Matches in SemOpenAlex for { <https://semopenalex.org/work/W3133691334> ?p ?o ?g. }
- W3133691334 endingPage "2446" @default.
- W3133691334 startingPage "2435" @default.
- W3133691334 abstract "Although cryopreservation is widely used in animal breeding, the technique is still suboptimal. The population of spermatozoa surviving the procedure experiences changes attributed to alteration in their redox regulation. In order to expand our knowledge regarding this particular aspect, the proteome in fresh and frozen thawed aliquots of equine spermatozoa was studied to identify the proteins most severely affected by the procedure. If alteration of redox regulation is a major factor explaining cryodamage, proteins participating in redox regulation should be principally affected. Using a split sample design, 30 ejaculates from 10 different stallions were analyzed as fresh spermatozoa, and another aliquot from the same ejaculate was analyzed as a frozen thawed sample. The proteome was studied under both conditions using UHPLC-MS/MS and bioinformatic analysis conducted to identify discriminant variables between both conditions. Data are available through the ProteomeXchange Consortium with identifier PXD022236. The proteins most significantly reduced were Aldo-keto reductase family 1 member B (p = 2.2 × 10-17) and Superoxide dismutase (Cu-Zn) (p = 4.7 × 10-14). This is the first time that SOD1 has been identified as a discriminating variable using bioinformatic analysis, where it was one of the most highly significantly different proteins seen between fresh and frozen thawed semen. This finding strongly supports the theory that alteration in redox regulation and oxidative stress is a major factor involved in cryodamage and suggests that control of redox regulation should be a major target to improve current cryopreservation procedures." @default.
- W3133691334 created "2021-03-15" @default.
- W3133691334 creator A5009932635 @default.
- W3133691334 creator A5024445965 @default.
- W3133691334 creator A5027761244 @default.
- W3133691334 creator A5028592188 @default.
- W3133691334 creator A5033841655 @default.
- W3133691334 creator A5050817135 @default.
- W3133691334 creator A5091144227 @default.
- W3133691334 date "2021-03-03" @default.
- W3133691334 modified "2023-10-18" @default.
- W3133691334 title "In Stallion Spermatozoa, Superoxide Dismutase (Cu–Zn) (SOD1) and the Aldo-Keto-Reductase Family 1 Member b (AKR1B1) Are the Proteins Most Significantly Reduced by Cryopreservation" @default.
- W3133691334 cites W1965825083 @default.
- W3133691334 cites W1969778539 @default.
- W3133691334 cites W1975840953 @default.
- W3133691334 cites W1976820001 @default.
- W3133691334 cites W1981740138 @default.
- W3133691334 cites W1984121660 @default.
- W3133691334 cites W2000264054 @default.
- W3133691334 cites W2021119961 @default.
- W3133691334 cites W2034728682 @default.
- W3133691334 cites W2038562206 @default.
- W3133691334 cites W2058358568 @default.
- W3133691334 cites W2069573786 @default.
- W3133691334 cites W2082711578 @default.
- W3133691334 cites W2091395879 @default.
- W3133691334 cites W2093569887 @default.
- W3133691334 cites W2096214321 @default.
- W3133691334 cites W2111527733 @default.
- W3133691334 cites W2127248737 @default.
- W3133691334 cites W2136626461 @default.
- W3133691334 cites W2142068691 @default.
- W3133691334 cites W2144673676 @default.
- W3133691334 cites W2150926065 @default.
- W3133691334 cites W2152662614 @default.
- W3133691334 cites W2164129966 @default.
- W3133691334 cites W2247608613 @default.
- W3133691334 cites W2253329180 @default.
- W3133691334 cites W2258645267 @default.
- W3133691334 cites W2318552449 @default.
- W3133691334 cites W2394841973 @default.
- W3133691334 cites W2467615177 @default.
- W3133691334 cites W2551816155 @default.
- W3133691334 cites W2558339924 @default.
- W3133691334 cites W2587379595 @default.
- W3133691334 cites W2747271941 @default.
- W3133691334 cites W2762802201 @default.
- W3133691334 cites W2794274432 @default.
- W3133691334 cites W2795880214 @default.
- W3133691334 cites W2810430781 @default.
- W3133691334 cites W2899229843 @default.
- W3133691334 cites W2899760200 @default.
- W3133691334 cites W2905503279 @default.
- W3133691334 cites W2911433412 @default.
- W3133691334 cites W2936955921 @default.
- W3133691334 cites W2939234968 @default.
- W3133691334 cites W2944545583 @default.
- W3133691334 cites W2947757970 @default.
- W3133691334 cites W2974481737 @default.
- W3133691334 cites W2979252188 @default.
- W3133691334 cites W2994954488 @default.
- W3133691334 cites W2995851010 @default.
- W3133691334 cites W2998057151 @default.
- W3133691334 cites W3003381482 @default.
- W3133691334 cites W3014619688 @default.
- W3133691334 cites W3016968385 @default.
- W3133691334 cites W3035120803 @default.
- W3133691334 cites W3038677283 @default.
- W3133691334 doi "https://doi.org/10.1021/acs.jproteome.0c00932" @default.
- W3133691334 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8562871" @default.
- W3133691334 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33656888" @default.
- W3133691334 hasPublicationYear "2021" @default.
- W3133691334 type Work @default.
- W3133691334 sameAs 3133691334 @default.
- W3133691334 citedByCount "16" @default.
- W3133691334 countsByYear W31336913342021 @default.
- W3133691334 countsByYear W31336913342022 @default.
- W3133691334 countsByYear W31336913342023 @default.
- W3133691334 crossrefType "journal-article" @default.
- W3133691334 hasAuthorship W3133691334A5009932635 @default.
- W3133691334 hasAuthorship W3133691334A5024445965 @default.
- W3133691334 hasAuthorship W3133691334A5027761244 @default.
- W3133691334 hasAuthorship W3133691334A5028592188 @default.
- W3133691334 hasAuthorship W3133691334A5033841655 @default.
- W3133691334 hasAuthorship W3133691334A5050817135 @default.
- W3133691334 hasAuthorship W3133691334A5091144227 @default.
- W3133691334 hasBestOaLocation W31336913341 @default.
- W3133691334 hasConcept C104397665 @default.
- W3133691334 hasConcept C16685009 @default.
- W3133691334 hasConcept C179933525 @default.
- W3133691334 hasConcept C185592680 @default.
- W3133691334 hasConcept C196843134 @default.
- W3133691334 hasConcept C2775838275 @default.
- W3133691334 hasConcept C2776151105 @default.
- W3133691334 hasConcept C2777615887 @default.
- W3133691334 hasConcept C2778093475 @default.
- W3133691334 hasConcept C2908647359 @default.
- W3133691334 hasConcept C55493867 @default.