FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3132010090> ?p ?o ?g. }

• W3132010090 endingPage "237" @default.
• W3132010090 startingPage "226" @default.
• W3132010090 abstract "Oxidative stress plays an important role in the pathogenesis of osteoporosis and impaired bone formation. However, the mechanisms behind which oxidative stress represses bone formation remains unclear. TP53INP2, a target of the tumor suppressor p53, is ubiquitously expressed in various cell types including BMSCs and contributes to autophagosome formation by recruiting ubiquitinated substrates to autophagosomes for degradation. However, little is known about its function in BMSCs and its relation to osteoporosis. In this study, first, we verified that the expression of TP53INP2 was persistently decreased in BMSCs derived from osteoporosis patients and OVX mice, and that the antioxidant N-acetylcysteine could ameliorate this decreased TP53INP2 level in vitro. Second, we identified that the mRNA and protein levels of TP53INP2 decreased in BMSCs under H2O2 induced oxidative stress in a dose-dependent manner, with resultant co-location of LC3 and TP53INP2. Additionally, the autophagy-lysosome system was involved in the degradation process of TP53INP2 and applying autophagy inhibitors (Baf-A1) could significantly increase the TP53INP2 levels in murine and human BMSCs under oxidative stress. Third, gain- and loss-of-function assays revealed that knockdown of TP53INP2 inhibited osteogenic differentiation of BMSCs, while overexpression of TP53INP2 promoted osteogenic differentiation of BMSCs in vitro. Further analysis demonstrated that TP53INP2 promoted osteogenic differentiation of BMSCs by activating Wnt/β-cantenin signaling. DKK1, an inhibitor of Wnt signaling, resulted in osteogenic defects of BMSCs that had over-expressed TP53INP2. Lithium, a Wnt/β-catenin activator, improved the mineralization ability in TP53INP2-knockdown BMSCs. Moreover, restoring TP53INP2 levels recovered OVX-derived BMSCs osteogenic differentiation and attenuated bone loss in OVX mice. Taken together, our study indicated that oxidative stress-induced downregulation of TP53INP2 suppressed osteogenic differentiation of BMSCs during osteoporosis and was mediated by the autophagy degradation pathway. These findings may introduce a novel therapeutic target for osteoporosis." @default.
• W3132010090 created "2021-03-01" @default.
• W3132010090 creator A5002671318 @default.
• W3132010090 creator A5004780696 @default.
• W3132010090 creator A5043528627 @default.
• W3132010090 creator A5044692542 @default.
• W3132010090 creator A5051539538 @default.
• W3132010090 creator A5072131803 @default.
• W3132010090 date "2021-04-01" @default.
• W3132010090 modified "2023-10-06" @default.
• W3132010090 title "Oxidative stress induces downregulation of TP53INP2 and suppresses osteogenic differentiation of BMSCs during osteoporosis through the autophagy degradation pathway" @default.
• W3132010090 cites W1977612475 @default.
• W3132010090 cites W1986295603 @default.
• W3132010090 cites W2017326100 @default.
• W3132010090 cites W2032814621 @default.
• W3132010090 cites W2041425125 @default.
• W3132010090 cites W2041599197 @default.
• W3132010090 cites W2044147730 @default.
• W3132010090 cites W2074206215 @default.
• W3132010090 cites W2082244622 @default.
• W3132010090 cites W2082638468 @default.
• W3132010090 cites W2088657362 @default.
• W3132010090 cites W2093682445 @default.
• W3132010090 cites W2122351631 @default.
• W3132010090 cites W2152505621 @default.
• W3132010090 cites W2153101639 @default.
• W3132010090 cites W2165146798 @default.
• W3132010090 cites W2328967715 @default.
• W3132010090 cites W2344880307 @default.
• W3132010090 cites W2486828210 @default.
• W3132010090 cites W2593115970 @default.
• W3132010090 cites W2753568024 @default.
• W3132010090 cites W2791007378 @default.
• W3132010090 cites W2793486858 @default.
• W3132010090 cites W2795232695 @default.
• W3132010090 cites W2799973181 @default.
• W3132010090 cites W2810124170 @default.
• W3132010090 cites W2862921213 @default.
• W3132010090 cites W2897075453 @default.
• W3132010090 cites W2900347078 @default.
• W3132010090 cites W2909665615 @default.
• W3132010090 cites W2909785412 @default.
• W3132010090 cites W2912662699 @default.
• W3132010090 cites W2923464495 @default.
• W3132010090 cites W2937626558 @default.
• W3132010090 cites W2947422696 @default.
• W3132010090 cites W2947659393 @default.
• W3132010090 cites W2948055591 @default.
• W3132010090 cites W2950175770 @default.
• W3132010090 cites W2982954614 @default.
• W3132010090 cites W2988906435 @default.
• W3132010090 cites W2994914254 @default.
• W3132010090 cites W2997596905 @default.
• W3132010090 cites W2997710372 @default.
• W3132010090 cites W2998222045 @default.
• W3132010090 cites W2998874459 @default.
• W3132010090 cites W3000396828 @default.
• W3132010090 cites W3000861530 @default.
• W3132010090 cites W3012475378 @default.
• W3132010090 doi "https://doi.org/10.1016/j.freeradbiomed.2021.02.025" @default.
• W3132010090 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33636337" @default.
• W3132010090 hasPublicationYear "2021" @default.
• W3132010090 type Work @default.
• W3132010090 sameAs 3132010090 @default.
• W3132010090 citedByCount "24" @default.
• W3132010090 countsByYear W31320100902021 @default.
• W3132010090 countsByYear W31320100902022 @default.
• W3132010090 countsByYear W31320100902023 @default.
• W3132010090 crossrefType "journal-article" @default.
• W3132010090 hasAuthorship W3132010090A5002671318 @default.
• W3132010090 hasAuthorship W3132010090A5004780696 @default.
• W3132010090 hasAuthorship W3132010090A5043528627 @default.
• W3132010090 hasAuthorship W3132010090A5044692542 @default.
• W3132010090 hasAuthorship W3132010090A5051539538 @default.
• W3132010090 hasAuthorship W3132010090A5072131803 @default.
• W3132010090 hasConcept C104317684 @default.
• W3132010090 hasConcept C127561419 @default.
• W3132010090 hasConcept C137620995 @default.
• W3132010090 hasConcept C173396325 @default.
• W3132010090 hasConcept C185592680 @default.
• W3132010090 hasConcept C190283241 @default.
• W3132010090 hasConcept C203522944 @default.
• W3132010090 hasConcept C2776151105 @default.
• W3132010090 hasConcept C2777491412 @default.
• W3132010090 hasConcept C55493867 @default.
• W3132010090 hasConcept C62478195 @default.
• W3132010090 hasConcept C86803240 @default.
• W3132010090 hasConcept C95444343 @default.
• W3132010090 hasConceptScore W3132010090C104317684 @default.
• W3132010090 hasConceptScore W3132010090C127561419 @default.
• W3132010090 hasConceptScore W3132010090C137620995 @default.
• W3132010090 hasConceptScore W3132010090C173396325 @default.
• W3132010090 hasConceptScore W3132010090C185592680 @default.
• W3132010090 hasConceptScore W3132010090C190283241 @default.
• W3132010090 hasConceptScore W3132010090C203522944 @default.
• W3132010090 hasConceptScore W3132010090C2776151105 @default.
• W3132010090 hasConceptScore W3132010090C2777491412 @default.
• W3132010090 hasConceptScore W3132010090C55493867 @default.

• next