FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3133374080> ?p ?o ?g. }

• W3133374080 abstract "Purpose: Universal stress protein (USP) from Schistosoma mansoni, designated as G4LZI3, waspreviously hypothesised as a druggable target and vaccine candidate for human schistosomiasis.The purpose of this study is to characterize a purified recombinant G4LZI3 preliminarily forsubsequent structural characterization, which will provide baseline structural data for futurefunctional studies for the discovery, design and development of new schistosomal drugs for thetreatment, control and elimination of schistosomiasis. Methods: Restriction digest analysis of a GenScript-synthesised codon-optimised G4LZI3gene construct was carried out to ascertain its integrity and size. Thereafter, the pQE30-G4LZI3 construct was transformed into an M15 bacterial expression host. Transformed cellswere induced with isopropyl β-D-thiogalactoside for recombinant protein expression of anappreciable amount of pQE30-G4LZI3, which was subsequently purified with fast proteinliquid chromatography (FPLC) and a size exclusion chromatographic purification scheme.Preliminary biophysical characterization of the 6X His-tagged G4LZI3 was done to determineits secondary structure characteristics and protein stability. Results: A molecular weight protein of 20.3 kDa was confirmed subsequent to restriction digestanalysis, while heterologous protein expression yielded a highly soluble and considerableamount of histidine-tagged G4LZI3 protein, which was successfully purified to homogeneity.Biophysical characterization indicated that the protein was well folded, heat-stable, had thefunctional groups and secondary structure composition required and was thus amenable tofurther structural characterization and determination. Conclusion: Biophysical characterization of purified G4LZI3 showed that further structuralstudies can be embarked upon on the use of G4LZI3 as a druggable target and possibly avaccine target against schistosomiasis via vaccinomics." @default.
• W3133374080 created "2021-03-01" @default.
• W3133374080 creator A5022839251 @default.
• W3133374080 creator A5029887767 @default.
• W3133374080 creator A5072433554 @default.
• W3133374080 creator A5082399116 @default.
• W3133374080 creator A5085951889 @default.
• W3133374080 date "2021-01-31" @default.
• W3133374080 modified "2023-09-30" @default.
• W3133374080 title "Recombinant Expression and Biophysical Characterization of a Druggable Schistosoma mansoni Universal Stress G4LZI3 Protein" @default.
• W3133374080 cites W1491158878 @default.
• W3133374080 cites W1539868719 @default.
• W3133374080 cites W1972193434 @default.
• W3133374080 cites W1979338300 @default.
• W3133374080 cites W1981228319 @default.
• W3133374080 cites W1998459649 @default.
• W3133374080 cites W2013270561 @default.
• W3133374080 cites W2020025800 @default.
• W3133374080 cites W2023847634 @default.
• W3133374080 cites W2035938608 @default.
• W3133374080 cites W2043056371 @default.
• W3133374080 cites W2045698347 @default.
• W3133374080 cites W2049827200 @default.
• W3133374080 cites W2049909543 @default.
• W3133374080 cites W2054836358 @default.
• W3133374080 cites W2057596265 @default.
• W3133374080 cites W2059553149 @default.
• W3133374080 cites W2061057994 @default.
• W3133374080 cites W2061441963 @default.
• W3133374080 cites W2062460932 @default.
• W3133374080 cites W2065173635 @default.
• W3133374080 cites W2083515355 @default.
• W3133374080 cites W2086331830 @default.
• W3133374080 cites W2090610751 @default.
• W3133374080 cites W2107735396 @default.
• W3133374080 cites W2115668757 @default.
• W3133374080 cites W2123713974 @default.
• W3133374080 cites W2135676217 @default.
• W3133374080 cites W2146675496 @default.
• W3133374080 cites W2157745373 @default.
• W3133374080 cites W2158824937 @default.
• W3133374080 cites W2160564267 @default.
• W3133374080 cites W2171377206 @default.
• W3133374080 cites W2238033590 @default.
• W3133374080 cites W2305923160 @default.
• W3133374080 cites W2400181831 @default.
• W3133374080 cites W2520850140 @default.
• W3133374080 cites W2527370478 @default.
• W3133374080 cites W2535745851 @default.
• W3133374080 cites W2574755538 @default.
• W3133374080 cites W2768835663 @default.
• W3133374080 cites W2907958383 @default.
• W3133374080 doi "https://doi.org/10.34172/apb.2022.035" @default.
• W3133374080 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35620338" @default.
• W3133374080 hasPublicationYear "2021" @default.
• W3133374080 type Work @default.
• W3133374080 sameAs 3133374080 @default.
• W3133374080 citedByCount "1" @default.
• W3133374080 countsByYear W31333740802023 @default.
• W3133374080 crossrefType "journal-article" @default.
• W3133374080 hasAuthorship W3133374080A5022839251 @default.
• W3133374080 hasAuthorship W3133374080A5029887767 @default.
• W3133374080 hasAuthorship W3133374080A5072433554 @default.
• W3133374080 hasAuthorship W3133374080A5082399116 @default.
• W3133374080 hasAuthorship W3133374080A5085951889 @default.
• W3133374080 hasBestOaLocation W31333740801 @default.
• W3133374080 hasConcept C104317684 @default.
• W3133374080 hasConcept C10679952 @default.
• W3133374080 hasConcept C108305142 @default.
• W3133374080 hasConcept C123894998 @default.
• W3133374080 hasConcept C153911025 @default.
• W3133374080 hasConcept C165901193 @default.
• W3133374080 hasConcept C185592680 @default.
• W3133374080 hasConcept C203014093 @default.
• W3133374080 hasConcept C2776225656 @default.
• W3133374080 hasConcept C2776863245 @default.
• W3133374080 hasConcept C40767141 @default.
• W3133374080 hasConcept C55493867 @default.
• W3133374080 hasConcept C70721500 @default.
• W3133374080 hasConcept C86803240 @default.
• W3133374080 hasConcept C8985478 @default.
• W3133374080 hasConceptScore W3133374080C104317684 @default.
• W3133374080 hasConceptScore W3133374080C10679952 @default.
• W3133374080 hasConceptScore W3133374080C108305142 @default.
• W3133374080 hasConceptScore W3133374080C123894998 @default.
• W3133374080 hasConceptScore W3133374080C153911025 @default.
• W3133374080 hasConceptScore W3133374080C165901193 @default.
• W3133374080 hasConceptScore W3133374080C185592680 @default.
• W3133374080 hasConceptScore W3133374080C203014093 @default.
• W3133374080 hasConceptScore W3133374080C2776225656 @default.
• W3133374080 hasConceptScore W3133374080C2776863245 @default.
• W3133374080 hasConceptScore W3133374080C40767141 @default.
• W3133374080 hasConceptScore W3133374080C55493867 @default.
• W3133374080 hasConceptScore W3133374080C70721500 @default.
• W3133374080 hasConceptScore W3133374080C86803240 @default.
• W3133374080 hasConceptScore W3133374080C8985478 @default.
• W3133374080 hasLocation W31333740801 @default.
• W3133374080 hasLocation W31333740802 @default.
• W3133374080 hasLocation W31333740803 @default.
• W3133374080 hasOpenAccess W3133374080 @default.

• next