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• W3133924351 abstract "• The vapors obtained from different refill liquids for e-cigarettes exert different extent of cytotoxicity. • The condensates obtained from traditional cigarettes are always highly cytotoxic in the same experimental conditions. • Accurate evaluations for refill liquids will help to correlate toxicity to chemical composition. The electronic cigarettes mimic combustible cigarettes through a heating technology that vaporizes a refill liquid consisting of solvents, flavors, and nicotine. E-cigarettes are sometimes still used as a support for smoking cessation, even if in 2019 an acute lung injury outbreak occurred in the USA, affecting mainly adolescents and young adults, and was correlated to eCigs. Therefore, due to the lack of a definite knowledge about the mechanism(s) of refill liquid toxicity and considering that previous investigations gave controversial results, the aim of the present study was the cytotoxicity assessment of different refill liquids on human endothelial cells, evaluated by means of two different in vitro approaches, i.e. the resazurin and the LDH release assays. Our results clearly demonstrated that different refill liquids (6 samples) display different levels of cytotoxicity in our cellular model, although their cytotoxicity was always lower than that observed for the condensate obtained from traditional cigarettes (3 samples). These results suggest that accurate evaluations should be provided for refill liquids, in particular to correlate their toxicity to their chemical composition, with the final aim of obtaining useful information for the agencies involved in the regulation of their components." @default.
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• W3133924351 date "2021-01-01" @default.
• W3133924351 modified "2023-10-01" @default.
• W3133924351 title "Refill liquids for electronic cigarettes display peculiar toxicity on human endothelial cells" @default.
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• W3133924351 doi "https://doi.org/10.1016/j.toxrep.2021.02.021" @default.
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