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• W3132783133 abstract "We reveal the cryo-electron microscopy structure of a type IV-B CRISPR ribonucleoprotein (RNP) complex (Csf) at 3.9-Å resolution. The complex best resembles the type III-A CRISPR Csm effector complex, consisting of a Cas7-like (Csf2) filament intertwined with a small subunit (Cas11) filament, but the complex lacks subunits for RNA processing and target DNA cleavage. Surprisingly, instead of assembling around a CRISPR-derived RNA (crRNA), the complex assembles upon heterogeneous RNA of a regular length arranged in a pseudo-A-form configuration. These findings provide a high-resolution glimpse into the assembly and function of enigmatic type IV CRISPR systems, expanding our understanding of class I CRISPR-Cas system architecture, and suggesting a function for type IV-B RNPs that may be distinct from other class 1 CRISPR-associated systems." @default.
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• W3132783133 date "2021-03-01" @default.
• W3132783133 modified "2023-09-28" @default.
• W3132783133 title "Structure of a type IV CRISPR-Cas ribonucleoprotein complex" @default.
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• W3132783133 doi "https://doi.org/10.1016/j.isci.2021.102201" @default.
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