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• W3133483868 abstract "In this study, it was aimed to evaluate the expressions of 8-OHdG and MDA immunohistochemically in order to determine the oxidative stress-induced DNA damage and lipid peroxidation in 16 papillomas and 14 fibropapillomas from 30 cattle brought to our department between 2013-2020. Biopsy samples taken after surgery were fixed in buffered 10% formaldehyde solution. Sections of 5 μm thickness were taken from the paraffin blocks prepared after routine tissue follow-up procedures and Hematoxylin &amp; Eosin staining was applied to the sections in order to detect histopathological changes. Avidin Biotin Peroxidase method was used for immunohistochemical staining. In papilloma cases, severe hyperkeratosis, retepects extending from the epidermis to the dermis, spongiosis and balloon-like degeneration in squamous epithelial cells, basophilic inclusion bodies in granular cells, ulcerations in the epidermis layer, hemorrhagic areas and an increase in kerato hyaline granules were observed. In addition to these findings, dense connective tissue increases were detected in fibropapilloma cases. In immunohistochemical evaluations, positive reactions for BPV were observed in the nuclei of cells in stratum granulosum. 8-OHdG positive reactions were detected in the cytoplasm and nucleus of the epidermal cells in papilloma cases, whereas in cases of fibropapilloma, reactions in these cells were observed in the cytoplasm of fibrocytes and fibroblasts in the dermis. Membranous MDA positive reactions were observed in the epidermal cells in papilloma cases, whereas MDA expressions were detected in the cytoplasm of fibrocytes and fibroblasts in the dermis of fibropapilloma cases. As a result of the literature reviews, no study data was found in which DNA damage due to oxidative stres and lipid peroxidation was detected by means of 8-OHdG and MDA expressions in papilloma and fibropapilloma cases of cattle, it is thought that the findings obtained in this study will contribute to the literature. In addition, it has been concluded that oxidative stress plays an important role in the pathogenesis of this tumor." @default.
• W3133483868 created "2021-03-01" @default.
• W3133483868 creator A5053605256 @default.
• W3133483868 date "2021-03-25" @default.
• W3133483868 modified "2023-09-28" @default.
• W3133483868 title "Sığır Papillom ve Fibropapillomlarda Oksidatif Stres Kaynaklı DNA Hasarı ve Lipid Peroksidasyonun İmmunohistokimyasal Olarak Araştırılması" @default.
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• W3133483868 doi "https://doi.org/10.36483/vanvetj.843747" @default.
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