FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3133787102> ?p ?o ?g. }

• W3133787102 abstract "Diatoms, eukaryotic algae with silica cell wall have been proved to be reliable bioindicators and are applied in ecological status assessment of aquatic ecosystems using indices calculated from pollution sensitivity and indicator values of species. Traditional method of identification of diatom species is based on morphological features of frustule that requires in-depth taxonomical knowledge and expertise. Metabarcoding that combines barcodes and high-throughput sequencing offers a promising alternative. In this pilot study we tested the applicability of metabarcoding of benthic diatom assemblages for ecological status assessment of water bodies in Hungary comparing its performance to that of morphology-based identification of species. Samples from 78 rivers and streams arranged along a trophic gradient and 14 soda pans representing unique types of habitats were investigated. Sequences of the barcode region of rbc L gene suggested by Vasselon et al. (2017) were acquired revealing 1135 diatom amplicon sequence variants (ASVs) of which 54% could be assigned at species level using Diat.barcode (Rimet et al. 2019) as reference database. Morphological investigation detected more species and intraspecific taxa in the lotic and lentic samples (413 and 78, respectively) than metabarcoding (190 and 75). Correspondence between taxa detected based on morphology and DNA sequences was relatively low (on average 24% in lotic and 26% in lentic samples) but considerably increased (on average 66% and 56%, respectively) when taking into account only the taxa reaching higher than 5% relative abundance in microscopy counting. The differences mainly derived from that a considerable portion (59% and 32%, respectively) of the morphologically identified taxa were not recorded in the reference database. Community structure of samples from both running and standing waters based on microscopy and DNA sequence analysis showed significant correlation as revealed using Mantel test. For rivers and streams diatom index IPS (Specific Pollution Sensitivity Index, (Coste in Cemagref, 1982) for soda pans indices H (Ziemann et al., 2001) and IBD (Indice Biologique Diatomées, Lenoir and Coste, 1996) were calculated from the results of the two aspects. Strong correlation was found between morphology- and sequence-based indices and in about half of the samples, the ecological status class obtained with the two methods coincided. Our results suggest that metabarcoding inheres a great opportunity and could be successfully applied in benthic diatom-based ecological status assessment of Hungarian waters after the reference database is supplemented with taxa frequently occurring in these habitats. References Cemagref, 1982. Etude des méthodes biologiques quantitativesd’appréciation de la qualité des eaux, Rapport Division Qualité des Eaux Lyon. Agence financière de Bassin Rhône-Méditerranée-Corse, Pierre-Bénite, France. Lenoir, A., Coste, M., 1996. Development of a practical diatom index of overall water quality applicable to the French National Water Board Network. Presented at the International symposium, Volksbildungsheim Grilhof Vill, AUT, 17-19 September 1995, Universität Innsbruck, pp. 29–43. Rimet, F., Gusev, E., Kahlert, M., Kelly, M.G., Kulikovskiy, M., Maltsev, Y., Mann, D.G., Pfannkuchen, M., Trobajo, R., Vasselon, V., Zimmermann, J., Bouchez, A., 2019. Diat.barcode, an open-access curated barcode library for diatoms. Sci. Rep. 9, 15116. Vasselon, V., Rimet, F., Tapolczai, K., Bouchez, A., 2017. Assessing ecological status with diatoms DNA metabarcoding: Scaling-up on a WFD monitoring network (Mayotte island, France). Ecol. Indic. 82, 1–12. Ziemann, H., Kies, L., Schulz, C.-J., 2001. Desalinization of running waters: III. Changes in the structure of diatom assemblages caused by a decreasing salt load and changing ion spectra in the river Wipper (Thuringia, Germany). Limnologica 31, 257–280." @default.
• W3133787102 created "2021-03-15" @default.
• W3133787102 creator A5025898595 @default.
• W3133787102 creator A5036424452 @default.
• W3133787102 creator A5041190853 @default.
• W3133787102 creator A5047715451 @default.
• W3133787102 creator A5049221695 @default.
• W3133787102 creator A5070271872 @default.
• W3133787102 creator A5070876734 @default.
• W3133787102 creator A5072043696 @default.
• W3133787102 creator A5075291708 @default.
• W3133787102 creator A5089547557 @default.
