FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3134737776> ?p ?o ?g. }

• W3134737776 endingPage "102246" @default.
• W3134737776 startingPage "102246" @default.
• W3134737776 abstract "Abstract Human completely rely on food and nutrition supply to absorb enough lutein, which is an important antioxidant beneficial to health of eyes and heart. In plants, lycopene epsilon-cyclase (LCYE) is the key enzyme in lutein synthesis pathway, where lycopene is converted into δ-carotene, α-carotene and lutein. In this study, for the first time, we successfully cloned CvLCYE, the evolutionarily conserved lycopene cyclase in the genome of Chlorella vulgaris that is a green alga rich in lutein. Homology analysis showed that the amino acid sequence of CvLCYE protein is factually high identical with that of other aquatic green algae including Chlorella variabilis, Auxenochlorella protothecoides strain CS-41, Chromochloris zofingiensis and Chlamydomonas reinhardtii. Meanwhile, the UPGMA tree analysis of CvLCYE protein sequence showed the consistent results. Bioinformatic analysis of CvLCYE protein revealed a typical lycopene cyclase domain (Pfam05834) between the 64th–475th amino acid. The 3D structure prediction of CvLCYE protein displayed a structure of beta strand-alpha helix-beta strand, which is conserved in all known lycopene epsilon-cyclases. Subcellular localization tests demonstrated that mature CvLCYE-GFP fused protein localized to cytoplasm in Arabidopsis protoplasts. Importantly, targeted HPLC analysis on lycopene content are performed with Escherichia coli strain expressing CvLCYE, which displayed faded pink of consumption of lycopene, in comparison to strain expressing empty vector, which maintained pink because of accumulation of lycopene. HPLC data strongly suggested the capability of CvLCYE to catalyze lycopene. Furthermore, enzymatic activity of CvLCYE was also clearly verified in Chlamydomonas reinhardtii that is the model organism for algae research. Interestingly, we found that the lutein content in transgenic C. reinhardtii expressing CvLCYE increased by about 2.3 times than that of wild type strain. All these results provide insight into the positive role played by CvLCYE in lutein synthesis and lay a promising foundation for the molecular breeding of lutein metabolic pathway in Chlorella vulgaris and other algae." @default.
• W3134737776 created "2021-03-15" @default.
• W3134737776 creator A5015782442 @default.
• W3134737776 creator A5045504835 @default.
• W3134737776 creator A5072331448 @default.
• W3134737776 creator A5083534218 @default.
• W3134737776 creator A5089507879 @default.
• W3134737776 creator A5091038325 @default.
• W3134737776 date "2021-05-01" @default.
• W3134737776 modified "2023-10-18" @default.
• W3134737776 title "Molecular cloning and functional characterization of CvLCYE, a key enzyme in lutein synthesis pathway in Chlorella vulgaris" @default.
• W3134737776 cites W1563355533 @default.
• W3134737776 cites W1724257157 @default.
• W3134737776 cites W1930388675 @default.
• W3134737776 cites W1964068845 @default.
• W3134737776 cites W197263901 @default.
• W3134737776 cites W1997960966 @default.
• W3134737776 cites W2006195402 @default.
• W3134737776 cites W2021795499 @default.
• W3134737776 cites W2037045595 @default.
• W3134737776 cites W2038561546 @default.
• W3134737776 cites W2040218897 @default.
• W3134737776 cites W2048259679 @default.
• W3134737776 cites W2048522430 @default.
• W3134737776 cites W2059525285 @default.
• W3134737776 cites W2067011277 @default.
• W3134737776 cites W2068388071 @default.
• W3134737776 cites W2069704281 @default.
• W3134737776 cites W2074002921 @default.
• W3134737776 cites W2077270544 @default.
• W3134737776 cites W2078212216 @default.
• W3134737776 cites W2080823787 @default.
• W3134737776 cites W2083565938 @default.
• W3134737776 cites W2083843490 @default.
• W3134737776 cites W2099073103 @default.
• W3134737776 cites W2120807642 @default.
• W3134737776 cites W2121196417 @default.
• W3134737776 cites W2123550792 @default.
• W3134737776 cites W2123569863 @default.
• W3134737776 cites W2130051906 @default.
• W3134737776 cites W2139874678 @default.
• W3134737776 cites W2141863851 @default.
• W3134737776 cites W2142888309 @default.
• W3134737776 cites W2145186502 @default.
• W3134737776 cites W2152207030 @default.
• W3134737776 cites W2154429475 @default.
• W3134737776 cites W2162290777 @default.
• W3134737776 cites W2168984531 @default.
• W3134737776 cites W2333633447 @default.
• W3134737776 cites W2346290259 @default.
• W3134737776 cites W2415083619 @default.
• W3134737776 cites W2502225217 @default.
• W3134737776 cites W2518829223 @default.
• W3134737776 cites W2519671479 @default.
• W3134737776 cites W2610808603 @default.
• W3134737776 cites W2618662740 @default.
• W3134737776 cites W2729793328 @default.
• W3134737776 cites W2752317959 @default.
• W3134737776 cites W2783610221 @default.
• W3134737776 cites W2789370120 @default.
• W3134737776 cites W2790791586 @default.
• W3134737776 cites W2802128468 @default.
• W3134737776 cites W2898958312 @default.
• W3134737776 cites W2951083276 @default.
• W3134737776 cites W2963316476 @default.
• W3134737776 cites W2969593367 @default.
• W3134737776 cites W2973065472 @default.
• W3134737776 cites W2995314688 @default.
• W3134737776 cites W3022482893 @default.
• W3134737776 cites W4252572507 @default.
• W3134737776 doi "https://doi.org/10.1016/j.algal.2021.102246" @default.
• W3134737776 hasPublicationYear "2021" @default.
• W3134737776 type Work @default.
• W3134737776 sameAs 3134737776 @default.
• W3134737776 citedByCount "14" @default.
• W3134737776 countsByYear W31347377762021 @default.
• W3134737776 countsByYear W31347377762022 @default.
• W3134737776 countsByYear W31347377762023 @default.
• W3134737776 crossrefType "journal-article" @default.
• W3134737776 hasAuthorship W3134737776A5015782442 @default.
• W3134737776 hasAuthorship W3134737776A5045504835 @default.
• W3134737776 hasAuthorship W3134737776A5072331448 @default.
• W3134737776 hasAuthorship W3134737776A5083534218 @default.
• W3134737776 hasAuthorship W3134737776A5089507879 @default.
• W3134737776 hasAuthorship W3134737776A5091038325 @default.
• W3134737776 hasConcept C104317684 @default.
• W3134737776 hasConcept C121050878 @default.
• W3134737776 hasConcept C150194340 @default.
• W3134737776 hasConcept C171250308 @default.
• W3134737776 hasConcept C181199279 @default.
• W3134737776 hasConcept C185592680 @default.
• W3134737776 hasConcept C18903297 @default.
• W3134737776 hasConcept C192562407 @default.
• W3134737776 hasConcept C19924922 @default.
• W3134737776 hasConcept C199360897 @default.
• W3134737776 hasConcept C26517878 @default.
• W3134737776 hasConcept C2778411929 @default.
• W3134737776 hasConcept C2780841128 @default.

• next