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• W3133808416 endingPage "106472" @default.
• W3133808416 startingPage "106472" @default.
• W3133808416 abstract "Chlordecone (CD), also known as Kepone, is an organochlorine insecticide that has been used in banana crops in the French West Indies. Due to long-term contamination of soils and water, the population is still exposed to CD. Exposure to CD in adulthood is associated with an increased risk of prostate cancer (PCa). We examined the transgenerational effects of CD on murine prostate tissue. We exposed pregnant Swiss mice to CD. The prostates from directly exposed (F1) and non-exposed (F3) male progeny were analyzed. We used immunofluorescence, RNA-seq and ChIP-seq techniques for the comprehensive analyses of chromatin states in prostate. We observed an increased prostatic intraepithelial neoplasia phenotype (PIN) in both F1 and F3 generations. Transcriptomic analysis in CD-derived F1 and F3 prostate using RNA-seq revealed that 970 genes in F1 and 218 in F3 genes were differentially expressed. The differentially expressed genes in both datasets could be clustered accordingly to common biological processes, “cell differentiation”, “developmental process”, “regulating of signaling”, suggesting that in both generations similar processes were perturbed. We detected that in both datasets several Hox genes were upregulated; in F1, the expression was detected mainly in Hoxb and Hoxd, and in F3, in Hoxa family genes. Using a larger number of biological replicates and RT-qPCR we showed that genes implicated in testosterone synthesis (Akr1b3, Cyp11a1, Cyp17a1, Srd5a1) were dramatically upregulated in PIN samples; Cyp19a1, converting testosterone to estradiol was elevated as well. We found a dramatic increase in Esr2 expression both in F1 and F3 prostates containing PIN. The PIN-containing samples have a strong increase in expression of self-renewal-related genes (Nanog, Tbx3, Sox2, Sox3, Rb1). We observed changes in liver, F1 CD-exposed males have an increased expression of genes related to DNA repair, matrix collagen and inflammation related pathways in F1 but not in F3 adult CD-derived liver. The changes in RNA transcription were associated with epigenetic changes. Specifically, we found a global increase in H3K4 trimethylation (H3K4me3) and a decrease in H3K27 trimethylation (H3K27me3) in prostate of F1 mice. ChIP-seq analysis showed that 129 regions in F1 and 240 in F3 acquired altered H3K4me3 occupancy in CD-derived prostate, including highest increase at several promoters of Hoxa family genes in both datasets. The alteration in H3K4me3 in both generations overlap 73 genes including genes involved in proliferation regulation, Tbx2, Stat3, Stat5a, Pou2f3 and homeobox genes Hoxa13, Hoxa9. Our data suggest that developmental exposure to CD leads to epigenetic changes in prostate tissue. The PIN containing samples showed evidence of implication in hormonal pathway and self-renewal gene expression that have the capacity to promote neoplasia in CD-exposed mice." @default.
• W3133808416 created "2021-03-15" @default.
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• W3133808416 date "2021-07-01" @default.
• W3133808416 modified "2023-10-12" @default.
• W3133808416 title "Developmental exposure to chlordecone induces transgenerational effects in somatic prostate tissue which are associated with epigenetic histone trimethylation changes" @default.
• W3133808416 cites W1821797507 @default.
• W3133808416 cites W1910049290 @default.
• W3133808416 cites W1955730995 @default.
• W3133808416 cites W1967648719 @default.
• W3133808416 cites W1977021269 @default.
• W3133808416 cites W1979594866 @default.
• W3133808416 cites W1985075299 @default.
• W3133808416 cites W1988295653 @default.
• W3133808416 cites W1988934407 @default.
• W3133808416 cites W1994579188 @default.
• W3133808416 cites W2000825526 @default.
• W3133808416 cites W2009906631 @default.
• W3133808416 cites W2011592069 @default.
• W3133808416 cites W2016835066 @default.
• W3133808416 cites W2026821106 @default.
• W3133808416 cites W2026835968 @default.
• W3133808416 cites W2028536804 @default.
• W3133808416 cites W2030197700 @default.
• W3133808416 cites W2035617569 @default.
• W3133808416 cites W2037818631 @default.
• W3133808416 cites W2048249977 @default.
• W3133808416 cites W2051854412 @default.
• W3133808416 cites W2055031610 @default.
• W3133808416 cites W2062840639 @default.
• W3133808416 cites W2063036839 @default.
• W3133808416 cites W2064850104 @default.
• W3133808416 cites W2067561829 @default.
• W3133808416 cites W2071213636 @default.
• W3133808416 cites W2071365780 @default.
• W3133808416 cites W2079360110 @default.
• W3133808416 cites W2092488497 @default.
• W3133808416 cites W2093321694 @default.
• W3133808416 cites W2093503215 @default.
• W3133808416 cites W2099054500 @default.
• W3133808416 cites W2100091900 @default.
• W3133808416 cites W2103410123 @default.
• W3133808416 cites W2109193598 @default.
• W3133808416 cites W2109469421 @default.
• W3133808416 cites W2109697470 @default.
• W3133808416 cites W2125000518 @default.
• W3133808416 cites W2127431151 @default.
• W3133808416 cites W2129487614 @default.
• W3133808416 cites W2136883443 @default.
• W3133808416 cites W2141569270 @default.
• W3133808416 cites W2146713203 @default.
• W3133808416 cites W2150550754 @default.
• W3133808416 cites W2153737103 @default.
• W3133808416 cites W2155725346 @default.
• W3133808416 cites W2164097156 @default.
• W3133808416 cites W2168245581 @default.
• W3133808416 cites W2169236261 @default.
• W3133808416 cites W2169604225 @default.
• W3133808416 cites W2178232667 @default.
• W3133808416 cites W2260392779 @default.
• W3133808416 cites W2520701010 @default.
• W3133808416 cites W2520906511 @default.
• W3133808416 cites W2601176113 @default.
• W3133808416 cites W2613739046 @default.
• W3133808416 cites W2741251162 @default.
• W3133808416 cites W2766568579 @default.
• W3133808416 cites W2789804929 @default.
• W3133808416 cites W2810492633 @default.
• W3133808416 cites W2810630532 @default.
• W3133808416 cites W2889502917 @default.
• W3133808416 cites W2891477819 @default.
• W3133808416 cites W2894735574 @default.
• W3133808416 cites W2902372981 @default.
• W3133808416 cites W2912086164 @default.
• W3133808416 cites W2944545583 @default.
• W3133808416 cites W2946012359 @default.
• W3133808416 cites W2946892839 @default.
• W3133808416 cites W2949885749 @default.
• W3133808416 cites W2961382565 @default.
• W3133808416 cites W2972661946 @default.
• W3133808416 cites W2992851114 @default.
• W3133808416 cites W300603418 @default.
• W3133808416 cites W3093352452 @default.
• W3133808416 cites W3099018540 @default.
• W3133808416 cites W785317484 @default.
• W3133808416 doi "https://doi.org/10.1016/j.envint.2021.106472" @default.
• W3133808416 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33711761" @default.
• W3133808416 hasPublicationYear "2021" @default.
• W3133808416 type Work @default.

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