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- W3130883002 abstract "The biophysical properties of the plasma membrane of the filamentous fungus Neurospora crassa was characterized using fluorescence spectroscopy. We have taken advantage of a strategy relying on the combined use of different membrane probes and photophysical properties to monitor, in vivo, thebiophysical changes upon a drug challenge with staurosporine, a programmed cell death-inducing drug, in N. crassa asexual spores (conidia). An increase in the rigidity of highly ordered membrane domains was observed, possibly associated with the increment on the levels of sphingolipids with smaller headgroups, allowing a tighter packing of the lipid acyl chains. This stronger segregation of sphingolipids contributes to a decreased order in the remainder membrane regions. Thus, globally the membrane order remains essentially unchanged. in the conidial stage, N. crassa presents vestigial amounts of ergosterol. However, during transition to the mycelium stage, it increases dramatically. Taking advantage of this unique feature, we could establish that the amplitude-weighted mean fluorescence lifetime (τav) of di-4-ANEPPS, a probe sensitive to membrane polarity properties, correlates remarkably with the ergosterol/glycerophospholipid ratio. Along conidial germination, both departure from small values that further decrease and then increase abruptly upon starting the formation of the mycelium. The addition of staurosporine at 5-h growth prevents this marked increase of τav of di-4-ANEPPS, i.e., it seems that the drug inhibits mycelium formation. Hence, it was possible to establish clear relations between plasma membrane biophysical properties involving sphingolipids and sterols and drug response in vivo. Moreover, we developed a sensitive and specific spectroscopic method to follow ergosterol/glycerophospholipid ratio in N. crassa, an important biochemical parameter in biomembrane analysis." @default.
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- W3130883002 date "2021-02-01" @default.
- W3130883002 modified "2023-10-18" @default.
- W3130883002 title "Fungal Plasma Membrane Lipid Domains Alterations Upon Drug Exposure" @default.
- W3130883002 doi "https://doi.org/10.1016/j.bpj.2020.11.520" @default.
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