Matches in SemOpenAlex for { <https://semopenalex.org/work/W3134572533> ?p ?o ?g. }
- W3134572533 endingPage "107973" @default.
- W3134572533 startingPage "107973" @default.
- W3134572533 abstract "Salmonella infection represents a common foodborne zoonosis of public concern worldwide. Rapid and user-friendly nucleic acid-based detection methods like loop-mediated isothermal amplification (LAMP) can contribute to the improvement of food safety and prevention of foodborne illness. In the present study, a LAMP assay was established and validated as a specific and rapid tool for detecting Salmonella spp. in various food matrices. Primers targeted a region within the ttrRSBCA locus and enabled 100% inclusivity of the 88 tested Salmonella strains, while no false positive signal occurred in any of the 92 tested non-Salmonella strains. After 20-h enrichment of artificially contaminated minced meat and ready-to-eat salad, initial contamination levels of 1 CFU/25 g were detectable via LAMP. The establishment of a simplified DNA-isolation method, which was adapted for field applications, enabled similar detection probability after 20-h enrichment of artificially contaminated food samples. During validation, 82 variously processed food samples underwent comparative analysis by LAMP and the standard culture method. The LAMP assay was further validated by the investigation of reference material of unknown Salmonella contamination status. Results showed that the ttrRSBCA-based LAMP detection of Salmonella spp. is a suitable tool for rapid sample screening within 24 h including first enrichment. Additionally, all Salmonella isolates obtained during cultural examination of naturally contaminated samples were correctly identified by LAMP, thus making it a suitable confirmation method for official applications." @default.
- W3134572533 created "2021-03-15" @default.
- W3134572533 creator A5041366943 @default.
- W3134572533 creator A5052528682 @default.
- W3134572533 creator A5061145828 @default.
- W3134572533 creator A5061206464 @default.
- W3134572533 creator A5068925373 @default.
- W3134572533 creator A5087332297 @default.
- W3134572533 date "2021-08-01" @default.
- W3134572533 modified "2023-10-16" @default.
- W3134572533 title "Establishment and validation of a loop-mediated isothermal amplification (LAMP) assay targeting the ttrRSBCA locus for rapid detection of Salmonella spp. in food" @default.
- W3134572533 cites W127160596 @default.
- W3134572533 cites W1507190501 @default.
- W3134572533 cites W1532394514 @default.
- W3134572533 cites W1540378334 @default.
- W3134572533 cites W1804759123 @default.
- W3134572533 cites W1896186489 @default.
- W3134572533 cites W1925736715 @default.
- W3134572533 cites W1973207608 @default.
- W3134572533 cites W1988147778 @default.
- W3134572533 cites W2000483782 @default.
- W3134572533 cites W2002838794 @default.
- W3134572533 cites W2003783627 @default.
- W3134572533 cites W2031786732 @default.
- W3134572533 cites W2037171557 @default.
- W3134572533 cites W2040906381 @default.
- W3134572533 cites W2052597392 @default.
- W3134572533 cites W2056246131 @default.
- W3134572533 cites W2063820673 @default.
- W3134572533 cites W2098210396 @default.
- W3134572533 cites W2101997581 @default.
- W3134572533 cites W2102723475 @default.
- W3134572533 cites W2105275554 @default.
- W3134572533 cites W2115492124 @default.
- W3134572533 cites W2136034879 @default.
- W3134572533 cites W2137422114 @default.
- W3134572533 cites W2151996078 @default.
- W3134572533 cites W2159739018 @default.
- W3134572533 cites W2216385734 @default.
- W3134572533 cites W2329512817 @default.
- W3134572533 cites W2394602380 @default.
- W3134572533 cites W2745482070 @default.
- W3134572533 cites W2791487897 @default.
- W3134572533 cites W2800371040 @default.
- W3134572533 cites W2807932594 @default.
- W3134572533 cites W2898317908 @default.
- W3134572533 cites W2936776427 @default.
- W3134572533 cites W2947344110 @default.
- W3134572533 cites W2975535544 @default.
- W3134572533 cites W4243575454 @default.
- W3134572533 doi "https://doi.org/10.1016/j.foodcont.2021.107973" @default.
- W3134572533 hasPublicationYear "2021" @default.
- W3134572533 type Work @default.
- W3134572533 sameAs 3134572533 @default.
- W3134572533 citedByCount "10" @default.
- W3134572533 countsByYear W31345725332021 @default.
- W3134572533 countsByYear W31345725332022 @default.
- W3134572533 countsByYear W31345725332023 @default.
- W3134572533 crossrefType "journal-article" @default.
- W3134572533 hasAuthorship W3134572533A5041366943 @default.
- W3134572533 hasAuthorship W3134572533A5052528682 @default.
- W3134572533 hasAuthorship W3134572533A5061145828 @default.
- W3134572533 hasAuthorship W3134572533A5061206464 @default.
- W3134572533 hasAuthorship W3134572533A5068925373 @default.
- W3134572533 hasAuthorship W3134572533A5087332297 @default.
- W3134572533 hasBestOaLocation W31345725331 @default.
- W3134572533 hasConcept C112570922 @default.
- W3134572533 hasConcept C18903297 @default.
- W3134572533 hasConcept C24107716 @default.
- W3134572533 hasConcept C2781065037 @default.
- W3134572533 hasConcept C2909271431 @default.
- W3134572533 hasConcept C31903555 @default.
- W3134572533 hasConcept C34135077 @default.
- W3134572533 hasConcept C516717267 @default.
- W3134572533 hasConcept C523546767 @default.
- W3134572533 hasConcept C54355233 @default.
- W3134572533 hasConcept C552990157 @default.
- W3134572533 hasConcept C58684123 @default.
- W3134572533 hasConcept C60635243 @default.
- W3134572533 hasConcept C86803240 @default.
- W3134572533 hasConcept C89423630 @default.
- W3134572533 hasConceptScore W3134572533C112570922 @default.
- W3134572533 hasConceptScore W3134572533C18903297 @default.
- W3134572533 hasConceptScore W3134572533C24107716 @default.
- W3134572533 hasConceptScore W3134572533C2781065037 @default.
- W3134572533 hasConceptScore W3134572533C2909271431 @default.
- W3134572533 hasConceptScore W3134572533C31903555 @default.
- W3134572533 hasConceptScore W3134572533C34135077 @default.
- W3134572533 hasConceptScore W3134572533C516717267 @default.
- W3134572533 hasConceptScore W3134572533C523546767 @default.
- W3134572533 hasConceptScore W3134572533C54355233 @default.
- W3134572533 hasConceptScore W3134572533C552990157 @default.
- W3134572533 hasConceptScore W3134572533C58684123 @default.
- W3134572533 hasConceptScore W3134572533C60635243 @default.
- W3134572533 hasConceptScore W3134572533C86803240 @default.
- W3134572533 hasConceptScore W3134572533C89423630 @default.
- W3134572533 hasLocation W31345725331 @default.
- W3134572533 hasOpenAccess W3134572533 @default.