FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3131834668> ?p ?o ?g. }

• W3131834668 endingPage "114484" @default.
• W3131834668 startingPage "114484" @default.
• W3131834668 abstract "Many drugs are largely hydrophobic molecules; a transporter might conceivably insert these into the plasma membrane. At least 18 transporters from diverse families have been reported to transport the model compound estrone sulfate alias estrone-3-sulfate (E3S). Out of these, we recently examined SLC22A11 (OAT4). We concluded from a comparison of E3S and uric acid transport that SLC22A11 does not translocate E3S into the cytosol, but into the plasma membrane. Here we present a hyperosmolarity alias hypertonicity assay to differentiate transport mechanisms. Human transporters were expressed heterologously in 293 cells. Solute uptake into intact cells was measured by LC-MS. Addition of mannitol or sucrose led to rapid cell shrinkage, but cell viability after 60 min in hyperosmolar buffer was not impaired. A decrease in substrate accumulation with increasing osmolarity as observed here for several substrates and the transporters SLC22A11, ETT (SLC22A4), OCT2 (SLC22A2), OAT3 (SLC22A8), and MATE1 (SLC47A1) suggests regular substrate translocation into the cytosol. An increase as observed for E3S transport by SLC22A11, OAT3, MATE1, SLC22A9, and SLC10A6 implies insertion into the membrane. In marked contrast to the other E3S transporters, the bile acid transporter SLC10A1 (NTCP, Na+ taurocholate co-transporting polypeptide) showed a decrease in the accumulation of E3S in hyperosmolar buffer; the same was observed with taurocholic acid. Indeed, our data from several functional assays strongly suggest that the transport mechanism is identical for both substrates. Apparently, a unique transport mechanism has been established for SLC10A1 by evolution that ensures the transport of amphipathic, detergent-like molecules into the cytosol." @default.
• W3131834668 created "2021-03-01" @default.
• W3131834668 creator A5059373304 @default.
• W3131834668 creator A5064136219 @default.
• W3131834668 creator A5077888141 @default.
• W3131834668 date "2021-04-01" @default.
• W3131834668 modified "2023-10-16" @default.
• W3131834668 title "Hyperosmolarity stimulates transporter-mediated insertion of estrone sulfate into the plasma membrane, but inhibits the uptake by SLC10A1 (NTCP)" @default.
• W3131834668 cites W1176493451 @default.
• W3131834668 cites W1481845508 @default.
• W3131834668 cites W1536734151 @default.
• W3131834668 cites W1578075719 @default.
• W3131834668 cites W1580329462 @default.
• W3131834668 cites W1964471078 @default.
• W3131834668 cites W1978910625 @default.
• W3131834668 cites W1986115510 @default.
• W3131834668 cites W1989276141 @default.
• W3131834668 cites W2008305695 @default.
• W3131834668 cites W2021938691 @default.
• W3131834668 cites W2026179713 @default.
• W3131834668 cites W2030090374 @default.
• W3131834668 cites W2046507147 @default.
• W3131834668 cites W2056202184 @default.
• W3131834668 cites W2060416843 @default.
• W3131834668 cites W2068899415 @default.
• W3131834668 cites W2074176521 @default.
• W3131834668 cites W2080484884 @default.
• W3131834668 cites W2085047894 @default.
• W3131834668 cites W2088160623 @default.
• W3131834668 cites W2091427271 @default.
• W3131834668 cites W2092637029 @default.
• W3131834668 cites W2093726578 @default.
• W3131834668 cites W2095156917 @default.
• W3131834668 cites W2105517464 @default.
• W3131834668 cites W2120426460 @default.
• W3131834668 cites W2121335499 @default.
• W3131834668 cites W2124260053 @default.
• W3131834668 cites W2137661705 @default.
• W3131834668 cites W2138673183 @default.
• W3131834668 cites W2145861024 @default.
• W3131834668 cites W2150628192 @default.
• W3131834668 cites W2161384494 @default.
• W3131834668 cites W2163109525 @default.
• W3131834668 cites W2164978829 @default.
• W3131834668 cites W2216793345 @default.
• W3131834668 cites W2216866477 @default.
• W3131834668 cites W2526653747 @default.
• W3131834668 cites W2561410591 @default.
• W3131834668 cites W2619656708 @default.
• W3131834668 cites W2888670687 @default.
• W3131834668 cites W2914077343 @default.
• W3131834668 cites W2939302038 @default.
• W3131834668 cites W2954724391 @default.
• W3131834668 cites W2956505453 @default.
• W3131834668 cites W3093097225 @default.
• W3131834668 cites W3130483070 @default.
• W3131834668 doi "https://doi.org/10.1016/j.bcp.2021.114484" @default.
• W3131834668 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33617845" @default.
• W3131834668 hasPublicationYear "2021" @default.
• W3131834668 type Work @default.
• W3131834668 sameAs 3131834668 @default.
• W3131834668 citedByCount "2" @default.
• W3131834668 countsByYear W31318346682021 @default.
• W3131834668 countsByYear W31318346682022 @default.
• W3131834668 crossrefType "journal-article" @default.
• W3131834668 hasAuthorship W3131834668A5059373304 @default.
• W3131834668 hasAuthorship W3131834668A5064136219 @default.
• W3131834668 hasAuthorship W3131834668A5077888141 @default.
• W3131834668 hasBestOaLocation W31318346682 @default.
• W3131834668 hasConcept C104317684 @default.
• W3131834668 hasConcept C115335371 @default.
• W3131834668 hasConcept C12554922 @default.
• W3131834668 hasConcept C129900496 @default.
• W3131834668 hasConcept C149011108 @default.
• W3131834668 hasConcept C181199279 @default.
• W3131834668 hasConcept C185592680 @default.
• W3131834668 hasConcept C2778656385 @default.
• W3131834668 hasConcept C2779149875 @default.
• W3131834668 hasConcept C2779399885 @default.
• W3131834668 hasConcept C2780480987 @default.
• W3131834668 hasConcept C55493867 @default.
• W3131834668 hasConcept C71315377 @default.
• W3131834668 hasConcept C86803240 @default.
• W3131834668 hasConcept C98539663 @default.
• W3131834668 hasConceptScore W3131834668C104317684 @default.
• W3131834668 hasConceptScore W3131834668C115335371 @default.
• W3131834668 hasConceptScore W3131834668C12554922 @default.
• W3131834668 hasConceptScore W3131834668C129900496 @default.
• W3131834668 hasConceptScore W3131834668C149011108 @default.
• W3131834668 hasConceptScore W3131834668C181199279 @default.
• W3131834668 hasConceptScore W3131834668C185592680 @default.
• W3131834668 hasConceptScore W3131834668C2778656385 @default.
• W3131834668 hasConceptScore W3131834668C2779149875 @default.
• W3131834668 hasConceptScore W3131834668C2779399885 @default.
• W3131834668 hasConceptScore W3131834668C2780480987 @default.
• W3131834668 hasConceptScore W3131834668C55493867 @default.
• W3131834668 hasConceptScore W3131834668C71315377 @default.
• W3131834668 hasConceptScore W3131834668C86803240 @default.

• next