ammonia product and one pathway was discarded because it was an internal pathway and don’t generate a metabolic product. Those pathways corresponding to L-Glutamate and ammonia metabolic products are part of nitrogen metabolism on Klebsiella , which shows that this metabolic process is one of the molecular mechanisms involved in symbiosis process between Klebsiella and the medfly. Finally, by the results of the sequences analysis, in the medfly’s metamorphose the L-Glutamate are used in the synthesis of new proteins and the production of energy. The final consideration suggest that the pipeline propose can be used as first step to identify molecular mechanisms that can improve the production of industrial the medfly." @default.
- W3130371291 created "2021-03-01" @default.
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- W3130371291 date "2021-02-14" @default.
- W3130371291 modified "2023-09-26" @default.
- W3130371291 title "A computational approach to identify possible symbiotic mechanisms between Klebsiella and the Mediterranean fly (Ceratitis capitata)" @default.
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- W3130371291 doi "https://doi.org/10.1101/2021.02.13.431071" @default.
- W3130371291 hasPublicationYear "2021" @default.
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