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- W3134741535 abstract "Phosphorylated metabolites are omnipresent in cells, but their analytical characterization faces several technical hurdles. Here, we detail an improved NMR workflow aimed at assigning the high-resolution subspectrum of the phospho-metabolites in a complex mixture. Combining a pure absorption J-resolved spectrum (Pell, A. J.; J. Magn. Reson. 2007, 189 (2), 293−299) with alternate on- and off-switching of the 31P coupling interaction during the t1 evolution with a pure in-phase (PIP) HSQMBC experiment (Castañar, L.; Angew. Chem., Int. Ed. 2014, 53 (32), 8379−8382) without or with total correlation spectroscopy (TOCSY) transfer during the insensitive nuclei enhancement by polarization transfer (INEPT) gives access to selective identification of the individual subspectra of the phosphorylated metabolites. Returning to the initial J-res spectra, we can extract with optimal resolution the full trace for the individual phospho-metabolites, which can then be transposed on the high-resolution quantitative one dimensional spectrum." @default.
- W3134741535 created "2021-03-15" @default.
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- W3134741535 date "2021-03-12" @default.
- W3134741535 modified "2023-10-16" @default.
- W3134741535 title "Improved NMR Detection of Phospho-Metabolites in a Complex Mixture" @default.
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- W3134741535 doi "https://doi.org/10.1021/acs.analchem.0c04056" @default.
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