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- W3132971330 endingPage "1031" @default.
- W3132971330 startingPage "1007" @default.
- W3132971330 abstract "Post-mortem metabolism is widely recognized to cause rapid and prolonged changes in the concentrations of multiple classes of compounds in brain, that is, they are labile. Post-mortem changes from levels in living brain include components of pathways of metabolism of glucose and energy compounds, amino acids, lipids, signaling molecules, neuropeptides, phosphoproteins, and proteins. Methods that stop enzyme activity at brain harvest were developed almost 50 years ago and have been extensively used in studies of brain functions and diseases. Unfortunately, these methods are not commonly used to harvest brain tissue for mass spectrometry-based metabolomic studies or for imaging mass spectrometry studies (IMS, also called mass spectrometry imaging, MSI, or matrix-assisted laser desorption/ionization-MSI, MALDI-MSI). Instead these studies commonly kill animals, decapitate, dissect out brain and regions of interest if needed, then 'snap' freeze the tissue to stop enzymatic activity after harvest, with post-mortem intervals typically ranging from ~0.5 to 3 min. To increase awareness of the importance of stopping metabolism at harvest and preventing the unnecessary complications of not doing so, this commentary provides examples of labile metabolites and the magnitudes of their post-mortem changes in concentrations during brain harvest. Brain harvest methods that stop metabolism at harvest eliminate post-mortem enzymatic activities and can improve characterization of normal and diseased brain. In addition, metabolomic studies would be improved by reporting absolute units of concentration along with normalized peak areas or fold changes. Then reported values can be evaluated and compared with the extensive neurochemical literature to help prevent reporting of artifactual data." @default.
- W3132971330 created "2021-03-01" @default.
- W3132971330 creator A5014975053 @default.
- W3132971330 date "2021-02-26" @default.
- W3132971330 modified "2023-10-14" @default.
- W3132971330 title "Stop the rot. Enzyme inactivation at brain harvest prevents artifacts" @default.
- W3132971330 cites W106740199 @default.
- W3132971330 cites W1200048591 @default.
- W3132971330 cites W150467427 @default.
- W3132971330 cites W1509297261 @default.
- W3132971330 cites W1536351550 @default.
- W3132971330 cites W1573203666 @default.
- W3132971330 cites W1595758937 @default.
- W3132971330 cites W1598010376 @default.
- W3132971330 cites W1672932894 @default.
- W3132971330 cites W1683050357 @default.
- W3132971330 cites W1749786054 @default.
- W3132971330 cites W1812391321 @default.
- W3132971330 cites W189309004 @default.
- W3132971330 cites W1897636699 @default.
- W3132971330 cites W1949006326 @default.
- W3132971330 cites W1964433896 @default.
- W3132971330 cites W1964977233 @default.
- W3132971330 cites W1965723205 @default.
- W3132971330 cites W1965981441 @default.
- W3132971330 cites W1966114495 @default.
- W3132971330 cites W1968079312 @default.
- W3132971330 cites W1969455434 @default.
- W3132971330 cites W1974483939 @default.
- W3132971330 cites W1975922633 @default.
- W3132971330 cites W1980602165 @default.
- W3132971330 cites W1982682496 @default.
- W3132971330 cites W1984795334 @default.
- W3132971330 cites W1988781035 @default.
- W3132971330 cites W1991379392 @default.
- W3132971330 cites W1991614616 @default.
- W3132971330 cites W1998563382 @default.
- W3132971330 cites W1998677303 @default.
- W3132971330 cites W1999268673 @default.
- W3132971330 cites W2001637853 @default.
- W3132971330 cites W2002542943 @default.
- W3132971330 cites W2003138125 @default.
- W3132971330 cites W2004282717 @default.
- W3132971330 cites W2006135012 @default.
- W3132971330 cites W2007519223 @default.
- W3132971330 cites W2011436251 @default.
- W3132971330 cites W2016853306 @default.
- W3132971330 cites W2022474884 @default.
- W3132971330 cites W2023826599 @default.
- W3132971330 cites W2024762883 @default.
- W3132971330 cites W2025158417 @default.
- W3132971330 cites W2028568974 @default.
- W3132971330 cites W2032442492 @default.
- W3132971330 cites W2037671654 @default.
- W3132971330 cites W2038760761 @default.
- W3132971330 cites W2038862011 @default.
- W3132971330 cites W2041410751 @default.
- W3132971330 cites W2042376469 @default.
- W3132971330 cites W2043263849 @default.
- W3132971330 cites W2044224589 @default.
- W3132971330 cites W2048708891 @default.
- W3132971330 cites W2049036645 @default.
- W3132971330 cites W2049653649 @default.
- W3132971330 cites W2049785746 @default.
- W3132971330 cites W2050946211 @default.
- W3132971330 cites W2051255403 @default.
- W3132971330 cites W2054672220 @default.
- W3132971330 cites W2056245716 @default.
- W3132971330 cites W2058555791 @default.
- W3132971330 cites W2063028213 @default.
- W3132971330 cites W2065029614 @default.
- W3132971330 cites W2068832470 @default.
- W3132971330 cites W2069405063 @default.
- W3132971330 cites W2069496768 @default.
- W3132971330 cites W2070013911 @default.
- W3132971330 cites W2072972179 @default.
- W3132971330 cites W2073537358 @default.
- W3132971330 cites W2073812888 @default.
- W3132971330 cites W2079608262 @default.
- W3132971330 cites W2080206746 @default.
- W3132971330 cites W2080391753 @default.
- W3132971330 cites W2087421641 @default.
- W3132971330 cites W2090091450 @default.
- W3132971330 cites W2094613162 @default.
- W3132971330 cites W2096945682 @default.
- W3132971330 cites W2113491045 @default.
- W3132971330 cites W2120032466 @default.
- W3132971330 cites W2122873971 @default.
- W3132971330 cites W2124170577 @default.
- W3132971330 cites W2126918774 @default.
- W3132971330 cites W2127093902 @default.
- W3132971330 cites W2133949190 @default.
- W3132971330 cites W2135206532 @default.
- W3132971330 cites W2139925812 @default.
- W3132971330 cites W2142957220 @default.
- W3132971330 cites W2146250938 @default.
- W3132971330 cites W2148512456 @default.
- W3132971330 cites W2151987154 @default.