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• W3134600650 endingPage "2569" @default.
• W3134600650 startingPage "2569" @default.
• W3134600650 abstract "Tiger nut (Cyperus esculentus), a perennial C4 plant of the Cyperaceae family, is an unconventional crop that is distinguished by its oil-rich tubers, which also possesses the advantages of strong resistance, wide adaptability, short life periods, and large biomass. To facilitate studies on gene expression in this species, we identified and validated a series of reference genes (RGs) based on transcriptome data, which can be employed as internal controls for qRT-PCR analysis in tiger nut. Fourteen putative candidate RGs were identified and evaluated across nine different tissues of two cultivars, and the RGs were analyzed using three different algorithms (geNorm, NormFinder, and BestKeeper). The stability rankings of the candidate RGs were merged into consensus lists with RankAggreg. For the below-ground storage organ of tiger nut, the optimal RGs were TUB4 and UCE2 in different developmental stages of tubers. UCE2 and UBL5 were the most stably expressed RGs among all tissues, while Rubisco and PGK exhibited the lowest expression stability. UCE2, UBL5 and Rubisco were compared to normalize the expression levels of the caleosin (CLO) and diacylglycerol acyltransferase 2-2 (DGAT2-2) genes across the same tissues. Our results showed that the RGs identified in this study, which exhibit more uniform expression patterns, may be utilized for the normalization of qRT-PCR results, promoting further research on gene expression in various tissues of tiger nut." @default.
• W3134600650 created "2021-03-15" @default.
• W3134600650 creator A5005199544 @default.
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• W3134600650 creator A5050739400 @default.
• W3134600650 date "2021-03-04" @default.
• W3134600650 modified "2023-10-16" @default.
• W3134600650 title "Selection and Validation of Reference Genes for qRT-PCR Analysis in the Oil-Rich Tuber Crop Tiger Nut (Cyperus esculentus) Based on Transcriptome Data" @default.
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• W3134600650 doi "https://doi.org/10.3390/ijms22052569" @default.
• W3134600650 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7961719" @default.
• W3134600650 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33806437" @default.
• W3134600650 hasPublicationYear "2021" @default.
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