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- W3134087127 abstract "Intrinsic primary afferent neurons (IPANs) enable the gut to manifest reflexes in the absence of CNS input. PKG1α is selectively expressed in a subset of neurons in dorsal root ganglia (DRG) and has been linked to nociception and long-term hyperexcitability.We used immunoblotting, immunocytochemistry, and in vitro assays of IPAN-dependent enteric functions to test hypotheses that subsets of primary neurons of the ENS and DRG share a reliance on PKG1α expression.PKG1α immunoreactivity was demonstrated in immunoblots from isolated myenteric ganglia. PKG1α, but not PKG1β, immunoreactivity, was coincident with that of neuronal markers (HuC/D; β3-tubulin) in both enteric plexuses. PKG1α immunoreactivity also co-localized with the immunoreactivities of the IPAN markers, calbindin (100%; myenteric plexus) and cytoplasmic NeuN (98 ± 1% submucosal plexus). CGRP-immunoreactive DRG neurons, identified as visceral afferents by retrograde transport, were PKG1α-immunoreactive. We used intraluminal cholera toxin to determine whether PKG1α was necessary to enable stimulation of the mucosa to activate Fos in enteric neurons. Tetrodotoxin (1.0 µM), low Ca2+ /high Mg2+ media, and the PKG inhibitor, N46 (100 µM), all inhibited Fos activation in myenteric neurons. N46 also concentration dependently inhibited peristaltic reflexes in isolated preparations of distal colon (IC50 = 83.3 ± 1.3 µM).These data suggest that PKG1α is present and functionally important in IPANs and visceral afferent nociceptive neurons." @default.
- W3134087127 created "2021-03-15" @default.
- W3134087127 creator A5007323247 @default.
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- W3134087127 creator A5040278204 @default.
- W3134087127 creator A5054898178 @default.
- W3134087127 creator A5067760423 @default.
- W3134087127 date "2021-03-03" @default.
- W3134087127 modified "2023-10-18" @default.
- W3134087127 title "The α isoform of cGMP‐dependent protein kinase 1 (PKG1α) is expressed and functionally important in intrinsic primary afferent neurons of the guinea pig enteric nervous system" @default.
- W3134087127 cites W1508341207 @default.
- W3134087127 cites W150998259 @default.
- W3134087127 cites W1547288529 @default.
- W3134087127 cites W1551307191 @default.
- W3134087127 cites W1576727246 @default.
- W3134087127 cites W1593049722 @default.
- W3134087127 cites W1662913097 @default.
- W3134087127 cites W1770296628 @default.
- W3134087127 cites W1831892029 @default.
- W3134087127 cites W1839541123 @default.
- W3134087127 cites W1868922166 @default.
- W3134087127 cites W1885514854 @default.
- W3134087127 cites W1898760913 @default.
- W3134087127 cites W1907864108 @default.
- W3134087127 cites W1964023408 @default.
- W3134087127 cites W1964897036 @default.
- W3134087127 cites W1965420213 @default.
- W3134087127 cites W1966362949 @default.
- W3134087127 cites W1970757039 @default.
- W3134087127 cites W1972348334 @default.
- W3134087127 cites W1975183047 @default.
- W3134087127 cites W1975748795 @default.
- W3134087127 cites W1976200262 @default.
- W3134087127 cites W1976901921 @default.
- W3134087127 cites W1979675387 @default.
- W3134087127 cites W1980194810 @default.
- W3134087127 cites W1980588138 @default.
- W3134087127 cites W1981811756 @default.
- W3134087127 cites W1984711986 @default.
- W3134087127 cites W1989867082 @default.
- W3134087127 cites W1990736267 @default.
- W3134087127 cites W1991175004 @default.
- W3134087127 cites W1991772933 @default.
- W3134087127 cites W1991948807 @default.
- W3134087127 cites W1993725963 @default.
- W3134087127 cites W2000953793 @default.
- W3134087127 cites W2002297978 @default.
- W3134087127 cites W2002620715 @default.
- W3134087127 cites W2003745741 @default.
- W3134087127 cites W2004258846 @default.
- W3134087127 cites W2006709336 @default.
- W3134087127 cites W2007640952 @default.
- W3134087127 cites W2007944782 @default.
- W3134087127 cites W2008465842 @default.
- W3134087127 cites W2012929011 @default.
- W3134087127 cites W2013368152 @default.
- W3134087127 cites W2016181711 @default.
- W3134087127 cites W2018289835 @default.
- W3134087127 cites W2021640479 @default.
- W3134087127 cites W2021955238 @default.
- W3134087127 cites W2022599897 @default.
- W3134087127 cites W2027918140 @default.
- W3134087127 cites W2028694253 @default.
- W3134087127 cites W2031129058 @default.
- W3134087127 cites W2036907717 @default.
- W3134087127 cites W2037344075 @default.
- W3134087127 cites W2039255033 @default.
- W3134087127 cites W2040037358 @default.
- W3134087127 cites W2040318996 @default.
- W3134087127 cites W2041091955 @default.
- W3134087127 cites W2046839540 @default.
- W3134087127 cites W2049314578 @default.
- W3134087127 cites W2050174256 @default.
- W3134087127 cites W2051373174 @default.
- W3134087127 cites W2052826002 @default.
- W3134087127 cites W2054944339 @default.
- W3134087127 cites W2055753133 @default.
- W3134087127 cites W2056608204 @default.
- W3134087127 cites W2057090803 @default.
- W3134087127 cites W2058878920 @default.
- W3134087127 cites W2059679962 @default.
- W3134087127 cites W2063601395 @default.
- W3134087127 cites W2067042083 @default.
- W3134087127 cites W2075715972 @default.
- W3134087127 cites W2083663422 @default.
- W3134087127 cites W2083787933 @default.
- W3134087127 cites W2085052553 @default.
- W3134087127 cites W2086614405 @default.
- W3134087127 cites W2087022299 @default.
- W3134087127 cites W2091746433 @default.
- W3134087127 cites W2092450619 @default.
- W3134087127 cites W2095658396 @default.
- W3134087127 cites W2098976206 @default.
- W3134087127 cites W2100837269 @default.
- W3134087127 cites W2104428938 @default.
- W3134087127 cites W2107044225 @default.
- W3134087127 cites W2109991720 @default.
- W3134087127 cites W2113426565 @default.
- W3134087127 cites W2116064885 @default.