Matches in SemOpenAlex for { <https://semopenalex.org/work/W3130675997> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W3130675997 endingPage "1258" @default.
- W3130675997 startingPage "1253" @default.
- W3130675997 abstract "The present work studied an acclimation method for phosphorus accumulating organisms (PAOs) with a high content of acetone in culture solutions to develop microbial-based enzyme sensors for highly hydrophobic organophosphorus (OP) pesticides. Through three steps of cultivation and acclimation, only rod-shaped bacteria survived among the various PAOs. The extracellular enzymes released from the acclimated PAOs were salted out by using ammonium sulfate, then purified by a dialysis membrane and a DEAE-Sepharose FF anion exchange column. Two enzyme components were successfully separated-both of which showed hydrolase activity on disodium p-nitrophenyl phosphate (enzyme I, 1.57 μmol/(min·μg); enzyme II, 0.88 μmol/(min·μg) at 45°C). Further, SDS-PAGE gel electrophoresis results showed that the molecular weights of enzymes I and II were about 15.11 and 11.98 kDa, respectively. On this basis, the applicability of the enzyme in hydrophobic OP biosensors was demonstrated." @default.
- W3130675997 created "2021-03-01" @default.
- W3130675997 creator A5009012762 @default.
- W3130675997 creator A5012047470 @default.
- W3130675997 creator A5025668728 @default.
- W3130675997 creator A5035721882 @default.
- W3130675997 creator A5079311802 @default.
- W3130675997 creator A5085554195 @default.
- W3130675997 date "2021-02-19" @default.
- W3130675997 modified "2023-10-16" @default.
- W3130675997 title "Isolation and Purification of Organophosphorus Hydrolases Secreted from Acetone-acclimated Phosphorus Accumulating Organisms and Study of Their Properties for Hydrophobic Organophosphorus Sensor" @default.
- W3130675997 cites W1592741796 @default.
- W3130675997 cites W1919632508 @default.
- W3130675997 cites W1970399725 @default.
- W3130675997 cites W1993114356 @default.
- W3130675997 cites W1995630950 @default.
- W3130675997 cites W2002164148 @default.
- W3130675997 cites W2005477498 @default.
- W3130675997 cites W2011273580 @default.
- W3130675997 cites W2014978522 @default.
- W3130675997 cites W2020226602 @default.
- W3130675997 cites W2020475948 @default.
- W3130675997 cites W2030334072 @default.
- W3130675997 cites W2030925785 @default.
- W3130675997 cites W2038343400 @default.
- W3130675997 cites W2038890168 @default.
- W3130675997 cites W2076818958 @default.
- W3130675997 cites W2134116635 @default.
- W3130675997 cites W2144455554 @default.
- W3130675997 cites W2164358316 @default.
- W3130675997 cites W2170466099 @default.
- W3130675997 cites W2288599908 @default.
- W3130675997 cites W2412027839 @default.
- W3130675997 cites W2465675992 @default.
- W3130675997 cites W2533694442 @default.
- W3130675997 cites W2902560660 @default.
- W3130675997 cites W2957037272 @default.
- W3130675997 cites W2963869615 @default.
- W3130675997 cites W2999765689 @default.
- W3130675997 cites W3016347510 @default.
- W3130675997 cites W3016515039 @default.
- W3130675997 cites W4251528927 @default.
- W3130675997 doi "https://doi.org/10.2116/analsci.21p001" @default.
- W3130675997 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33612559" @default.
- W3130675997 hasPublicationYear "2021" @default.
- W3130675997 type Work @default.
- W3130675997 sameAs 3130675997 @default.
- W3130675997 citedByCount "0" @default.
- W3130675997 crossrefType "journal-article" @default.
- W3130675997 hasAuthorship W3130675997A5009012762 @default.
- W3130675997 hasAuthorship W3130675997A5012047470 @default.
- W3130675997 hasAuthorship W3130675997A5025668728 @default.
- W3130675997 hasAuthorship W3130675997A5035721882 @default.
- W3130675997 hasAuthorship W3130675997A5079311802 @default.
- W3130675997 hasAuthorship W3130675997A5085554195 @default.
- W3130675997 hasBestOaLocation W31306759971 @default.
- W3130675997 hasConcept C116084860 @default.
- W3130675997 hasConcept C178790620 @default.
- W3130675997 hasConcept C181199279 @default.
- W3130675997 hasConcept C185592680 @default.
- W3130675997 hasConcept C2779870022 @default.
- W3130675997 hasConcept C2781213060 @default.
- W3130675997 hasConcept C28212737 @default.
- W3130675997 hasConcept C43617362 @default.
- W3130675997 hasConcept C510538283 @default.
- W3130675997 hasConcept C55493867 @default.
- W3130675997 hasConceptScore W3130675997C116084860 @default.
- W3130675997 hasConceptScore W3130675997C178790620 @default.
- W3130675997 hasConceptScore W3130675997C181199279 @default.
- W3130675997 hasConceptScore W3130675997C185592680 @default.
- W3130675997 hasConceptScore W3130675997C2779870022 @default.
- W3130675997 hasConceptScore W3130675997C2781213060 @default.
- W3130675997 hasConceptScore W3130675997C28212737 @default.
- W3130675997 hasConceptScore W3130675997C43617362 @default.
- W3130675997 hasConceptScore W3130675997C510538283 @default.
- W3130675997 hasConceptScore W3130675997C55493867 @default.
- W3130675997 hasIssue "9" @default.
- W3130675997 hasLocation W31306759971 @default.
- W3130675997 hasLocation W31306759972 @default.
- W3130675997 hasOpenAccess W3130675997 @default.
- W3130675997 hasPrimaryLocation W31306759971 @default.
- W3130675997 hasRelatedWork W1665732417 @default.
- W3130675997 hasRelatedWork W1988259208 @default.
- W3130675997 hasRelatedWork W1988346978 @default.
- W3130675997 hasRelatedWork W2010372900 @default.
- W3130675997 hasRelatedWork W2326752735 @default.
- W3130675997 hasRelatedWork W2356650198 @default.
- W3130675997 hasRelatedWork W2370287921 @default.
- W3130675997 hasRelatedWork W2379318974 @default.
- W3130675997 hasRelatedWork W2384585446 @default.
- W3130675997 hasRelatedWork W412475053 @default.
- W3130675997 hasVolume "37" @default.
- W3130675997 isParatext "false" @default.
- W3130675997 isRetracted "false" @default.
- W3130675997 magId "3130675997" @default.
- W3130675997 workType "article" @default.