FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3134711345> ?p ?o ?g. }

• W3134711345 endingPage "12959" @default.
• W3134711345 startingPage "12950" @default.
• W3134711345 abstract "Cryopreservation is a key step for current translational medicine including reproductive medicine, regenerative medicine, and cell therapy. However, it is challenging to preserve rare cells for practical applications due to the difficulty in handling low numbers of cells as well as the lack of highly efficient and biocompatible preservation protocols. Here, we developed an acoustic droplet vitrification method for high-efficiency handling and preservation of rare cells. By employing an acoustic droplet ejection device, we can encapsulate rare cells into water-in-air droplets with a volume from ∼pL to ∼nL and deposit these cell-containing droplets into a droplet array onto a substrate. By incorporating a cooling system into the droplet array substrate, we can vitrify hundreds to thousands of rare cells at an ultrafast speed (about ∼2 s) based on the high surface to volume ratio of the droplets. By optimizing this method with three different cell lines (a human lung cancer cell line, A549 cells, a human liver cell line, L02 cells, and a mouse embryonic fibroblast cell line, 3T3-L1 cells), we developed an effective protocol with excellent cell viability (e.g., >85% for days, >70% for months), proliferation, and adhesion. As a proof-of-concept application, we demonstrated that our method can rapidly handle and efficiently preserve rare cells, highlighting its broad applications in species diversity, basic research, and clinical medicine." @default.
• W3134711345 created "2021-03-15" @default.
• W3134711345 creator A5002572745 @default.
• W3134711345 creator A5009012652 @default.
• W3134711345 creator A5018861863 @default.
• W3134711345 creator A5022058493 @default.
• W3134711345 creator A5030222851 @default.
• W3134711345 creator A5035294718 @default.
• W3134711345 creator A5045365947 @default.
• W3134711345 creator A5075193593 @default.
• W3134711345 creator A5091818964 @default.
• W3134711345 date "2021-03-11" @default.
• W3134711345 modified "2023-09-24" @default.
• W3134711345 title "Acoustic Droplet Vitrification Method for High-Efficiency Preservation of Rare Cells" @default.
• W3134711345 cites W1983056774 @default.
• W3134711345 cites W1991862809 @default.
• W3134711345 cites W1998949551 @default.
• W3134711345 cites W2025706758 @default.
• W3134711345 cites W2031046488 @default.
• W3134711345 cites W2037770708 @default.
• W3134711345 cites W2041803366 @default.
• W3134711345 cites W2045496328 @default.
• W3134711345 cites W2060816289 @default.
• W3134711345 cites W2072900326 @default.
• W3134711345 cites W2099680256 @default.
• W3134711345 cites W2100324080 @default.
• W3134711345 cites W2113600976 @default.
• W3134711345 cites W2130338247 @default.
• W3134711345 cites W2131391649 @default.
• W3134711345 cites W2168807103 @default.
• W3134711345 cites W2169264000 @default.
• W3134711345 cites W2280988113 @default.
• W3134711345 cites W2524661281 @default.
• W3134711345 cites W2560186525 @default.
• W3134711345 cites W2580773030 @default.
• W3134711345 cites W2735770704 @default.
• W3134711345 cites W2770712959 @default.
• W3134711345 cites W2795505834 @default.
• W3134711345 cites W2903102775 @default.
• W3134711345 cites W2919732933 @default.
• W3134711345 cites W2924855856 @default.
• W3134711345 cites W2980354178 @default.
• W3134711345 cites W2998238775 @default.
• W3134711345 cites W3044869207 @default.
• W3134711345 doi "https://doi.org/10.1021/acsami.1c01452" @default.
• W3134711345 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33703892" @default.
• W3134711345 hasPublicationYear "2021" @default.
• W3134711345 type Work @default.
• W3134711345 sameAs 3134711345 @default.
• W3134711345 citedByCount "9" @default.
• W3134711345 countsByYear W31347113452021 @default.
• W3134711345 countsByYear W31347113452022 @default.
• W3134711345 countsByYear W31347113452023 @default.
• W3134711345 crossrefType "journal-article" @default.
• W3134711345 hasAuthorship W3134711345A5002572745 @default.
• W3134711345 hasAuthorship W3134711345A5009012652 @default.
• W3134711345 hasAuthorship W3134711345A5018861863 @default.
• W3134711345 hasAuthorship W3134711345A5022058493 @default.
• W3134711345 hasAuthorship W3134711345A5030222851 @default.
• W3134711345 hasAuthorship W3134711345A5035294718 @default.
• W3134711345 hasAuthorship W3134711345A5045365947 @default.
• W3134711345 hasAuthorship W3134711345A5075193593 @default.
• W3134711345 hasAuthorship W3134711345A5091818964 @default.
• W3134711345 hasConcept C104317684 @default.
• W3134711345 hasConcept C10854531 @default.
• W3134711345 hasConcept C136229726 @default.
• W3134711345 hasConcept C145103041 @default.
• W3134711345 hasConcept C16685009 @default.
• W3134711345 hasConcept C171250308 @default.
• W3134711345 hasConcept C179933525 @default.
• W3134711345 hasConcept C18903297 @default.
• W3134711345 hasConcept C192562407 @default.
• W3134711345 hasConcept C196843134 @default.
• W3134711345 hasConcept C2777289219 @default.
• W3134711345 hasConcept C2778022349 @default.
• W3134711345 hasConcept C28328180 @default.
• W3134711345 hasConcept C54355233 @default.
• W3134711345 hasConcept C55493867 @default.
• W3134711345 hasConcept C71924100 @default.
• W3134711345 hasConcept C81885089 @default.
• W3134711345 hasConcept C86803240 @default.
• W3134711345 hasConcept C95444343 @default.
• W3134711345 hasConceptScore W3134711345C104317684 @default.
• W3134711345 hasConceptScore W3134711345C10854531 @default.
• W3134711345 hasConceptScore W3134711345C136229726 @default.
• W3134711345 hasConceptScore W3134711345C145103041 @default.
• W3134711345 hasConceptScore W3134711345C16685009 @default.
• W3134711345 hasConceptScore W3134711345C171250308 @default.
• W3134711345 hasConceptScore W3134711345C179933525 @default.
• W3134711345 hasConceptScore W3134711345C18903297 @default.
• W3134711345 hasConceptScore W3134711345C192562407 @default.
• W3134711345 hasConceptScore W3134711345C196843134 @default.
• W3134711345 hasConceptScore W3134711345C2777289219 @default.
• W3134711345 hasConceptScore W3134711345C2778022349 @default.
• W3134711345 hasConceptScore W3134711345C28328180 @default.
• W3134711345 hasConceptScore W3134711345C54355233 @default.
• W3134711345 hasConceptScore W3134711345C55493867 @default.
• W3134711345 hasConceptScore W3134711345C71924100 @default.

• next