FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3133768535> ?p ?o ?g. }

• W3133768535 abstract "UV-cured epoxy-based polymeric film was prepared from glycidyl methacrylate, trimethylolpropane triacrylate, and poly(ethylene glycol) methylether acrylate. 2-hydroxy-2- methylpropiophenone was used as photo initiator. Covalent binding through epoxy groups was employed to immobilize β-galactosidase from Escherichia coli onto this film, and immobilization conditions were optimized by the response surface methodology. ATR-Fourier transform infrared (FTIR) and scanning electron microscopy (SEM) analysis was carried out to characterize the epoxy-based polymeric film. Immobilization yield of β-galactosidase on the material was calculated as 3.57 mg/g and the highest enzyme activity for the immobilized enzyme recorded at pH 6.5°C and 60°C. The immobilized enzyme preserved 51% of its activity at the end of 12 runs. Free and immobilized enzyme hydrolyzed 163.8 and 172.3 µM lactose from 1% lactose, respectively. Kinetic parameters of both free and immobilized β-galactosidase were also investigated, and Km values were determined to be 0.647 and 0.7263 mM, respectively. Practical applications In our study we prepared a UV-cured epoxy-based polymeric film and optimized the immobilization conditions of β-galactosidase from Escherichia coli onto this polymeric film by using response surface methodology (RSM). For this purpose, three-level and three-factor Box–Behnken design, which is an independent, rotatable or nearly rotatable, quadratic design, was applied. Optimal levels of three variables, namely, the amount of enzyme, immobilization time, and pH were determined using Box–Behnken experimental design. Lactose hydrolysis studies were performed from milk and lactose samples using free and immobilized enzyme. In addition, kinetic parameters, storage stability, and re-usability of immobilized β-galactosidase were examined." @default.
• W3133768535 created "2021-03-15" @default.
• W3133768535 creator A5022956134 @default.
• W3133768535 creator A5026788482 @default.
• W3133768535 creator A5035730849 @default.
• W3133768535 creator A5038327671 @default.
• W3133768535 creator A5083157461 @default.
• W3133768535 creator A5084201055 @default.
• W3133768535 date "2021-03-10" @default.
• W3133768535 modified "2023-10-15" @default.
• W3133768535 title "Optimizing the immobilization conditions of β‐galactosidase on UV‐cured epoxy‐based polymeric film using response surface methodology" @default.
• W3133768535 cites W1970629160 @default.
• W3133768535 cites W1973446731 @default.
• W3133768535 cites W1980040581 @default.
• W3133768535 cites W1988449170 @default.
• W3133768535 cites W2001290593 @default.
• W3133768535 cites W2002753176 @default.
• W3133768535 cites W2003189828 @default.
• W3133768535 cites W2012736464 @default.
• W3133768535 cites W2018785523 @default.
• W3133768535 cites W2022706949 @default.
• W3133768535 cites W2029031614 @default.
• W3133768535 cites W2033678773 @default.
• W3133768535 cites W2043451183 @default.
• W3133768535 cites W2054519191 @default.
• W3133768535 cites W2056264623 @default.
• W3133768535 cites W2062990609 @default.
• W3133768535 cites W2067965831 @default.
• W3133768535 cites W2071690028 @default.
• W3133768535 cites W2078496681 @default.
• W3133768535 cites W2079219568 @default.
• W3133768535 cites W2081211357 @default.
• W3133768535 cites W2092825009 @default.
• W3133768535 cites W2115381210 @default.
• W3133768535 cites W2116062064 @default.
• W3133768535 cites W2278261308 @default.
• W3133768535 cites W2569783264 @default.
• W3133768535 cites W2773811333 @default.
• W3133768535 cites W2783372730 @default.
• W3133768535 cites W2783489898 @default.
• W3133768535 cites W2802573692 @default.
• W3133768535 cites W2910365921 @default.
• W3133768535 cites W2921131254 @default.
• W3133768535 cites W2979668293 @default.
• W3133768535 cites W2996492553 @default.
• W3133768535 cites W4293247451 @default.
• W3133768535 doi "https://doi.org/10.1111/jfbc.13699" @default.
• W3133768535 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33694174" @default.
• W3133768535 hasPublicationYear "2021" @default.
• W3133768535 type Work @default.
• W3133768535 sameAs 3133768535 @default.
• W3133768535 citedByCount "4" @default.
• W3133768535 countsByYear W31337685352023 @default.
• W3133768535 crossrefType "journal-article" @default.
• W3133768535 hasAuthorship W3133768535A5022956134 @default.
• W3133768535 hasAuthorship W3133768535A5026788482 @default.
• W3133768535 hasAuthorship W3133768535A5035730849 @default.
• W3133768535 hasAuthorship W3133768535A5038327671 @default.
• W3133768535 hasAuthorship W3133768535A5083157461 @default.
• W3133768535 hasAuthorship W3133768535A5084201055 @default.
• W3133768535 hasBestOaLocation W31337685351 @default.
• W3133768535 hasConcept C127413603 @default.
• W3133768535 hasConcept C132050475 @default.
• W3133768535 hasConcept C13965031 @default.
• W3133768535 hasConcept C150077022 @default.
• W3133768535 hasConcept C15920480 @default.
• W3133768535 hasConcept C159985019 @default.
• W3133768535 hasConcept C160892712 @default.
• W3133768535 hasConcept C166595027 @default.
• W3133768535 hasConcept C170295934 @default.
• W3133768535 hasConcept C178790620 @default.
• W3133768535 hasConcept C181199279 @default.
• W3133768535 hasConcept C185592680 @default.
• W3133768535 hasConcept C188027245 @default.
• W3133768535 hasConcept C192562407 @default.
• W3133768535 hasConcept C2777516009 @default.
• W3133768535 hasConcept C2778724459 @default.
• W3133768535 hasConcept C2779989194 @default.
• W3133768535 hasConcept C2780801859 @default.
• W3133768535 hasConcept C33633552 @default.
• W3133768535 hasConcept C42360764 @default.
• W3133768535 hasConcept C43617362 @default.
• W3133768535 hasConcept C521977710 @default.
• W3133768535 hasConcept C94412978 @default.
• W3133768535 hasConceptScore W3133768535C127413603 @default.
• W3133768535 hasConceptScore W3133768535C132050475 @default.
• W3133768535 hasConceptScore W3133768535C13965031 @default.
• W3133768535 hasConceptScore W3133768535C150077022 @default.
• W3133768535 hasConceptScore W3133768535C15920480 @default.
• W3133768535 hasConceptScore W3133768535C159985019 @default.
• W3133768535 hasConceptScore W3133768535C160892712 @default.
• W3133768535 hasConceptScore W3133768535C166595027 @default.
• W3133768535 hasConceptScore W3133768535C170295934 @default.
• W3133768535 hasConceptScore W3133768535C178790620 @default.
• W3133768535 hasConceptScore W3133768535C181199279 @default.
• W3133768535 hasConceptScore W3133768535C185592680 @default.
• W3133768535 hasConceptScore W3133768535C188027245 @default.
• W3133768535 hasConceptScore W3133768535C192562407 @default.
• W3133768535 hasConceptScore W3133768535C2777516009 @default.
• W3133768535 hasConceptScore W3133768535C2778724459 @default.

• next