FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3134466734> ?p ?o ?g. }

• W3134466734 endingPage "1342" @default.
• W3134466734 startingPage "1332" @default.
• W3134466734 abstract "Pyruvate:ferredoxin oxidoreductase (PFOR) is a key enzyme in bacterial anaerobic metabolism. Since a low-potential ferredoxin (Fd2- ) is used as electron carrier, PFOR allows for hydrogen evolution during heterotrophic growth as well as pyruvate synthesis during lithoautotrophic growth. The thermophilic acetogenic model bacterium Thermoanaerobacter kivui can use both modes of lifestyle, but the nature of the PFOR in this organism was previously unestablished. Here, we have isolated PFOR to apparent homogeneity from cells grown on glucose. Peptide mass fingerprinting revealed that it is encoded by pfor1. PFOR uses pyruvate as an electron donor and methylene blue (1.8 U·mg-1 ) and ferredoxin (Fd; 27.2 U·mg-1 ) as electron acceptors, and the reaction is dependent on thiamine pyrophosphate, pyruvate, coenzyme A, and Fd. The pH and temperature optima were 7.5 and 66 °C, respectively. We detected 13.6 mol of iron·mol of protein-1 , consistent with the presence of three predicted [4Fe-4S] clusters. The ability to provide reduced Fd makes PFOR an interesting auxiliary enzyme for enzyme assays. To simplify and speed up the purification procedure, we established a protocol for homologous protein production in T. kivui. Therefore, pfor1 was cloned and expressed in T. kivui and the encoded protein containing a genetically engineered His-tag was purified in only two steps to apparent homogeneity. The homologously produced PFOR1 had the same properties as the enzyme from T. kivui. The enzyme can be used as auxiliary enzyme in enzymatic assays that require reduced Fd as electron donor, such as electron-bifurcating enzymes, to keep a constant level of reduced Fd." @default.
• W3134466734 created "2021-03-15" @default.
• W3134466734 creator A5000803622 @default.
• W3134466734 creator A5021320642 @default.
• W3134466734 creator A5026566683 @default.
• W3134466734 creator A5061802621 @default.
• W3134466734 date "2021-04-04" @default.
• W3134466734 modified "2023-10-18" @default.
• W3134466734 title "The pyruvate:ferredoxin oxidoreductase of the thermophilic acetogen, <i>Thermoanaerobacter kivui</i>" @default.
• W3134466734 cites W1538196126 @default.
• W3134466734 cites W1564565358 @default.
• W3134466734 cites W1766118319 @default.
• W3134466734 cites W1789242594 @default.
• W3134466734 cites W1819850013 @default.
• W3134466734 cites W1893665289 @default.
• W3134466734 cites W1922922362 @default.
• W3134466734 cites W1969383181 @default.
• W3134466734 cites W1970604668 @default.
• W3134466734 cites W1973002206 @default.
• W3134466734 cites W1983902820 @default.
• W3134466734 cites W1985469540 @default.
• W3134466734 cites W1996039824 @default.
• W3134466734 cites W1996891655 @default.
• W3134466734 cites W1998048338 @default.
• W3134466734 cites W2023913818 @default.
• W3134466734 cites W2023952564 @default.
• W3134466734 cites W2035252706 @default.
• W3134466734 cites W2037705194 @default.
• W3134466734 cites W2038385241 @default.
• W3134466734 cites W2045802273 @default.
• W3134466734 cites W2046287787 @default.
• W3134466734 cites W2054457594 @default.
• W3134466734 cites W2057704466 @default.
• W3134466734 cites W2058752397 @default.
• W3134466734 cites W2070730177 @default.
• W3134466734 cites W2077787127 @default.
• W3134466734 cites W2085545931 @default.
• W3134466734 cites W2087653172 @default.
• W3134466734 cites W2090432387 @default.
• W3134466734 cites W2092638302 @default.
• W3134466734 cites W2118747939 @default.
• W3134466734 cites W2124820804 @default.
• W3134466734 cites W2125611981 @default.
• W3134466734 cites W2128148887 @default.
• W3134466734 cites W2139935752 @default.
• W3134466734 cites W2140282132 @default.
• W3134466734 cites W2152653245 @default.
• W3134466734 cites W2155013220 @default.
• W3134466734 cites W2158178789 @default.
• W3134466734 cites W2172371128 @default.
• W3134466734 cites W2384560189 @default.
• W3134466734 cites W2437125234 @default.
• W3134466734 cites W2557496587 @default.
• W3134466734 cites W2769316169 @default.
• W3134466734 cites W2793758811 @default.
• W3134466734 cites W2800140874 @default.
• W3134466734 cites W2809137787 @default.
• W3134466734 cites W2891338980 @default.
• W3134466734 cites W2921348589 @default.
• W3134466734 cites W3084454696 @default.
• W3134466734 cites W4235583278 @default.
• W3134466734 cites W4245256434 @default.
• W3134466734 cites W4293247451 @default.
• W3134466734 doi "https://doi.org/10.1002/2211-5463.13136" @default.
• W3134466734 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8091585" @default.
• W3134466734 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33660937" @default.
• W3134466734 hasPublicationYear "2021" @default.
• W3134466734 type Work @default.
• W3134466734 sameAs 3134466734 @default.
• W3134466734 citedByCount "17" @default.
• W3134466734 countsByYear W31344667342021 @default.
• W3134466734 countsByYear W31344667342022 @default.
• W3134466734 countsByYear W31344667342023 @default.
• W3134466734 crossrefType "journal-article" @default.
• W3134466734 hasAuthorship W3134466734A5000803622 @default.
• W3134466734 hasAuthorship W3134466734A5021320642 @default.
• W3134466734 hasAuthorship W3134466734A5026566683 @default.
• W3134466734 hasAuthorship W3134466734A5061802621 @default.
• W3134466734 hasBestOaLocation W31344667341 @default.
• W3134466734 hasConcept C134621786 @default.
• W3134466734 hasConcept C181199279 @default.
• W3134466734 hasConcept C185592680 @default.
• W3134466734 hasConcept C197957613 @default.
• W3134466734 hasConcept C2779268744 @default.
• W3134466734 hasConcept C2780047943 @default.
• W3134466734 hasConcept C27911776 @default.
• W3134466734 hasConcept C55493867 @default.
• W3134466734 hasConcept C86803240 @default.
• W3134466734 hasConceptScore W3134466734C134621786 @default.
• W3134466734 hasConceptScore W3134466734C181199279 @default.
• W3134466734 hasConceptScore W3134466734C185592680 @default.
• W3134466734 hasConceptScore W3134466734C197957613 @default.
• W3134466734 hasConceptScore W3134466734C2779268744 @default.
• W3134466734 hasConceptScore W3134466734C2780047943 @default.
• W3134466734 hasConceptScore W3134466734C27911776 @default.
• W3134466734 hasConceptScore W3134466734C55493867 @default.
• W3134466734 hasConceptScore W3134466734C86803240 @default.
• W3134466734 hasFunder F4320320879 @default.

• next