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- W3132052824 abstract "Microbial communities are found across diverse environments, including within and across the human body. As many microbes are unculturable in the lab, much of what is known about a microbiome-a collection of bacteria, fungi, archaea, and viruses inhabiting an environment--is from the sequencing of DNA from within the constituent community. Here, we provide an introduction to whole-metagenome shotgun sequencing studies, a ubiquitous approach for characterizing microbial communities, by reviewing three major research areas in metagenomics: assembly, community profiling, and functional profiling. Though not exhaustive, these areas encompass a large component of the metagenomics literature. We discuss each area in depth, the challenges posed by whole-metagenome shotgun sequencing, and approaches fundamental to the solutions of each. We conclude by discussing promising areas for future research. Though our emphasis is on the human microbiome, the methods discussed are broadly applicable across study systems." @default.
- W3132052824 created "2021-03-01" @default.
- W3132052824 creator A5000417492 @default.
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- W3132052824 date "2021-01-01" @default.
- W3132052824 modified "2023-09-27" @default.
- W3132052824 title "An Introduction to Whole-Metagenome Shotgun Sequencing Studies" @default.
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- W3132052824 doi "https://doi.org/10.1007/978-1-0716-1103-6_6" @default.
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