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• W3133818498 abstract "Abstract The proper balance of hematopoietic stem cell (HSC) self-renewal and differentiation is critical for normal hematopoiesis and is disrupted in hematologic malignancy. Among regulators of HSC fate, transcription factors have a well-defined central role, and mutations promote malignant transformation. More recently, studies have illuminated the importance of posttranscriptional regulation by RNA-binding proteins (RBPs) in hematopoiesis and leukemia development. However, the RBPs involved and the breadth of regulation are only beginning to be elucidated. Furthermore, the intersection between posttranscriptional regulation and hematopoietic transcription factor function is poorly understood. Here, we studied the posttranscriptional regulation of RUNX1, a key hematopoietic transcription factor. Alternative polyadenylation (APA) of RUNX1 produces functionally antagonistic protein isoforms (RUNX1a vs RUNX1b/c) that mediate HSC self-renewal vs differentiation, an RNA-processing event that is dysregulated in malignancy. Consequently, RBPs that regulate this event directly contribute to healthy and aberrant hematopoiesis. We modeled RUNX1 APA using a split GFP minigene reporter and confirmed the sensitivity of our model to detect changes in RNA processing. We used this reporter in a clustered regularly interspaced short palindromic repeats (CRISPR) screen consisting of single guide RNAs exclusively targeting RBPs and uncovered HNRNPA1 and KHDRBS1 as antagonistic regulators of RUNX1a isoform generation. Overall, our study provides mechanistic insight into the posttranscriptional regulation of a key hematopoietic transcription factor and identifies RBPs that may have widespread and important functions in hematopoiesis." @default.
• W3133818498 created "2021-03-15" @default.
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• W3133818498 date "2021-03-03" @default.
• W3133818498 modified "2023-10-01" @default.
• W3133818498 title "A CRISPR RNA-binding protein screen reveals regulators of RUNX1 isoform generation" @default.
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• W3133818498 cites W1506037097 @default.
• W3133818498 cites W15248648 @default.
• W3133818498 cites W1534005970 @default.
• W3133818498 cites W1699218328 @default.
• W3133818498 cites W1749659233 @default.
• W3133818498 cites W1975454303 @default.
• W3133818498 cites W1975511102 @default.
• W3133818498 cites W1978135986 @default.
• W3133818498 cites W1980706177 @default.
• W3133818498 cites W1985267020 @default.
• W3133818498 cites W1989695798 @default.
• W3133818498 cites W1998460742 @default.
• W3133818498 cites W1999609473 @default.
• W3133818498 cites W2011965408 @default.
• W3133818498 cites W2016533650 @default.
• W3133818498 cites W2018721001 @default.
• W3133818498 cites W2022046147 @default.
• W3133818498 cites W2025187528 @default.
• W3133818498 cites W2027784918 @default.
• W3133818498 cites W2031743991 @default.
• W3133818498 cites W2036065307 @default.
• W3133818498 cites W2038921819 @default.
• W3133818498 cites W2044648543 @default.
• W3133818498 cites W2046697661 @default.
• W3133818498 cites W2050573038 @default.
• W3133818498 cites W2051641768 @default.
• W3133818498 cites W2061347183 @default.
• W3133818498 cites W2063151549 @default.
• W3133818498 cites W2063571420 @default.
• W3133818498 cites W2064813555 @default.
• W3133818498 cites W2067020273 @default.
• W3133818498 cites W2068180510 @default.
• W3133818498 cites W2068728784 @default.
• W3133818498 cites W2073889874 @default.
• W3133818498 cites W2076076392 @default.
• W3133818498 cites W2089242137 @default.
• W3133818498 cites W2090915512 @default.
• W3133818498 cites W2091242855 @default.
• W3133818498 cites W2091741479 @default.
• W3133818498 cites W2099915768 @default.
• W3133818498 cites W2101815618 @default.
• W3133818498 cites W2105545925 @default.
• W3133818498 cites W2108053190 @default.
• W3133818498 cites W2108264419 @default.
• W3133818498 cites W2111117771 @default.
• W3133818498 cites W2118031210 @default.
• W3133818498 cites W2119875500 @default.
• W3133818498 cites W2124515729 @default.
• W3133818498 cites W2129541995 @default.
• W3133818498 cites W2131769243 @default.
• W3133818498 cites W2133094898 @default.
• W3133818498 cites W2134907482 @default.
• W3133818498 cites W2151562469 @default.
• W3133818498 cites W2155432224 @default.
• W3133818498 cites W2161371146 @default.
• W3133818498 cites W2163939306 @default.
• W3133818498 cites W2167816004 @default.
• W3133818498 cites W2201689894 @default.
• W3133818498 cites W2329506014 @default.
• W3133818498 cites W2341469523 @default.
• W3133818498 cites W2469948286 @default.
• W3133818498 cites W2549890733 @default.
• W3133818498 cites W2564955822 @default.
• W3133818498 cites W2652154361 @default.
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• W3133818498 cites W2900119089 @default.
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• W3133818498 doi "https://doi.org/10.1182/bloodadvances.2020002090" @default.
• W3133818498 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7948294" @default.
• W3133818498 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33656539" @default.
• W3133818498 hasPublicationYear "2021" @default.
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