FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W31337204> ?p ?o ?g. }

• W31337204 endingPage "122" @default.
• W31337204 startingPage "109" @default.
• W31337204 abstract "Sperm DNA fragmentation (sDF) represents a threat to male fertility, human reproduction and the health of the offspring. The causes of sDF are still unclear, even if apoptosis, oxidative assault and defects in chromatin maturation are hypothesized. Using multicolor flow cytometry and sperm sorting, we challenged the three hypothesized mechanisms by simultaneously evaluating sDF and signs of oxidative damage (8-hydroxy, 2′-deoxyguanosine (8-OHdG) and malondialdehyde (MDA)), apoptosis (caspase activity and cleaved poly(ADP-ribose) polymerase (cPARP)) and sperm immaturity (creatine phosphokinase (CK) and excess of residual histones). Active caspases and c-PARP were concomitant with sDF in a high percentage of spermatozoa (82.6% ± 9.1% and 53.5% ± 16.4%, respectively). Excess of residual histones was significantly higher in DNA-fragmented sperm versus sperm without DNA fragmentation (74.8% ± 17.5% and 37.3% ± 16.6%, respectively, p < 0.005), and largely concomitant with active caspases. Conversely, oxidative damage was scarcely concomitant with sDF in the total sperm population, at variance with live sperm, where 8-OHdG and MDA were clearly associated to sDF. In addition, most live cells with active caspase also showed 8-OHdG, suggesting activation of apoptotic pathways in oxidative-injured live cells. This is the first investigation on the origin of sDF directly evaluating the simultaneous presence of the signs of the hypothesized mechanisms with DNA breaks at the single cell level. The results indicate that the main pathway leading to sperm DNA breaks is a process of apoptosis, likely triggered by an impairment of chromatin maturation in the testis and by oxidative stress during the transit in the male genital tract. These findings are highly relevant for clinical studies on the effects of drugs on sDF and oxidative stress in infertile men and for the development of new therapeutic strategies." @default.
• W31337204 created "2016-06-24" @default.
• W31337204 creator A5021273927 @default.
• W31337204 creator A5023560747 @default.
• W31337204 creator A5036742303 @default.
• W31337204 creator A5047755964 @default.
• W31337204 creator A5049302897 @default.
• W31337204 creator A5072038760 @default.
• W31337204 creator A5081731109 @default.
• W31337204 creator A5091522556 @default.
• W31337204 date "2015-01-01" @default.
• W31337204 modified "2023-10-13" @default.
• W31337204 title "Investigation on the Origin of Sperm DNA Fragmentation: Role of Apoptosis, Immaturity and Oxidative Stress" @default.
• W31337204 cites W1487948491 @default.
• W31337204 cites W1489071015 @default.
• W31337204 cites W1949465845 @default.
• W31337204 cites W1966439447 @default.
• W31337204 cites W1966704219 @default.
• W31337204 cites W1966938364 @default.
• W31337204 cites W1968804373 @default.
• W31337204 cites W1982459378 @default.
• W31337204 cites W1983884639 @default.
• W31337204 cites W1983976201 @default.
• W31337204 cites W1985712542 @default.
• W31337204 cites W1990289519 @default.
• W31337204 cites W1995610565 @default.
• W31337204 cites W2000549419 @default.
• W31337204 cites W2018168728 @default.
• W31337204 cites W2023957744 @default.
• W31337204 cites W2025303374 @default.
• W31337204 cites W2027328852 @default.
• W31337204 cites W2027333658 @default.
• W31337204 cites W2035824032 @default.
• W31337204 cites W2042379310 @default.
• W31337204 cites W2043786747 @default.
• W31337204 cites W2047270772 @default.
• W31337204 cites W2054446926 @default.
• W31337204 cites W2061974398 @default.
• W31337204 cites W2066780482 @default.
• W31337204 cites W2069777216 @default.
• W31337204 cites W2071461845 @default.
• W31337204 cites W2077933167 @default.
• W31337204 cites W2078815410 @default.
• W31337204 cites W2086287988 @default.
• W31337204 cites W2087573544 @default.
• W31337204 cites W2090172678 @default.
• W31337204 cites W2091261162 @default.
• W31337204 cites W2095904663 @default.
• W31337204 cites W2096191845 @default.
• W31337204 cites W2096206245 @default.
• W31337204 cites W2097223674 @default.
• W31337204 cites W2100401399 @default.
• W31337204 cites W2103358215 @default.
• W31337204 cites W2103755474 @default.
• W31337204 cites W2106855468 @default.
• W31337204 cites W2107846341 @default.
• W31337204 cites W2115524920 @default.
• W31337204 cites W2126608167 @default.
• W31337204 cites W2126917185 @default.
• W31337204 cites W2128478608 @default.
• W31337204 cites W2128754410 @default.
• W31337204 cites W2131850230 @default.
• W31337204 cites W2132809301 @default.
• W31337204 cites W2134224716 @default.
• W31337204 cites W2140367523 @default.
• W31337204 cites W2142383155 @default.
• W31337204 cites W2142451890 @default.
• W31337204 cites W2143468659 @default.
• W31337204 cites W2146893907 @default.
• W31337204 cites W2151034874 @default.
• W31337204 cites W2151372165 @default.
• W31337204 cites W2158173525 @default.
• W31337204 cites W2159000683 @default.
• W31337204 cites W2159944012 @default.
• W31337204 cites W2164007446 @default.
• W31337204 cites W2166893883 @default.
• W31337204 cites W2167375579 @default.
• W31337204 cites W2167832788 @default.
• W31337204 cites W2171321028 @default.
• W31337204 cites W2171820243 @default.
• W31337204 cites W2231999257 @default.
• W31337204 cites W2344853850 @default.
• W31337204 cites W4241866481 @default.
• W31337204 doi "https://doi.org/10.2119/molmed.2014.00158" @default.
• W31337204 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4461587" @default.
• W31337204 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25786204" @default.
• W31337204 hasPublicationYear "2015" @default.
• W31337204 type Work @default.
• W31337204 sameAs 31337204 @default.
• W31337204 citedByCount "181" @default.
• W31337204 countsByYear W313372042015 @default.
• W31337204 countsByYear W313372042016 @default.
• W31337204 countsByYear W313372042017 @default.
• W31337204 countsByYear W313372042018 @default.
• W31337204 countsByYear W313372042019 @default.
• W31337204 countsByYear W313372042020 @default.
• W31337204 countsByYear W313372042021 @default.
• W31337204 countsByYear W313372042022 @default.

• next