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- W3131738010 abstract "Abstract In order to better understand the mechanism of glycyrrhizic acid biosynthesis and explore important enzyme gene resources in Glycyrrhiza uralensis Fisch., we sequenced the transcriptome of the adventitious roots of G. uralensis treated by methyl jasmonate (MJ) and assembled the de novo sequence. 256503 unique transcripts with an average length of 898bp were produced. Transcriptome sequencing and data analysis showed that the key genes of glycyrrhizic acid biosynthesis changed significantly after MJ treatment. 2720 up-regulated genes and 3493 down regulated genes were found. In the process of oxidation and glycosylation of glycyrrhizic acid biosynthesis. A putative CYP450 gene (Cluster-30944.70498) is positively correlated with glycyrrhetinic acid. The glycosyltransferase gene (Cluster-30944.25725) is positively correlated with glycyrrhizic acid and glycyrrhetinic acid. In addition, we found an AP2-EREBP family transcription factor (Cluster-30944.55070). It had high amino acid sequence similarity with PgERF1. In Panax ginseng , PgERF1 was identified as promoting the biosynthesis of triterpenoid saponins. According to the correlation analysis of transcription factors, functional gene expression and component accumulation, we speculated that this transcription factor can positively regulate the expression of farnesyl diphosphate, squalene epoxide and glycosyltransferase (Cluster-30944.25725) genes and ultimately increase the content of glycyrrhizic acid." @default.
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- W3131738010 date "2021-02-16" @default.
- W3131738010 modified "2023-10-12" @default.
- W3131738010 title "Transcriptomic Analysis Reveals the Mechanism of Glycyrrhizic acid Biosynthesis in Glycyrrhiza Uralensis Fisch." @default.
- W3131738010 doi "https://doi.org/10.21203/rs.3.rs-231260/v1" @default.
- W3131738010 hasPublicationYear "2021" @default.
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