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• W313463622 abstract "Terminal restriction fragment length polymorphism (T-RFLP) analysis of PCR-amplified genes is a widely used fingerprinting technique in composting systems. This analysis is based on the restriction endonuclease digestion of fluorescently end-labeled PCR products. The digested product is mixed with a DNA size standard, itself labeled with a distinct fluorescent dye, and the fragments are then separated by capillary or gel electrophoresis using an automated sequencer. Upon analysis, only the terminal end-labeled restriction fragments are detected. An electropherogram is produced, which shows a profile of compost microbial community as a series of peaks of varying height. This technique has also been effectively used in the exploration of complex microbial environments and in the study of bacterial, archaeal, and eukaryal populations in natural habitats." @default.
• W313463622 created "2016-06-24" @default.
• W313463622 creator A5074550587 @default.
• W313463622 date "2009-10-25" @default.
• W313463622 modified "2023-09-23" @default.
• W313463622 title "Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis to Assess Microbial Community Structure in Compost Systems" @default.
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• W313463622 doi "https://doi.org/10.1007/978-1-60761-439-5_6" @default.
• W313463622 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19882281" @default.
• W313463622 hasPublicationYear "2009" @default.
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