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- W3134029596 abstract "Exosomes that contain various signaling molecules, such as proteins, nucleotides, metabolites, and lipids, are important for intercellular communication. Dendritic cells (DC) are central regulators of anti-tumor immunity but can be suppressed by tumor-derived exosomes (TDEs) in the tumor microenvironment. Here, we describe a step-by-step protocol for TDE isolation and evaluation of TDEs on DCs both in vitro and ex vivo with high repeatability. This approach is useful for the interrogating TDE-DC interactions and identification of novel immune regulators. For complete details on the use and execution of this protocol, please refer to Yin et al. (2020)." @default.
- W3134029596 created "2021-03-15" @default.
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- W3134029596 date "2021-03-01" @default.
- W3134029596 modified "2023-10-04" @default.
- W3134029596 title "In vitro and ex vivo evaluation of tumor-derived exosome-induced dendritic cell dysfunction in mouse" @default.
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- W3134029596 doi "https://doi.org/10.1016/j.xpro.2021.100361" @default.
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