• W3133787102 creator A5090583529 @default.
• W3133787102 date "2021-03-04" @default.
• W3133787102 modified "2023-09-26" @default.
• W3133787102 title "Applicability of diatom metabarcoding in the ecological status assessment of Hungarian lotic and soda pan habitats" @default.
• W3133787102 doi "https://doi.org/10.3897/aca.4.e64887" @default.
• W3133787102 hasPublicationYear "2021" @default.
• W3133787102 type Work @default.
• W3133787102 sameAs 3133787102 @default.
• W3133787102 citedByCount "0" @default.
• W3133787102 crossrefType "journal-article" @default.
• W3133787102 hasAuthorship W3133787102A5025898595 @default.
• W3133787102 hasAuthorship W3133787102A5036424452 @default.
• W3133787102 hasAuthorship W3133787102A5041190853 @default.
• W3133787102 hasAuthorship W3133787102A5047715451 @default.
• W3133787102 hasAuthorship W3133787102A5049221695 @default.
• W3133787102 hasAuthorship W3133787102A5070271872 @default.
• W3133787102 hasAuthorship W3133787102A5070876734 @default.
• W3133787102 hasAuthorship W3133787102A5072043696 @default.
• W3133787102 hasAuthorship W3133787102A5075291708 @default.
• W3133787102 hasAuthorship W3133787102A5089547557 @default.
• W3133787102 hasAuthorship W3133787102A5090583529 @default.
• W3133787102 hasBestOaLocation W31337871021 @default.
• W3133787102 hasConcept C110872660 @default.
• W3133787102 hasConcept C129934472 @default.
• W3133787102 hasConcept C141732470 @default.
• W3133787102 hasConcept C175327387 @default.
• W3133787102 hasConcept C176585087 @default.
• W3133787102 hasConcept C185933670 @default.
• W3133787102 hasConcept C18903297 @default.
• W3133787102 hasConcept C2778902744 @default.
• W3133787102 hasConcept C42088612 @default.
• W3133787102 hasConcept C46685645 @default.
• W3133787102 hasConcept C71640776 @default.
• W3133787102 hasConcept C72958200 @default.
• W3133787102 hasConcept C86803240 @default.
• W3133787102 hasConceptScore W3133787102C110872660 @default.
• W3133787102 hasConceptScore W3133787102C129934472 @default.
• W3133787102 hasConceptScore W3133787102C141732470 @default.
• W3133787102 hasConceptScore W3133787102C175327387 @default.
• W3133787102 hasConceptScore W3133787102C176585087 @default.
• W3133787102 hasConceptScore W3133787102C185933670 @default.
• W3133787102 hasConceptScore W3133787102C18903297 @default.
• W3133787102 hasConceptScore W3133787102C2778902744 @default.
• W3133787102 hasConceptScore W3133787102C42088612 @default.
• W3133787102 hasConceptScore W3133787102C46685645 @default.
• W3133787102 hasConceptScore W3133787102C71640776 @default.
• W3133787102 hasConceptScore W3133787102C72958200 @default.
• W3133787102 hasConceptScore W3133787102C86803240 @default.
• W3133787102 hasLocation W31337871021 @default.
• W3133787102 hasLocation W31337871022 @default.
• W3133787102 hasLocation W31337871023 @default.
• W3133787102 hasOpenAccess W3133787102 @default.
• W3133787102 hasPrimaryLocation W31337871021 @default.
• W3133787102 hasRelatedWork W137234060 @default.
• W3133787102 hasRelatedWork W2051585809 @default.
• W3133787102 hasRelatedWork W2472137295 @default.
• W3133787102 hasRelatedWork W2591935376 @default.
• W3133787102 hasRelatedWork W2895704101 @default.
• W3133787102 hasRelatedWork W2950218343 @default.
• W3133787102 hasRelatedWork W3133787102 @default.
• W3133787102 hasRelatedWork W3165135927 @default.
• W3133787102 hasRelatedWork W3195837780 @default.
• W3133787102 hasRelatedWork W4385568880 @default.
• W3133787102 hasVolume "4" @default.
• W3133787102 isParatext "false" @default.
• W3133787102 isRetracted "false" @default.
• W3133787102 magId "3133787102" @default.
• W3133787102 workType "article" @default.