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- W3134710249 abstract "Recent advances in synthetic biology and protein engineering have increased the number of allosteric transcription factors used to regulate independent promoters. These developments represent an important increase in our biological computing capacity, which will enable us to construct more sophisticated genetic programs for a broad range of biological technologies. However, the majority of these transcription factors are represented by the repressor phenotype (BUFFER), and require layered inversion to confer the antithetical logical function (NOT), requiring additional biological resources. Moreover, these engineered transcription factors typically utilize native ligand binding functions paired with alternate DNA binding functions. In this study, we have advanced the state-of-the-art by engineering and redesigning the PurR topology (a native antirepressor) to be responsive to caffeine, while mitigating responsiveness to the native ligand hypoxanthine-i.e., a deamination product of the input molecule adenine. Importantly, the resulting caffeine responsive transcription factors are not antagonized by the native ligand hypoxanthine. In addition, we conferred alternate DNA binding to the caffeine antirepressors, and to the PurR scaffold, creating 38 new transcription factors that are congruent with our current transcriptional programming structure. Finally, we leveraged this system of transcription factors to create integrated NOR logic and related feedback operations. This study represents the first example of a system of transcription factors (antirepressors) in which both the ligand binding site and the DNA binding functions were successfully engineered in tandem." @default.
- W3134710249 created "2021-03-15" @default.
- W3134710249 creator A5051928700 @default.
- W3134710249 creator A5080076293 @default.
- W3134710249 date "2021-03-09" @default.
- W3134710249 modified "2023-10-17" @default.
- W3134710249 title "Engineering Alternate Ligand Recognition in the PurR Topology: A System of Novel Caffeine Biosensing Transcriptional Antirepressors" @default.
- W3134710249 cites W118113730 @default.
- W3134710249 cites W1501766320 @default.
- W3134710249 cites W1511813894 @default.
- W3134710249 cites W1577834808 @default.
- W3134710249 cites W1815546356 @default.
- W3134710249 cites W1893143839 @default.
- W3134710249 cites W1963204303 @default.
- W3134710249 cites W1967013096 @default.
- W3134710249 cites W1968951383 @default.
- W3134710249 cites W1975993854 @default.
- W3134710249 cites W1980348663 @default.
- W3134710249 cites W1987063642 @default.
- W3134710249 cites W1994028898 @default.
- W3134710249 cites W1996644443 @default.
- W3134710249 cites W2005362919 @default.
- W3134710249 cites W2007477992 @default.
- W3134710249 cites W2010352258 @default.
- W3134710249 cites W2013334155 @default.
- W3134710249 cites W2014058981 @default.
- W3134710249 cites W2016099222 @default.
- W3134710249 cites W2017810575 @default.
- W3134710249 cites W2037735567 @default.
- W3134710249 cites W2037913229 @default.
- W3134710249 cites W2038701138 @default.
- W3134710249 cites W2045442804 @default.
- W3134710249 cites W2054117348 @default.
- W3134710249 cites W2055437194 @default.
- W3134710249 cites W2061620911 @default.
- W3134710249 cites W2062147399 @default.
- W3134710249 cites W2066825888 @default.
- W3134710249 cites W2072920810 @default.
- W3134710249 cites W2073265884 @default.
- W3134710249 cites W2073588416 @default.
- W3134710249 cites W2086235515 @default.
- W3134710249 cites W2087504899 @default.
- W3134710249 cites W2089901247 @default.
- W3134710249 cites W2100987592 @default.
- W3134710249 cites W2102300623 @default.
- W3134710249 cites W2105183598 @default.
- W3134710249 cites W2106231811 @default.
- W3134710249 cites W2111686506 @default.
- W3134710249 cites W2118464978 @default.
- W3134710249 cites W2132629607 @default.
- W3134710249 cites W2132940078 @default.
- W3134710249 cites W2134220523 @default.
- W3134710249 cites W2134508367 @default.
- W3134710249 cites W2135774614 @default.
- W3134710249 cites W2149981735 @default.
- W3134710249 cites W2158284529 @default.
- W3134710249 cites W2161829824 @default.
- W3134710249 cites W2174688478 @default.
- W3134710249 cites W2192893656 @default.
- W3134710249 cites W2260617737 @default.
- W3134710249 cites W2315893683 @default.
- W3134710249 cites W2319535076 @default.
- W3134710249 cites W2330245166 @default.
- W3134710249 cites W2335483154 @default.
- W3134710249 cites W2343741934 @default.
- W3134710249 cites W2345594933 @default.
- W3134710249 cites W2494704835 @default.
- W3134710249 cites W2515895007 @default.
- W3134710249 cites W2557496587 @default.
- W3134710249 cites W2559919850 @default.
- W3134710249 cites W2561201681 @default.
- W3134710249 cites W2584662239 @default.
- W3134710249 cites W2604210114 @default.
- W3134710249 cites W2605053716 @default.
- W3134710249 cites W2606538969 @default.
- W3134710249 cites W2617511364 @default.
- W3134710249 cites W2618047787 @default.
- W3134710249 cites W2618570363 @default.
- W3134710249 cites W2734702993 @default.
- W3134710249 cites W2764219046 @default.
- W3134710249 cites W2769650585 @default.
- W3134710249 cites W2790208523 @default.
- W3134710249 cites W2803662842 @default.
- W3134710249 cites W2805607426 @default.
- W3134710249 cites W2806394571 @default.
- W3134710249 cites W2885322683 @default.
- W3134710249 cites W2885994665 @default.
- W3134710249 cites W2894961565 @default.
- W3134710249 cites W2900498998 @default.
- W3134710249 cites W2904629875 @default.
- W3134710249 cites W2906891182 @default.
- W3134710249 cites W2912147854 @default.
- W3134710249 cites W2932346662 @default.
- W3134710249 cites W2950618382 @default.
- W3134710249 cites W2952545994 @default.
- W3134710249 cites W2974231003 @default.
- W3134710249 cites W2975708120 @default.
- W3134710249 cites W2980582516 @default.