FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3134429488> ?p ?o ?g. }

• W3134429488 abstract "The serine protease prostasin (Prss8) is expressed in the distal tubule and stimulates proteolytic activation of the epithelial sodium channel (ENaC) in co-expression experiments in vitro. The aim of this study was to explore the role of prostasin in proteolytic ENaC activation in the kidney in vivo.We used genetically modified knockin mice carrying a Prss8 mutation abolishing proteolytic activity (Prss8-S238A) or a mutation leading to a zymogen-locked state (Prss8-R44Q). Mice were challenged with low sodium diet and diuretics. Regulation of ENaC activity by Prss8-S238A and Prss8-R44Q was studied in vitro using the Xenopus laevis oocyte expression system.Co-expression of murine ENaC with Prss8-wt or Prss8-S238A in oocytes caused maximal proteolytic ENaC activation, whereas ENaC was activated only partially in oocytes co-expressing Prss8-R44Q. This was paralleled by a reduced proteolytic activity at the cell surface of Prss8-R44Q expressing oocytes. Sodium conservation under low sodium diet was preserved in Prss8-S238A and Prss8-R44Q mice but with higher plasma aldosterone concentrations in Prss8-R44Q mice. Treatment with the ENaC inhibitor triamterene over four days was tolerated in Prss8-wt and Prss8-S238A mice, whereas Prss8-R44Q mice developed salt wasting and severe weight loss associated with hyperkalemia and acidosis consistent with impaired ENaC function and renal failure.Unlike proteolytically inactive Prss8-S238A, zymogen-locked Prss8-R44Q produces incomplete proteolytic ENaC activation in vitro and causes a severe renal phenotype in mice treated with the ENaC inhibitor triamterene. This indicates that Prss8 plays a role in proteolytic ENaC activation and renal function independent of its proteolytic activity." @default.
• W3134429488 created "2021-03-15" @default.
• W3134429488 creator A5006083279 @default.
• W3134429488 creator A5012133037 @default.
• W3134429488 creator A5013839934 @default.
• W3134429488 creator A5015092347 @default.
• W3134429488 creator A5015526689 @default.
• W3134429488 creator A5029289418 @default.
• W3134429488 creator A5030304410 @default.
• W3134429488 creator A5051985691 @default.
• W3134429488 creator A5058284896 @default.
• W3134429488 creator A5080306130 @default.
• W3134429488 creator A5084711674 @default.
• W3134429488 creator A5087063047 @default.
• W3134429488 date "2021-03-11" @default.
• W3134429488 modified "2023-10-11" @default.
• W3134429488 title "Zymogen‐locked mutant prostasin (Prss8) leads to incomplete proteolytic activation of the epithelial sodium channel (ENaC) and severely compromises triamterene tolerance in mice" @default.
• W3134429488 cites W1486524131 @default.
• W3134429488 cites W1585031586 @default.
• W3134429488 cites W1905022879 @default.
• W3134429488 cites W1983019832 @default.
• W3134429488 cites W1986962105 @default.
• W3134429488 cites W1997027413 @default.
• W3134429488 cites W2014538976 @default.
• W3134429488 cites W2018202678 @default.
• W3134429488 cites W2020056111 @default.
• W3134429488 cites W2021407435 @default.
• W3134429488 cites W2026867156 @default.
• W3134429488 cites W2041965973 @default.
• W3134429488 cites W2057457864 @default.
• W3134429488 cites W2065311008 @default.
• W3134429488 cites W2079851211 @default.
• W3134429488 cites W2087732866 @default.
• W3134429488 cites W2098964013 @default.
• W3134429488 cites W2099651526 @default.
• W3134429488 cites W2104630290 @default.
• W3134429488 cites W2109328478 @default.
• W3134429488 cites W2111410841 @default.
• W3134429488 cites W2133813097 @default.
• W3134429488 cites W2140830639 @default.
• W3134429488 cites W2144724313 @default.
• W3134429488 cites W2149949073 @default.
• W3134429488 cites W2239922633 @default.
• W3134429488 cites W2276153739 @default.
• W3134429488 cites W2309246622 @default.
• W3134429488 cites W2322828581 @default.
• W3134429488 cites W2366975044 @default.
• W3134429488 cites W2472204272 @default.
• W3134429488 cites W2506664189 @default.
• W3134429488 cites W2589803788 @default.
• W3134429488 cites W2614256117 @default.
• W3134429488 cites W2750899741 @default.
• W3134429488 cites W2785056357 @default.
• W3134429488 cites W2788915784 @default.
• W3134429488 cites W2791873059 @default.
• W3134429488 cites W2792059913 @default.
• W3134429488 cites W2795687816 @default.
• W3134429488 cites W2795722194 @default.
• W3134429488 cites W2937109513 @default.
• W3134429488 cites W2978006392 @default.
• W3134429488 cites W3032680730 @default.
• W3134429488 cites W3085957592 @default.
• W3134429488 cites W3126746158 @default.
• W3134429488 cites W3127320323 @default.
• W3134429488 cites W4210279082 @default.
• W3134429488 cites W4236415234 @default.
• W3134429488 doi "https://doi.org/10.1111/apha.13640" @default.
• W3134429488 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8159854" @default.
• W3134429488 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33650216" @default.
• W3134429488 hasPublicationYear "2021" @default.
• W3134429488 type Work @default.
• W3134429488 sameAs 3134429488 @default.
• W3134429488 citedByCount "14" @default.
• W3134429488 countsByYear W31344294882021 @default.
• W3134429488 countsByYear W31344294882022 @default.
• W3134429488 crossrefType "journal-article" @default.
• W3134429488 hasAuthorship W3134429488A5006083279 @default.
• W3134429488 hasAuthorship W3134429488A5012133037 @default.
• W3134429488 hasAuthorship W3134429488A5013839934 @default.
• W3134429488 hasAuthorship W3134429488A5015092347 @default.
• W3134429488 hasAuthorship W3134429488A5015526689 @default.
• W3134429488 hasAuthorship W3134429488A5029289418 @default.
• W3134429488 hasAuthorship W3134429488A5030304410 @default.
• W3134429488 hasAuthorship W3134429488A5051985691 @default.
• W3134429488 hasAuthorship W3134429488A5058284896 @default.
• W3134429488 hasAuthorship W3134429488A5080306130 @default.
• W3134429488 hasAuthorship W3134429488A5084711674 @default.
• W3134429488 hasAuthorship W3134429488A5087063047 @default.
• W3134429488 hasBestOaLocation W31344294882 @default.
• W3134429488 hasConcept C126322002 @default.
• W3134429488 hasConcept C134018914 @default.
• W3134429488 hasConcept C178790620 @default.
• W3134429488 hasConcept C181199279 @default.
• W3134429488 hasConcept C185592680 @default.
• W3134429488 hasConcept C2777265534 @default.
• W3134429488 hasConcept C2777622974 @default.
• W3134429488 hasConcept C2778525890 @default.
• W3134429488 hasConcept C2778775115 @default.
• W3134429488 hasConcept C2780361556 @default.
• W3134429488 hasConcept C537181965 @default.

• next