FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3132503670> ?p ?o ?g. }

• W3132503670 endingPage "17" @default.
• W3132503670 startingPage "17" @default.
• W3132503670 abstract "Wastewater-based epidemiology has become an important tool for the surveillance of SARS-CoV-2 outbreaks. However, the detection of viruses in sewage is challenging and to date there is no standard method available which has been validated for the sensitive detection of SARS-CoV-2. In this paper, we describe a simple concentration method based on polyethylene glycol (PEG) precipitation, followed by RNA extraction and a one-step quantitative reverse transcription PCR (qRT-PCR) for viral detection in wastewater. PEG-based concentration of viruses is a simple procedure which is not limited by the availability of expensive equipment and has reduced risk of disruption to consumable supply chains. The concentration and RNA extraction steps enable 900–1500× concentration of wastewater samples and sufficiently eliminates the majority of organic matter, which could inhibit the subsequent qRT-PCR assay. Due to the high variation in the physico-chemical properties of wastewater samples, we recommend the use of process control viruses to determine the efficiency of each step. This procedure enables the concentration and the extraction the DNA/RNA of different viruses and hence can be used for the surveillance of different viral targets for the comprehensive assessment of viral diseases in a community." @default.
• W3132503670 created "2021-03-01" @default.
• W3132503670 creator A5003187583 @default.
• W3132503670 creator A5011509548 @default.
• W3132503670 creator A5012154626 @default.
• W3132503670 creator A5019142373 @default.
• W3132503670 creator A5055882579 @default.
• W3132503670 creator A5056454066 @default.
• W3132503670 creator A5058007733 @default.
• W3132503670 creator A5064381910 @default.
• W3132503670 date "2021-02-23" @default.
• W3132503670 modified "2023-10-05" @default.
• W3132503670 title "Concentration and Quantification of SARS-CoV-2 RNA in Wastewater Using Polyethylene Glycol-Based Concentration and qRT-PCR" @default.
• W3132503670 cites W1968046880 @default.
• W3132503670 cites W2003549378 @default.
• W3132503670 cites W2072531693 @default.
• W3132503670 cites W2168420558 @default.
• W3132503670 cites W2345255358 @default.
• W3132503670 cites W2376794110 @default.
• W3132503670 cites W2582713488 @default.
• W3132503670 cites W2786271036 @default.
• W3132503670 cites W2801943051 @default.
• W3132503670 cites W2955185995 @default.
• W3132503670 cites W3005602227 @default.
• W3132503670 cites W3014221525 @default.
• W3132503670 cites W3017092394 @default.
• W3132503670 cites W3028152077 @default.
• W3132503670 cites W3033800749 @default.
• W3132503670 cites W3034926680 @default.
• W3132503670 cites W3044459694 @default.
• W3132503670 cites W3091520040 @default.
• W3132503670 doi "https://doi.org/10.3390/mps4010017" @default.
• W3132503670 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8005995" @default.
• W3132503670 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33672247" @default.
• W3132503670 hasPublicationYear "2021" @default.
• W3132503670 type Work @default.
• W3132503670 sameAs 3132503670 @default.
• W3132503670 citedByCount "36" @default.
• W3132503670 countsByYear W31325036702021 @default.
• W3132503670 countsByYear W31325036702022 @default.
• W3132503670 countsByYear W31325036702023 @default.
• W3132503670 crossrefType "journal-article" @default.
• W3132503670 hasAuthorship W3132503670A5003187583 @default.
• W3132503670 hasAuthorship W3132503670A5011509548 @default.
• W3132503670 hasAuthorship W3132503670A5012154626 @default.
• W3132503670 hasAuthorship W3132503670A5019142373 @default.
• W3132503670 hasAuthorship W3132503670A5055882579 @default.
• W3132503670 hasAuthorship W3132503670A5056454066 @default.
• W3132503670 hasAuthorship W3132503670A5058007733 @default.
• W3132503670 hasAuthorship W3132503670A5064381910 @default.
• W3132503670 hasBestOaLocation W31325036701 @default.
• W3132503670 hasConcept C10138342 @default.
• W3132503670 hasConcept C104317684 @default.
• W3132503670 hasConcept C105580179 @default.
• W3132503670 hasConcept C112190416 @default.
• W3132503670 hasConcept C159047783 @default.
• W3132503670 hasConcept C162324750 @default.
• W3132503670 hasConcept C185592680 @default.
• W3132503670 hasConcept C2776964284 @default.
• W3132503670 hasConcept C39432304 @default.
• W3132503670 hasConcept C43617362 @default.
• W3132503670 hasConcept C4692481 @default.
• W3132503670 hasConcept C4725764 @default.
• W3132503670 hasConcept C48023723 @default.
• W3132503670 hasConcept C49105822 @default.
• W3132503670 hasConcept C54400483 @default.
• W3132503670 hasConcept C55493867 @default.
• W3132503670 hasConcept C58790150 @default.
• W3132503670 hasConcept C67705224 @default.
• W3132503670 hasConcept C78063203 @default.
• W3132503670 hasConcept C86803240 @default.
• W3132503670 hasConcept C87717796 @default.
• W3132503670 hasConcept C94061648 @default.
• W3132503670 hasConceptScore W3132503670C10138342 @default.
• W3132503670 hasConceptScore W3132503670C104317684 @default.
• W3132503670 hasConceptScore W3132503670C105580179 @default.
• W3132503670 hasConceptScore W3132503670C112190416 @default.
• W3132503670 hasConceptScore W3132503670C159047783 @default.
• W3132503670 hasConceptScore W3132503670C162324750 @default.
• W3132503670 hasConceptScore W3132503670C185592680 @default.
• W3132503670 hasConceptScore W3132503670C2776964284 @default.
• W3132503670 hasConceptScore W3132503670C39432304 @default.
• W3132503670 hasConceptScore W3132503670C43617362 @default.
• W3132503670 hasConceptScore W3132503670C4692481 @default.
• W3132503670 hasConceptScore W3132503670C4725764 @default.
• W3132503670 hasConceptScore W3132503670C48023723 @default.
• W3132503670 hasConceptScore W3132503670C49105822 @default.
• W3132503670 hasConceptScore W3132503670C54400483 @default.
• W3132503670 hasConceptScore W3132503670C55493867 @default.
• W3132503670 hasConceptScore W3132503670C58790150 @default.
• W3132503670 hasConceptScore W3132503670C67705224 @default.
• W3132503670 hasConceptScore W3132503670C78063203 @default.
• W3132503670 hasConceptScore W3132503670C86803240 @default.
• W3132503670 hasConceptScore W3132503670C87717796 @default.
• W3132503670 hasConceptScore W3132503670C94061648 @default.
• W3132503670 hasFunder F4320334631 @default.
• W3132503670 hasIssue "1" @default.
• W3132503670 hasLocation W31325036701 @default.

• next