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• W3133318785 abstract "Abstract The effects of different concentrations of copper sulfate (0.2 to 5 mg L −1 ) on in vitro callus and shoot formation of cucumber was investigated. Four-day-old cotyledon explants from the inbred line ‘Wisconsin 2843’ and the commercial cultivars ‘Marketer’ and ‘Negrito’ were used. The results on callus-derived shoots showed that the optimal concentration of CuSO 4 added to Murashige & Skoog (MS)-derived shoot induction medium containing 0.5 mg L −1 IAA and 2.5 mg L −1 BAP was 8-200 fold greater than in standard MS medium, and was genotype dependent. The highest genotypes response on shoot frequency and shoot number was achieved in this order by ‘Marketer’, ‘Negrito’ and ‘Wisconsin 2843’ with 1, 0.2 y 5 mg L −1 CuSO 4 , respectively. The genotype with the lowest control performance demanded the highest concentration of CuSO 4 for its optimal morphogenic response - 6- and 10-fold more in shoot frequency and shoot number, respectively. The other cultivars registered a 2-fold increase in both variables. All explants formed callus and the response on callus extension varied among cultivars. The regression analysis showed a statistically significant relationship between shoot number and concentrations of CuSO 4 and absence of association with callus extension. The present results indicate that application of specific concentrations of CuSO 4 higher than in standard MS medium, increases adventitious cucumber shoot organogenesis." @default.
• W3133318785 created "2021-03-01" @default.
• W3133318785 creator A5051478534 @default.
• W3133318785 date "2021-02-25" @default.
• W3133318785 modified "2023-10-15" @default.
• W3133318785 title "Influence of High Concentrations of Copper Sulfate on<i>In Vitro</i>Adventitious Organogenesis of<i>Cucumis sativus</i>L." @default.
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• W3133318785 doi "https://doi.org/10.1101/2021.02.24.432794" @default.
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