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• W3133733166 abstract "In this study, hollow mesoporous silica cube (HMSC) modified with amino (-NH2) were synthesized and applied in the immobilization of phospholipase D (PLD) via physical adsorption and chemical cross-linking strategy. The amino-functionalized nano carrier HMSC represented excellent immobilization ability and achieved 87.15 % immobilization rate. The immobilized PLD has wider pH application range and thermal stability, and maintained over 90% of the initial activity after incubation at 50 °C for 2 h. After 50 days of storage at 4 ℃, immobilized PLD retained 40.12 % of its initial activity while free PLD lost 88.28% of its initial activity. The modified HMSC with immobilized PLD (HMSC-NH2-PLD) retained 50.73% activities after 9 consecutive reuses. Using the HMSC-NH2-PLD, a high-efficient method for the conversion of phosphatidylserine (PS) from phosphatidylcholine (PC) and L-serine was proposed. The HMSC-NH2-PLD exhibited prominent enzymatic activity for PS synthesis, the maximal conversion of PS was 90.40% with a catalytic efficiency (CE) of 31.95 μmol / (g h under the optimal conditions. The research in this paper provides a sustainable and efficient biocatalysis application for PS synthesis." @default.
• W3133733166 created "2021-03-15" @default.
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• W3133733166 date "2021-06-01" @default.
• W3133733166 modified "2023-10-16" @default.
• W3133733166 title "Design of amino-functionalized hollow mesoporous silica cube for enzyme immobilization and its application in synthesis of phosphatidylserine" @default.
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• W3133733166 doi "https://doi.org/10.1016/j.colsurfb.2021.111668" @default.
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